Innovation and clinical application of lung cancer screening modality using microbead-based technology

• Project/Area Number: 15H03034
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical systems
• Research Institution: Kawasaki Medical School (2017); Okayama University (2015-2016)
• Principal Investigator: NAGASAKA TAKESHI 川崎医科大学, 医学部, 准教授 (30452569)
• Co-Investigator(Kenkyū-buntansha): 豊岡 伸一 岡山大学, 医歯薬学総合研究科, 教授 (30397880) ; 香川 俊輔 岡山大学, 大学病院, 准教授 (00362971) ; 楳田 祐三 岡山大学, 大学病院, 助教 (10573735) ; 母里 淑子 岡山大学, 医歯薬学総合研究科, 助教 (70708081)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2015: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
• Keywords: 医用システム / 検査・診断システム / 腫瘍診断学 / 腫瘍検査学 / エピゲノム / 癌 / バイオテクノロジー

Outline of Final Research Achievements

In Japan, chest X-ray and sputum cytology examination are recommended to lung cancer screening, and in western countries the low dose CT is recommended. However, the accuracy to screen lung cancer is varied widely for the reasons that skills of trained physicians and technicians for chest X-ray, low dose CT and sputum cytology examination are also varied. In this study, we developed a technique to accurately and mechanically detect the tumor-specific alteration, such as aberrant DNA methylation observed on tumor-derived free nucleic acid in sputum and tried to innovate the current lung cancer screening tool based on analyzing methylation status in 76 CpG. We analyzed two independent cohorts by our new developing assay and obtained extremely high precision and reproducibility with AUC of each ROC curve being 0.93 or more.

Research Products

• [Journal Article] Accuracy of four mononucleotide-repeat markers for the identification of DNA mismatch-repair deficiency in solid tumors (2018)
  • Author(s): Takehara Y, Nagasaka T, Nyuya A, Haruma T, Haraga J, Mori Y, Nakamura K, Fujiwara T, Boland CR, Goel A.
  • Journal Title: J Transl Med. Volume: 16 Issue: 1 Pages: 5-5
  • DOI: 10.1186/s12967-017-1376-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparison of outcomes between symptomatic and asymptomatic patients with colorectal cancer: a propensity score-matched analysis of surgical invasiveness, medical costs and oncological outcomes. (2018)
  • Author(s): Inada R, Nagasaka T, Watanabe A, Yagi T, Mori Y, Kondo Y, Kishimoto H, Umeda Y, Fujiwara T.
  • Journal Title: BMJ Open Gastroenterol. Volume: 3;4(1) Issue: 1 Pages: e000146-e000146
  • DOI: 10.1136/bmjgast-2017-000146
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Clinical outcomes of women with ovarian metastases of colorectal cancer treated with oophorectomy with respect to their somatic mutation profiles. (2018)
  • Author(s): Mori Y, Nyuya A, Yasui K, Toshima T, Kawai T, Taniguchi F, Kimura K, Inada R, Nishizaki M, Haraga J, Nakamura K, Umeda Y, Kishimoto H, Fujiwara T, Katata Y, Yamaguchi Y, Nagasaka T.
  • Journal Title: Oncotarget Volume: 27;9(23) Issue: 23 Pages: 16477-16488
  • DOI: 10.18632/oncotarget.24735
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Clinical impact of endometrial cancer stratified by genetic mutational profiles, POLE mutation, and microsatellite instability. (2018)
  • Author(s): Haruma T, Nagasaka T, Nakamura K, Haraga J, Nyuya A, Nishida T, Goel A, Masuyama H, Hiramatsu Y
  • Journal Title: PLoS One Volume: 13 Issue: 4 Pages: e0195655-e0195655
  • DOI: 10.1371/journal.pone.0195655
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Risk Analysis for Invasive Fungal Infection after Living Donor Liver Transplantation: Which Patient Needs Potent Prophylaxis? (2018)
  • Author(s): Utsumi Masashi、Umeda Yuzo、Yagi Takahito、Nagasaka Takeshi、Shinoura Susumu、Yoshida Ryuich、Nobuoka Daisuke、Kuise Takashi、Fuji Tomokazu、Takagi Kosei、Takaki Akinobu、Fujiwara Toshiyoshi
  • Journal Title: Digestive Surgery Volume: 36 Issue: 1 Pages: 59-66
  • DOI: 10.1159/000486548
  • Peer Reviewed
• [Journal Article] Antitumor activity of pan-HER inhibitors in HER2-positive gastric cancer. (2018)
  • Author(s): Yoshioka T, Shien K, Namba K, Torigoe H, Sato H, Tomida S, Yamamoto H, Asano H, Soh J, Tsukuda K, Nagasaka T, Fujiwara T, Toyooka S.
  • Journal Title: Cancer Sci. Volume: 109 Issue: 4 Pages: 1166-1176
  • DOI: 10.1111/cas.13546
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Novel evidence for a PIWI-interacting RNA (piRNA) as an oncogenic mediator of disease progression, and a potential prognostic biomarker in colorectal cancer. (2018)
  • Author(s): Weng W, Liu N, Toiyama Y, Kusunoki M, Nagasaka T, Fujiwara T, Wei Q, Qin H, Lin H, Ma Y, Goel A.
