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Cellular response by the targeted cytoplasmic irradiation in normal human fibroblasts with monochromatic X-ray microbeams

Research Project

Project/Area Number 15H03598
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Quantum beam science
Research InstitutionNational Institutes for Quantum and Radiological Science and Technology

Principal Investigator

Suzuki Masao  国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 放射線障害治療研究部, 上席研究員(定常) (70281673)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords電磁波放射線 / シンクロトロン放射光 / マイクロビーム / バイスタンダー効果 / 放射線適応応答 / 細胞質限定的照射 / ヒト正常細胞 / 細胞増殖死 / 細胞致死効果 / 放射光
Outline of Final Research Achievements

We have been examining the radio-adaptive response induced in the cells pre-irradiated cytoplasm with low-LET electromagnetic radiations. The cytoplasm of all cells captured by the computerized irradiation system was irradiated with 10R first, and then the cell nucleus of all cells captured was irradiated with 10R at the 3hr interval, within which the cells were kept in a CO2 incubator at 37oC. Cell-killing effect was measured with a colony-forming assay as a reproductive dell death. The surviving fraction for the targeted cell nucleus irradiation alone was around 0.80 and no cell-killing effect was induced by the targeted cytoplasm irradiation alone. The surviving fraction in the cells irradiated with the targeted cell nuclei when irradiated the cytoplasm beforehand was increased at 0.96. The obtained results clearly suggest that the radio-adaptive response should occur in the cells pre-irradiated to cytoplasm with the X-ray microbeams.

Academic Significance and Societal Importance of the Research Achievements

マイクロビーム化したX線を細胞核または細胞質に限定的に照射して誘導される生物効果・細胞応答を解明しようとする研究は世界に例のない独創的な研究である。強い強度の放射光X線マイクロビームによる細胞特定部位限定的照射技術の汎用化は、放射線損傷の初期過程から細胞レベルの影響の広範囲に渡る放射線生物影響誘導メカニズムの解明が期待出来るとともに、東日本大震災に伴う福島第一原子力発電所の事故に起因する放射性物質による環境汚染で最も問題となる低線量(率)放射線被ばくの人体影響解明からがんの放射線治療や宇宙放射線の防護など広い応用面にもインパクトを与える研究となることが期待出来る。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (19 results)

All 2019 2018 2017 2016 2015

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Journal Article] Potential lethal damage repair in glioblastoma cells irradiated with ion beams of various types and levels of linear energy transfer.2019

    • Author(s)
      C.M.Tsuey, A.Nisbet, M.Suzuki, N.Matsufuji, T.Murakami, B.Jones, D.A.Bradley
    • Journal Title

      Journal of Radiation Research

      Volume: 60 Issue: 1 Pages: 59-68

    • DOI

      10.1093/jrr/rry081

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhancement of biological effectiveness of carbon-ion beams by applying a longitudinal magnetic field.2019

    • Author(s)
      T.Inaniwa, M.Suzuki, S.Sato, A.Noda, Y.Iwata, N.Kanematsu, T.Shirai, K.Noda
    • Journal Title

      International Journal of Radiation Biology

      Volume: 95 Issue: 6 Pages: 1-6

    • DOI

      10.1080/09553002.2019.1569774

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of carbon ion beam alone or in combination with cisplatin on malignant mesothelioma cells in vitro.2018

    • Author(s)
      1)Sei Sai, Masao Suzuki, Eun Ho Kin, Mitsuhiro Hayashi, Guillaume Vares, Naoyoshi Yamamoto,Tadaaki Miyamoto
    • Journal Title

      Oncotarget

      Volume: 9 Issue: 19 Pages: 14849-14861

    • DOI

      10.18632/oncotarget.23756

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A correlation of long term effects and radiation quality in the progeny of bystander cells after microbeam radiations : The experimenral study of radiotherapy for cancer risk mitigation.2017

    • Author(s)
      Narongchai Autsavapormporn, Teruaki Konishi, Liu Cuihua, Noriko Usami, Tomoo Funayama, Azzam I. Edouard, Masao Suzuki
    • Journal Title

      Journal of Physics

      Volume: 860 Pages: 012026-012026

    • DOI

      10.1088/1742-6596/860/1/012026

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Efficiency of radiation-induced base lesion excision and the order of enzymatic treatment2016

    • Author(s)
      Shiraishi, I., Shikazono, N., Fujii, K. and Yokoya, A.
    • Journal Title

      Int. J. Radiat. Biol.

