Structural analysis of membrane protein based on multi-labeled sites
| Project/Area Number |
15H03119
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | University of Toyama |
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Principal Investigator |
TOMOHIRO Takenori 富山大学, 大学院医学薬学研究部(薬学), 教授 (70357581)
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| Co-Investigator(Kenkyū-buntansha) |
千葉 順哉 富山大学, 大学院医学薬学研究部(薬学), 助教 (50436789)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIMIZU Takahiro 富山大学, 大学院医学薬学研究部(薬学), 准教授 (40353437)
MATSUNAGA Shigeru 浜松ホトニクス株式会社, 中央研究所, 専任部員 (40391198)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | 化学プローブ / 光アフィニティーラベル / 結合部位解析 / 分子認識 / 標的同定 / バイオテクノロジー / ケミカルバイオロジー / プロテオミクス |
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| Outline of Final Research Achievements |
Multifunctionalization of photocross-linker including fluorogenicity and cleavability has improved efficiency of labeled site analysis and enabled simultaneous analysis of multiple trace amount labels. We have developed a new methodology to monitor the allosteric structural change of protein due to binding of substrates based on label amount change of each labeled amino acid within the ligand-binding domain.
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Report
(4 results)
Research Products
(23 results)