  • Journal Title: Mol Cancer Volume: 30 Pages: 16-16
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Living Donor Liver Transplantation for Acute Liver Failure : Comparing Guidelines on the Prediction of Liver Transplantation. (2017)
  • Author(s): Yoshida K, Umeda Y, Takaki A, Nagasaka T, Yoshida R, Nobuoka D, Kuise T, Takagi K, Yasunaka T, Okada H, Yagi T, Fujiwara T.
  • Journal Title: Acta Med Okayama Volume: 71(5) Pages: 381-390
  • DOI: 10.3892/ol.2017.7553
  • NAID: 120006352357
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Living Donor Liver Transplantation for Acute Liver Failure : Comparing Guidelines on the Prediction of Liver Transplantation (2017)
  • Author(s): Yoshida K, Umeda Y, Takaki A, Nagasaka T, Yoshida R, Nobuoka D, Kuise T, Takagi K, Yasunaka T, Okada H, Yagi T, Fujiwara T.
  • Journal Title: Acta Medica Okayama Volume: 71 Issue: 5 Pages: 381-390
  • DOI: 10.18926/AMO/55435
  • NAID: 120006352357
  • URL: https://ousar.lib.okayama-u.ac.jp/55435
  • Peer Reviewed
• [Journal Article] SNORA21 - An Oncogenic Small Nucleolar RNA, with a Prognostic Biomarker Potential in Human Colorectal Cancer. (2017)
  • Author(s): Yoshida K, Toden S, Weng W, Shigeyasu K, Miyoshi J, Turner J, Nagasaka T, Ma Y, Takayama T, Fujiwara T, Goel A.
  • Journal Title: EBioMedicine Volume: 22 Pages: 68-77
  • DOI: 10.1016/j.ebiom.2017.07.009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Circular RNA ciRS-7 - A promising prognostic biomarker and a potential therapeutic target in colorectal cancer (2017)
  • Author(s): Weng W, Wei Q, Toden S, Yoshida K, Nagasaka T, Fujiwara T, Cai S, Qin H, Ma Y, Goel A
  • Journal Title: Clin Cancer Res. Volume: in press Issue: 14 Pages: 3918-3928
  • DOI: 10.1158/1078-0432.ccr-16-2541
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer. (2017)
  • Author(s): Okugawa Y, Toiyama Y, Toden S, Mitoma H, Nagasaka T, Tanaka K, Inoue Y, Kusunoki M, Boland CR, Goel A.
  • Journal Title: Gut Volume: 66 Issue: 1 Pages: 107-117
  • DOI: 10.1136/gutjnl-2015-309359
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Adenocarcinoma in the jejunum 20 years after surgery for familial adenomatous polyposis (2017)
  • Author(s): Yuusaku Sugihara. Seiji Kawano, Keita Harada, Shiho Takashima, Daisuke Takei, Toshihiro Inokuchi, Masahiro Takahara, Sakiko Hiraoka, Yoshiko Mori, Hiroyuki Kishimoto, Takeshi Nagasaka, Hiroyuki Okada
  • Journal Title: Okayama Igakkai Zasshi (Journal of Okayama Medical Association) Volume: 129 Issue: 2 Pages: 111-114
  • DOI: 10.4044/joma.129.111
  • NAID: 130006039373
  • ISSN: 0030-1558, 1882-4528
  • Peer Reviewed
• [Journal Article] Microsatellite Alterations With Allelic Loss at 9p24.2 Signify Less-Aggressive Colorectal Cancer Metastasis. (2016)
  • Author(s): Koi M, Garcia M, Choi C, Kim HR, Koike J, Hemmi H, Nagasaka T, Okugawa Y, Toiyama Y, Kitajima T, Imaoka H, Kusunoki M, Chen YH, Mukherjee B, Boland CR, Carethers JM.
  • Journal Title: Gastroenterology Volume: 150(4) Issue: 4 Pages: 944-955
  • DOI: 10.1053/j.gastro.2015.12.032
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Protocol of a randomised phase III clinical trial of sequential capecitabine or 5-fluorouracil plus bevacizumab (Cape/5-FU-Bmab) to capecitabine or 5-fluorouracil plus oxaliplatin plus bevacizumab (CapeOX/mFOLFOX6-Bmab) versus combination CapeOX/mFOLFOX6-Bmab in advanced colorectal cancer: the C-cubed (C3) study. (2016)
  • Author(s): Nagasaka T, Mishima H, Sawaki A, Shimokawa M, Inukai M, Shinozaki K, Tanioka H, Nasu J, Nishina T, Hazama S, Okajima M, Yamaguchi Y.
  • Journal Title: BMJ open Volume: 6(6) Issue: 6 Pages: e011454-e011454
  • DOI: 10.1136/bmjopen-2016-011454
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Neutrophil to lymphocyte ratio in the pre-treatment phase of final-line chemotherapy predicts the outcome of patients with recurrent ovarian cancer. (2016)
  • Author(s): Nakamura K, Nagasaka T, Nishida T, Haruma T, Ogawa C, Kusumoto T, Seki N, Hiramatsu Y.
