| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2017: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
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| Outline of Final Research Achievements |
Development of elastic substrate such as hydrogels to assure the stable maintenance and proliferation of human iPS cells is strongly required for tissue engineering field. In this study, based on our original methodology of microelasticity pattering of hydrogel surface, the most optimal mechanical condition of culture substrate for human iPS cells has been scrutinized. We have found that iPS cells well proliferate on the hydrogel with optimal middle stiffness, and tend to move towards the optimal region to form colonies with maximum expression of undifferentiation makers. These findings should provide an essential principle for designing elastic substrate for high-qualified iPS cell cultures.
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