| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
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| Outline of Final Research Achievements |
We found that acute optogenetic excitation of GABA neurons in the BNST during non-rapid eye movement (NREM) sleep in mice resulted in immediate transition to a wakefulness state without the function of orexins. Prolonged excitation of GABABNST neurons by a chemogenetic method evoked a longer-lasting, sustained wakefulness state, which was abolished by pre-administration of a dual orexin receptor antagonist. We also found that when the orexin→NA→amygdala circuit was artificially activated, fear-related behavior increased in a similar but distinct from the training context. Increased activation of these circuits enhanced the fear response in otherwise neutral situation. Conditions that naturally activate orexin neurons, such as fasting, could result in an orexin-dependent shift to a ‘vigilance’ state and alter the selection of a behavioral response to avoid and handle environmental threats.
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