  • Journal Title: Oncology letters Volume: 11(6) Issue: 6 Pages: 3975-3981
  • DOI: 10.3892/ol.2016.4513
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Long-Term Survival after Radiofrequency Ablation of Lung Oligometastases from Five Types of Primary Lesions: A Retrospective Evaluation. (2016)
  • Author(s): Omae K, Hiraki T, Gobara H, Iguchi T, Fujiwara H, Matsui Y, Toyooka S, Nagasaka T, Kanazawa S.
  • Journal Title: Journal of vascular and interventional radiology : JVIR Volume: 27(9) Issue: 9 Pages: 1362-1370
  • DOI: 10.1016/j.jvir.2016.05.017
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Risk Factors for the Development of Desmoid Tumor After Colectomy in Patients with Familial Adenomatous Polyposis: Multicenter Retrospective Cohort Study in Japan. (2016)
  • Author(s): Saito Y, Hinoi T, Ueno H, Kobayashi H, Konishi T, Ishida F, Yamaguchi T, Inoue Y, Kanemitsu Y, Tomita N, Matsubara N, Komori K, Kotake K, Nagasaka T, Hasegawa H, Koyama M, Ohdan H, Watanabe T, Sugihara K, Ishida H.
  • Journal Title: Annals of surgical oncology Volume: 23 Issue: S4 Pages: 559-565
  • DOI: 10.1245/s10434-016-5380-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Single Center Prospective Phase II Trial of CT-guided Radiofrequency Ablation for Pulmonary Metastases from Colorectal Cancer (SCIRO-1401). (2016)
  • Author(s): Sakurai J, Matsui Y, Hiraki T, Iguchi T, Fujiwara H, Gobara H, Mitsuhashi T, Nagasaka T, Kanazawa S.
  • Journal Title: Acta medica Okayama Volume: 70(4) Pages: 317-321
  • NAID: 120005828924
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Anti-high mobility group box 1 monoclonal antibody improves ischemia/reperfusion injury and mode of liver regeneration after partial hepatectomy. (2016)
  • Author(s): Sugihara M, Sadamori H, Nishibori M, Sato Y, Tazawa H, Shinoura S, Umeda Y, Yoshida R, Nobuoka D, Utsumi M, Ohno K, Nagasaka T, Yoshino T, Takahashi HK, Yagi T, Fujiwara T.
  • Journal Title: American Journal of Surgery Volume: 211 Issue: 1 Pages: 179-188
  • DOI: 10.1016/j.amjsurg.2015.06.025
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Prevalence of laparoscopic surgical treatment and its clinical outcomes in patients with familial adenomatous polyposis in Japan (2016)
  • Author(s): Ueno H, Kobayashi H, Konishi T, Ishida F, Yamaguchi T, Hinoi T, Kanemitsu Y, Inoue Y, Tomita N, Matsubara N, Komori K, Ozawa H, Nagasaka T, Hasegawa H, Koyama M, Akagi Y, Yatsuoka T, Kumamoto K, Kurachi K, Tanakaya K, Yoshimatsu K, Watanabe T, Sugihara K, Ishida H.
  • Journal Title: International journal of clinical oncology Volume: 21(4) Issue: 4 Pages: 713-722
  • DOI: 10.1007/s10147-016-0953-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Expansion of epigenetic alterations in EFEMP1 promoter predicts malignant formation in pancreatobiliary intraductal papillary mucinous neoplasms. (2016)
  • Author(s): Yoshida K, Nagasaka T, Umeda Y, Tanaka T, Kimura K, Taniguchi F, Fuji T, Shigeyasu K, Mori Y, Yanai H, Yagi T, Goel A, Fujiwara T.
  • Journal Title: J Cancer Res Clin Oncol. Volume: - Issue: 7 Pages: 1-13
  • DOI: 10.1007/s00432-016-2164-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Feasibility of sequential adjuvant chemotherapy with a 3-month oxaliplatin-based regimen followed by 3 months of capecitabine in patients with stage III and high-risk stage II colorectal cancer: JSWOG-C2 study. (2016)
  • Author(s): Tsuruta A, Yamashita K, Tanioka H, Tsuji A, Inukai M, Yamakawa T, Yamatsuji T, Yoshimitsu M, Toyota K, Yamano T, Nagasaka T, Okajima M.
  • Journal Title: Drug design, development and therapy Volume: 10 Pages: 3827-3835
  • DOI: 10.2147/dddt.s112322
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genetic and epigenetic alterations of netrin-1 receptors in gastric cancer with chromosomal instability. (2015)
  • Author(s): Toda K, Nagasaka T, Umeda Y, Tanaka T, Kawai T, Fuji T, Taniguchi F, Yasui K, Kubota N, Takehara Y, Tazawa H, Kagawa S, Sun DS, Nishida N, Goel A, Fujiwara T.