      Volume: 93 Issue: 3 Pages: 295-302

    • DOI

      10.1080/09553002.2017.1239849

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 細胞質への予めのX線照射がそれに引き続く細胞核への照射効果を低減する(放射線適応応答誘導)。2019

    • Author(s)
      鈴木雅雄、宇佐美徳子、小林克己
    • Organizer
      第32回日本放射光学会年会・放射光科学合同シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] Bystander effects in non-irradiated normal cells via secreted factor(s) to medium from carbon-ion irradiated tumor cells.2018

    • Author(s)
      Masao Suzuki, Yuichiro Yokota, Tomoo Funayama, Michiyo Suzuki, Yasuhiko Kobayashi
    • Organizer
      第77回日本癌学会学術総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細胞核への放射光X線限定的照射による細胞致死効果に対する予めの細胞質へのX線照射による適応応答誘導。2018

    • Author(s)
      鈴木雅雄、宇佐美徳子
    • Organizer
      日本放射線影響学会第61回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 炭素イオンマイクロビーム照射がん細胞と周囲の非照射正常細胞間の液性因子を介したバイスタンダー効果誘導。2018

    • Author(s)
      鈴木雅雄、舟山知夫、横田裕一郎、鈴木芳代、小林泰彦
    • Organizer
      QST高崎研サイエンスフェスタ2018
    • Related Report
      2018 Annual Research Report
  • [Presentation] 放射光マイクロビームを使った生物照射効果研究2018

    • Author(s)
      鈴木雅雄、宇佐美徳子、舟山知夫、横田裕一郎、鈴木芳代、小林泰彦
    • Organizer
      第65回応用物理学会春季学術講演会 放射線分科会シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 放射線の線質に依存したバイスタンダー効果の現状2018

    • Author(s)
      鈴木雅雄
    • Organizer
      中部原子力懇談会平成29年度第3回放射線専門部会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 炭素イオンビームを照射されたヒトがん細胞から非照射正常細胞へのバイスタンダー効果解析2017

    • Author(s)
      鈴木雅雄、舟山知夫、横田裕一郎、鈴木芳代、小林泰彦
    • Organizer
      QST高崎研サイエンスフェスタ2017
    • Related Report
      2017 Annual Research Report
  • [Presentation] Cell-cell communication mediated bystander effects from carbon-ion irradiated tumor to non-irradiated normal cells.2017

    • Author(s)
      Masao Suzuki, Tomoo Funayama, Yuichiro Yokota, Michiyo Suzuki, Yasuhiko Kobayashi
    • Organizer
      日本放射線影響学会第60回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Bystander effects from carbon-ion irradiated tumor to non-irradiated normal cells, simulating spot scanning irradiation.2017

    • Author(s)
      Masao Suzuki, Yuuichiro Yokota, Sei Sai
    • Organizer
      第76回日本癌学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 宇宙放射線の生物影響は細胞質へのエネルギー付与が鍵を握る?2017

    • Author(s)
      鈴木雅雄、宇佐美徳子、舟山知夫、横田裕一郎、鈴木芳代、小林泰彦
    • Organizer
      日本宇宙生物科学会第32回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 遺伝子突然変異における液性因子を介したバイスタンダー効果の核種依存性2016

    • Author(s)
      鈴木雅雄、舟山知夫、横田裕一郎、鈴木芳代、坂下哲哉、小林泰彦
    • Organizer
      日本放射線影響学会第59回大会
    • Place of Presentation
      広島県広島市JMSアステールプラザ
    • Year and Date
      2016-10-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] Secreted factor(s) mediated bystander effects between carbon-ion irradiated tumor and non-irradiated normal cells.2016

    • Author(s)
      鈴木雅雄、崔星
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      神奈川県横浜市パシフィコ横浜
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] Cell-cell communication mediated bystander effects between carbon-ion irradiated tumor and non-irradiated normal cells.2015

    • Author(s)
      Masao Suzuki, Sei Sai
    • Organizer
      The 74th Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      Nagoya Congress Center, Nagoya
    • Year and Date
      2015-10-08
    • Related Report
      2015 Annual Research Report
  • [Presentation] P53-dependent cell-killing effect via bystander effect using carbon-ion microbeams simulating the spot scanning system with pencil beams.2015

    • Author(s)
      Masao Suzuki, Tomoo Funayama, Yuichiro Yokota, Michiyo Suzuki, Hiroko Ikeda, Tetsuya Sakashita,Yasuhiko Kobayashi, Takeshi Murakami
    • Organizer
      15th International Congress of Radiation Research
    • Place of Presentation
      Kyoto International Congress Center, Kyoto
    • Year and Date
      2015-05-25
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2020-03-30  

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