| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Outline of Final Research Achievements |
By combining a high-repetition-rate nanosecond pulsed laser as a light source, and a high-speed camera as a detector, we constructed a fast structured illumination microscope. A laser pulse was split into two beam lines by using a grating and the structured illumination pattern was obtained on a sample as the interference pattern of two or three beams. The introduction of two Pockels' cells into the beam line(s) enables us to modulate the phase of the illumination pattern electrically within few hundreds microseconds. By the high-speed camera synchronized with the Pockels' cells, we obtained fluorescence images illuminated with different-phase pattern. As the result, we succeeded in obtaining optical sectioned images of a fluorescent stained living cell every 3 ms, and super-resolution images within 5 ms.
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