  • Journal Title: Clin Epigenetics Volume: 7 Issue: 1 Pages: 73-73
  • DOI: 10.1186/s13148-015-0096-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Biological ablation of sentinel lymph node metastasis in submucosally invaded early gastrointestinal cancer. (2015)
  • Author(s): Kikuchi S, Kishimoto H, Tazawa H, Hashimoto Y, Kuroda S, Nishizaki M, Nagasaka T, Shirakawa Y, Kagawa S, Urata Y, Hoffman RM, Fujiwara T.
  • Journal Title: Mol Ther. Volume: 23(3) Issue: 4 Pages: 501-509
  • DOI: 10.1136/gutjnl-2014-306957
  • NAID: 120005537574
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Clinical Significance of MLH1 Methylation and CpG Island Methylator Phenotype as Prognostic Markers in Patients with Gastric Cancer. (2015)
  • Author(s): Shigeyasu K, Nagasaka T, Mori Y, Yokomichi N, Kawai T, Fuji T, Kimura K, Umeda Y, Kagawa S, Goel A, Fujiwara T
  • Journal Title: PLoS One Volume: 10 Issue: 6 Pages: e0130409-e0130409
  • DOI: 10.1371/journal.pone.0130409
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The rare BRAF VK600-601E mutation as a possible indicator of poor prognosis in rectal carcinoma - a report of a case. (2015)
  • Author(s): Mori Y, Nagasaka T, Mishima H, Umeda Y, Inada R, Kishimoto H, Goel A, Fujiwara T.
  • Journal Title: BMC medical genetics Volume: 16 Issue: 1 Pages: 1-1
  • DOI: 10.1186/s12881-015-0144-7
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Primary Tumor Location と Genetic/Epigenetic 変異分布不均衡に対する検討 (2018)
  • Author(s): 永坂 岳司1、堅田 洋佑1、岡脇 誠1、山村 真弘1、母里 淑子2、安井 和也2、戸嶋 俊明2、岸本 浩行2、藤原 俊儀2、山口 佳之1
  • Organizer: 第８８回大腸癌研究会
• [Presentation] 大腸癌1070例の遺伝子変異情報を含めた臨床予後解析結果 (2017)
  • Author(s): 永坂 岳司（岡山大学、川崎医科大学） [共著者] 母里 淑子（岡山大学） 重安 邦俊（岡山大学） 河合 毅（岡山大学） 岸本 浩行（岡山大学） 藤原 俊儀（岡山大学） 山口 佳之（川崎医科大学）
  • Organizer: 第72回日本大腸肛門病学会学術集会
• [Presentation] A Novel Circulating Cell Free DNA-Based Assay Can Predict Tumor Response to Systematic Chemotherapy (2017)
  • Author(s): T. Toshima, T. Nagasaka, Y. Mori, T. Kawai, K. Yasui, T. Fuji, F. Taniguchi, K. Kimura, H. Kishimoto, Y. Umeda, A. Goel, T. Fujiwara
  • Organizer: ESMO 2017 Congress
  • Int'l Joint Research
• [Presentation] Heterogenity of epigenetic and EMT marks observed in hepatocellular carcinoma with keratin 19 proficiency (2017)
  • Author(s): Nagasaka T, Okada T, Yokomichi N, Taniguchi F, Toshima T, Yasui K, Fuji T, Mori Y, Kawai T, Umeda Y, Yagi T, Fujiwara T, Goel A
  • Organizer: ESMO 2017 Congress
  • Int'l Joint Research
• [Presentation] Multicenter phase II study of biweekly XELIRI plus Bevacizumab as a second-line therapy in patients with metastatic colorectal cancer (JSWOG-C3 study) (2017)
  • Author(s): Y. Mori, N. Suzuki, T. Nagasaka, H. Tanioka, Y. Iwamoto, Y. Neki, T. Yamatsuji, M. Kobayashi, M. Nakajima, Y. Ojima, S. Ikeda, K. Kawamoto, K. Shinozaki, A. Tsuji, T. Hinoi, Y. Yamaguchi, K. Yamashita, M. Shimokawa, M. Okajima, S. Hazama
  • Organizer: ESMO 2017 Congress
  • Int'l Joint Research
• [Presentation] Gene expression signatures in BRAF V600E mutant colorectal cancer in relation to WNT signaling cascade (2017)
  • Author(s): asui K, Nagasaka T, Nyuya A, Toshima T, Kawai T, Shigeyasu K, Yano S, Mori Y, Haraga J, Nakamura K, Umeda Y, Goel A, Fujiwara T
  • Organizer: ESMO 2017 Congress
  • Int'l Joint Research
• [Presentation] POLE mutations and MSI were positive predictive factors for progression free survival in endometrial cancer patients at the risk of recurrence (2017)
  • Author(s): A. Nyuya, J. Haraga, Takeshi Nagasaka, K. Nakamura, T. Haruma, T. Nishida, K. Yasui, T. Fujiwara, Ajay Goel, H. Masuyama
  • Organizer: ESMO 2017 Congress
  • Int'l Joint Research
• [Presentation] Significance of MSH2 promoter methylation in endometrial cancer with MSH2 deficiency (2017)
  • Author(s): J. Haraga, Takeshi Nagasaka, K. Nakamura, A. Nyuya, T. Haruma, T. Nishida, K. Yasui, T. Fujiwara, Ajay Goel, H. Masuyama
  • Organizer: ESMO 2017 Congress
  • Int'l Joint Research
• [Presentation] Fine Mapping of a Critical Genetic Element for Liver Metasasis from Primary Colorectal Cancer (CRC) within the HIF3A Locus (2017)
  • Author(s): Carlos Zavala, Minoru Koi, Takahito Kitajima, Kazuya Yasui, Takeshi Nagasaka, Chan Choi, Hyeong-Rok Kim, Yuji Toiyama, John M. Carethers
  • Organizer: DDW2017
  • Int'l Joint Research
• [Presentation] Ovarian metastasectomy in colorectal cancer may improve the clinical outcomes of patients with metastatic colorectal cancer regardless of BRAF or KRAS mutational profiles (2017)
  • Author(s): Yoshiko Mori, Takeshi Nagasaka, Hiroaki Tanioka, Akihiro Nyuya, Takashi Kawai, Toshiaki Toshima, Kazuya Yasui, Kunitoshi Shigeyasu, Hiroyuki Kishimoto, Yuzo Umeda, Toshiyoshi Fujiwara
  • Organizer: ESMO-GI 2017
  • Int'l Joint Research
• [Presentation] The clinical predictor of antitumor efficacy of cetuximab retreatment in patients with metastatic colorectal cancer who had a clinical benefit in response to prior cetuximab based regimen (2017)
  • Author(s): Hiroaki Tanioka, Motoi Asano, Ryousuke Yoshida, Naohisa Waki, Masahiro Ishizaki, Kazuki Yamashita, Yoshiko Mori, Takeshi Nagasaka,
  • Organizer: ESMO-GI 2017
  • Int'l Joint Research
• [Presentation] Novel theranostic strategy against scirrhous gastric cancer; combination of chemotherapy and fluorescence oncolytic adenovirus (2017)
  • Author(s): Wataru Ishikawa, Satoru Kikuchi, Hiroshi Tazawa, Shinji Kuroda, Kazuhiro Noma, Hiroyuki Kishimoto, Takeshi Nagasaka, Masahiko Nishizaki, Shunsuke Kagawa, Toshiyoshi Fujiwara
  • Organizer: AACR2017
  • Int'l Joint Research
• [Presentation] Novel evidence for AZIN1 RNA editing-mediated oncogenic role in colorectal cancer (2017)
  • Author(s): Kunitoshi Shigeyasu, Shusuke Toden, Yoshinaga Okugawa, Jinsei Miyoshi, Takeshi Nagasaka, Toshiyoshi Fujiwara, Leilei Chen, Ajay Goel
  • Organizer: AACR2017
  • Int'l Joint Research
• [Presentation] Gene expression profiles in BRAF V600E mutant colorectal cancer and association with SFRP2 methylation status (2017)
  • Author(s): Kazuya Yasui, Takeshi Nagasaka, Toshiaki Toshima, Takashi Kawai, Kunitoshi Shigeyasu, Yoshiko Mori, Junko Haraga, Keiichiro Nakamura, Yuzo Umeda, Hiroshi Tazawa, Ajay Goel, Toshiyoshi Fujiwara
  • Organizer: AACR2017
  • Int'l Joint Research
• [Presentation] Fluorescence-guided spatiotemporal dynamics of epithelial-mesenchymal transition under inflammatory microenvironment during colorectal cancer progression (2017)
  • Author(s): Takeshi Ieda, Hiroshi Tazawa, Satoru Kikuchi, Shinji Kuroda, Toshiaki Ohara, Kazuhiro Noma, Hiroyuki Kishimoto, Takeshi Nagasaka, Masahiko Nishizaki, Shunsuke Kagawa, Takeshi Imamura, Toshiyoshi Fujiwara
  • Organizer: AACR2017
  • Int'l Joint Research
• [Presentation] Clinicopathological significance of endometrial cancer with MSH2 deficiency (2017)
  • Author(s): Junko Haraga, Takeshi Nagasaka, Keiichirou Nakamura, Tomoko Haruma, Takeshi Nishida, Akihiro Nyuya, Kazuya Yasui, Hisashi Masuyama, Toshiyoshi Fujiwara, Yuji Hiramatsu
  • Organizer: AACR2017
  • Int'l Joint Research
• [Presentation] Hyperthermia at the single-cell level for disseminated cancer disease with immuno-magnetic nanoparticles (2017)
  • Author(s): Tetsuya Kagawa, Hiroyuki Kishimoto, Toshiaki Ohara, Hiroshi Tazawa, Shunsuke Kagawa, Takeshi Nagasaka, Satoshi Nohara, Ichiro Kato, Adarsh Sandhu, Hiromichi Aono, Toshiyoshi Fujiwara
  • Organizer: AACR2017
  • Int'l Joint Research
• [Presentation] 遺伝子変異情報に基づいた大腸癌肝転移の治療戦略―原発巣の左右局在とRAS/RAF 変異の意義― (2017)
  • Author(s): 楳田祐三, 永坂岳司, 母里淑子, 吉田龍一, 信岡大輔, 杭瀬 崇, 藤 智和, 河合 毅, 荒木宏之, 八木孝仁, 藤原俊義
  • Organizer: 第117回日本外科学会定期学術集会
• [Presentation] StageIV大腸癌に対するPrecision Medicineの構築 (2017)
  • Author(s): 河合 毅, 永坂岳司, 母里淑子, 楳田祐三, 稲田 涼, 谷口文崇, 安井和也, 戸嶋俊明, 岸本浩行, 藤原俊義
  • Organizer: 第117回日本外科学会定期学術集会
• [Presentation] utcome of locally advanced rectal cancer with or without preoperative treatments (2017)
  • Author(s): 母里淑子, 永坂岳司, 稲田 涼, 河合 毅, 近藤喜太, 浅野博昭, 佃 和憲, 木村圭佑, 楳田祐三, 岸本浩行, 藤原俊義
  • Organizer: 第117回日本外科学会定期学術集会
• [Presentation] 便中メチル化CpG検出による非侵襲的大腸癌スクリーニング技術の実用化を目指して (2017)
  • Author(s): 永坂岳司, 母里淑子, 入谷光洋, 安井和也, 戸嶋俊明, 河合 毅, 重安邦俊, 谷口文崇, 藤 智和, 楳田祐三, 岸本浩行, 藤原俊義
  • Organizer: 第87回大腸癌研究会
• [Presentation] PVT1 は癌遺伝子MYC を駆動するエンハンサーでありStage II, III 大腸癌の予後予測マーカーになりうる (2017)
  • Author(s): 重安邦俊, 小澤穀士, 松山貴俊, 河合 毅, 矢野修也, 母里淑子, 岸本浩行, 永坂岳司, Goel A, 藤原俊義
  • Organizer: 第72回日本消化器外科学会総会
• [Presentation] 大腸癌多発肝転移におけるRAS/RAF変異の意義～生物学的悪性度から術前化学療法適応を見極める～ (2017)
  • Author(s): 楳田祐三, 八木孝仁, 永坂岳司, 吉田龍一, 信岡大輔, 母里淑子, 杭瀬 崇, 高木弘誠, 河合 毅, 藤原俊義
  • Organizer: 第72回日本消化器外科学会総会
• [Presentation] 局所進行直腸癌に対する術前化学療法の有用性 (2017)
  • Author(s): 母里淑子, 永坂岳司, 河合 毅, 重安邦俊, 矢野修也, 近藤喜太, 楳田祐三, 岸本浩行, 藤原俊義
  • Organizer: 第72回日本消化器外科学会総会
• [Presentation] Lynch Syndrome に対する化学療法 (2017)
  • Author(s): 母里淑子, 永坂岳司, 角田美穂, 山口達郎, 田中屋宏爾, 山下健太郎, 野水整, 檜井孝夫, 石田秀行, 赤木究
  • Organizer: 第15回日本臨床腫瘍学会学術集会
• [Presentation] Clinical prevalence of microsatellite instability colorectal cancer and lynch syndrome cancer (2017)
  • Author(s): 永坂岳司, 母里淑子, 重安邦俊, 原賀順子, 谷口文崇, 藤原俊義
  • Organizer: 第23回家族性腫瘍学会学術集会
• [Presentation] ミスマッチ修復の観点からみた、大腸癌におけるExportin-5 の臨床的意義 (2017)
  • Author(s): 重安邦俊, 奥川喜永, 母里淑子, 安井和也, 入谷光洋, 河合 毅, 矢野修也, 岸本浩行, 永坂岳司, 香川俊輔, Goel A, 藤原俊義
  • Organizer: 第23回家族性腫瘍学会学術集会
• [Presentation] 切除不能進行再発大腸がんの２次治療以降における Ramcirumab + FOLFIRI 療法の治療成績 (2017)
  • Author(s): 永坂岳司, 母里淑子, 重安邦俊, 河合 毅, 矢野修也, 岸本浩行, 楳田祐三, 山口佳之, 藤原俊義
  • Organizer: 第55回日本癌治療学会学術集会
• [Presentation] 遅発性に肝転移・骨盤内リンパ節転移をきたした直腸神経内分泌腫瘍の1例 (2017)
  • Author(s): 重安邦俊, 杭瀬 崇, 母里淑子, 河合 毅, 矢野修也, 戸嶋俊明, 浅野博昭, 近藤喜太, 佃 和憲, 永坂岳司, 八木孝仁, 藤原俊義
  • Organizer: 第72回日本大腸肛門病学会学術集会
• [Presentation] Novel assay for the detection of methylated CpGs from sputum to screen patients with lung cancer (2016)
  • Author(s): Takeshi Nagasaka, Akihiro N, Toshiaki T, Takeshi K, Kazuya Y, Keisuke K, Yoshiko M, Hiroyuki K, Toshiyoshi F
  • Organizer: ESMO Asia 2016
  • Place of Presentation: Singapore
  • Year and Date: 2016-12-16
  • Int'l Joint Research
• [Presentation] 遺伝子変異情報を考慮したStageIV大腸癌に対する予後の検討及び治療戦略の構築 (2016)
  • Author(s): 永坂岳司， 母里淑子， 岸本浩行， 神原健， 河合毅， 中谷紳， 嶋村廣視， 富岡憲明， 森 谷行利， 瀧上隆夫， 佃和憲， 藤原俊義
  • Organizer: 第71回日本大腸肛門病学会学術集会
  • Place of Presentation: 伊勢
  • Year and Date: 2016-11-18
• [Presentation] 当院におけるmFOLFOXIRIの検討 (2016)
  • Author(s): 母里淑子，永坂岳司， 岸本浩行， 河合毅， 佃和憲， 藤原俊義
  • Organizer: 第71回日本大腸肛門病学会学術集会
  • Place of Presentation: 伊勢
  • Year and Date: 2016-11-18
• [Presentation] リンチ症候群における遺伝子変異の特徴とスクリーニング， PD-1 を含めた治療標的 (2016)
  • Author(s): 永坂岳司, 谷口文崇, 母里淑子, 藤原俊義
  • Organizer: 第14回日本臨床腫瘍学会学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2016-07-28
• [Presentation] ランチョンセミナーI 大腸癌の術後補助化学療法 (2016)
  • Author(s): 永坂岳司
  • Organizer: 第25回日本がん転移学会学術集会・総会
  • Place of Presentation: 米子
  • Year and Date: 2016-07-21
  • Invited
• [Presentation] Acquired Resistance to Anti-Epidermal Growth Factor Receptor Therapy in Metastatic Colorectal Cancer (2016)
  • Author(s): 谷口文崇, 永坂岳司, 藤 智和, 母里淑子, 岸本浩行, 河合 毅, 楳田祐三, 藤原俊義
  • Organizer: 第71回日本消化器外科学会総会
  • Place of Presentation: 徳島
  • Year and Date: 2016-07-14
• [Presentation] 4つのモノヌクレオチドリピートマーカーを用いたマイクロサテライト不安定性腫瘍検出方法の検討 (2016)
  • Author(s): 永坂岳司, 竹原裕子, 入谷光洋, 母里淑子, 春間朋子, 原賀順子, 中村圭一郎, 平松祐司, 藤原俊義
  • Organizer: 第22回日本家族性腫瘍学会学術集会
  • Place of Presentation: 松山
  • Year and Date: 2016-06-03
• [Presentation] MGMT Inactivation Arrest Tumor Growth and Serves As a Promising Predictive Biomarker for Treatment Response in Colorectal Cancer (2016)
  • Author(s): Mori Y, Nagasaka T, Tazawa H, Umeda Y, Shigeyasu K, Yoshida K, Goel A, Fujiwara T
  • Organizer: Digestive Disease Week (DDW) 2016
  • Place of Presentation: San Diego
  • Year and Date: 2016-05-21
  • Int'l Joint Research
• [Presentation] A novel circulating cell free DNA-based assay in colorectal cancer patients during treatment with systematic chemotherapy (2016)
  • Author(s): Toshima T, Nagasaka T, Mori Y, Kawai T, Fuji T, Taniguchi F, Kimura K, Yasui K, Kishimoto H, Umeda Y, Tazawa H, Goel A, Fujiwara T
  • Organizer: AACR Annual Meeting 2016
  • Place of Presentation: New Orleans20
  • Year and Date: 2016-04-16
  • Int'l Joint Research
• [Presentation] PD-1 and PD-L1 expression patterns and DNA mismatch repair status for precision management of patients with gastric cancer (2016)
  • Author(s): Kimura K, Nagasaka T, Mori Y, Kawai T, Fuji T, Taniguchi F, Yasui K, Toshima T, Umeda Y, Tazawa H, Goel A, Fujiwara T
  • Organizer: AACR Annual Meeting 2016
  • Place of Presentation: New Orleans
  • Year and Date: 2016-04-16
  • Int'l Joint Research
• [Presentation] Micro RNAs as promising therapeutic targets for anti-metastatic therapy in colorectal cancer (2016)
  • Author(s): Kawai T, Nagasaka T, Mori Y, Fuji T, Taniguchi F, Kimura K, Toshima T, Yasui K, Umeda Y, Tazawa H, Goel A, Fujiwara T
  • Organizer: AACR Annual Meeting 2016
  • Place of Presentation: New Orleans
  • Year and Date: 2016-04-16
  • Int'l Joint Research
• [Presentation] Genome-wide enhancer analysis identifies PVT1 as an oncogenic enhancer of MYC (2016)
  • Author(s): Shigeyasu K, Toden S, Nagasaka T, Fujiwara T, Boland CR, Goel A
  • Organizer: AACR Annual Meeting 2016
  • Place of Presentation: New Orleans
  • Year and Date: 2016-04-16
  • Int'l Joint Research
• [Presentation] A novel live imaging system for inflammation-induced epithelial-mesenchymal transition in colorectal cancers (2016)
  • Author(s): eda T, Tazawa H, Kikuchi S, Kuroda S, Ohara T, Noma K, Kishimoto H, Nagasaka T, Nishizaki M, Kagawa S, Fujiwara T
  • Organizer: AACR Annual Meeting 2016
  • Place of Presentation: New Orleans
  • Year and Date: 2016-04-16
  • Int'l Joint Research
• [Presentation] Comprehensive analysis of intrahepatic cholangiocarcinoma based on viral infections and mutational status in the IDH1/2 and KRAS genes (2016)
  • Author(s): Yasui K, Nagasaka T, Umeda Y, Fuji T, Taniguchi F, Toshima T, Kimura K, Kawai T, Mori Y, Tazawa H, Yagi T, Goel A, Fujiwara T
  • Organizer: AACR Annual Meeting 2016
  • Place of Presentation: New Orleans
  • Year and Date: 2016-04-16
  • Int'l Joint Research
• [Presentation] Clinical significance of SNORA21, an H/ACA box snoRNA, as a metastasis predicting and prognostic biomarker in colorectal cancer (2016)
  • Author(s): Yoshida K, Toden S, Weng W, Nagasaka T, Fujiwara T, Goel A
  • Organizer: AACR Annual Meeting 2016
  • Place of Presentation: New Orleans
  • Year and Date: 2016-04-16
  • Int'l Joint Research
• [Presentation] 切除可能局所進行直腸癌に対する術前化学療法および術前放射線化学療法の検討 (2016)
  • Author(s): 母里淑子, 永坂岳司, 岸本浩行, 河合 毅, 工藤泰崇, 樹下真希, 近藤喜太, 浅野博昭, 佃 和憲, 藤原俊義
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2016-04-14
• [Presentation] 膵癌切除症例における免疫チェックポイント分子の発現解析～膵癌切除例とTCGA dataを対象とした免疫チェックポイント機構の解析～ (2016)
  • Author(s): 藤 智和, 楳田祐三, 永坂岳司, 母里淑子, 谷口文崇, 安井和也, 河合 毅, 八木孝仁, 藤原俊義
  • Organizer: 第116回日本外科学会定期学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2016-04-14
• [Presentation] Clinical Significance of MLH1 Methylation and CpG Island Methylator Phenotype as Prognostic Markers in Patients with Gastric Cancer (2016)
  • Author(s): Mori Y, Shigeyasu K, Nagasaka T, ToyookaS, Fujiwara T
  • Organizer: ICHG2016 第13回国際人類遺伝学会
  • Place of Presentation: 京都
  • Year and Date: 2016-04-03
  • Int'l Joint Research
• [Presentation] Genetic and epigenetic alterations of netrin-1 receptors in gastric cancer (2016)
  • Author(s): Nagasaka T, Mori Y, Shigeyasu K, Toyooka S, Fujiwara T
  • Organizer: ICHG2016 第13回国際人類遺伝学会
  • Place of Presentation: 京都
  • Year and Date: 2016-04-03
  • Int'l Joint Research
• [Presentation] 切除不能進行・再発大腸癌治療におけるBest Practice －抗EGFR抗体薬の使い方－ (2016)
  • Author(s): 永坂岳司
  • Organizer: 第4回岡山大腸腫瘍研究会
  • Place of Presentation: 岡山
  • Invited
• [Presentation] Lynch症候群および家族性大腸腺腫症の系統的遺伝子解析 (2016)
  • Author(s): 谷口文崇, 永坂岳司, 楳田祐三, 母里淑子, 河合 毅, 内海方嗣, 田中屋宏爾, 青木秀樹, 竹内仁司, 藤原俊義
  • Organizer: 第85回大腸癌研究会
  • Place of Presentation: 大阪
• [Presentation] 4つのモノヌクレオチドリピートマーカーのミスマッチ修復タンパク欠損を伴う大腸癌・子宮体癌スクリーニングの精確性の検討 (2016)
  • Author(s): 母里淑子, 永坂岳司, 谷口文崇, 河合 毅, 岸本浩行, 神原 健, 藤原俊義
  • Organizer: 第85回大腸癌研究会
  • Place of Presentation: 大阪
• [Presentation] 喀痰を用いたCpGのメチル化検出による非侵襲的なスクリーニングの開発 (2015)
  • Author(s): 河合 毅, 永坂岳司, 藤 智和, 谷口文崇, 戸嶋俊明, 母里淑子, 豊岡伸一, 藤原俊義
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2015-10-08
• [Presentation] Non invasive detection of methylated CpGs from sputum can predict patiets with lung cancer (2015)
  • Author(s): Kawai T, Nagasaka T, Takehara Y, Umeda Y, Fuji T, Taniguchi F, Kimura K, Toshima T, Yasui K, Mori Y, Kishimoto H, Toyooka S, Goel A, Fujiwara T
  • Organizer: The European Cancer Congress 2015
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2015-09-25
  • Int'l Joint Research