Cellular response by the targeted cytoplasmic irradiation in normal human fibroblasts with monochromatic X-ray microbeams
| Project/Area Number |
15H03598
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Quantum beam science
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| Research Institution | National Institutes for Quantum and Radiological Science and Technology |
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Principal Investigator |
Suzuki Masao 国立研究開発法人量子科学技術研究開発機構, 放射線医学総合研究所 放射線障害治療研究部, 上席研究員(定常) (70281673)
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| Project Period (FY) |
2015-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 電磁波放射線 / シンクロトロン放射光 / マイクロビーム / バイスタンダー効果 / 放射線適応応答 / 細胞質限定的照射 / ヒト正常細胞 / 細胞増殖死 / 細胞致死効果 / 放射光 |
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| Outline of Final Research Achievements |
We have been examining the radio-adaptive response induced in the cells pre-irradiated cytoplasm with low-LET electromagnetic radiations. The cytoplasm of all cells captured by the computerized irradiation system was irradiated with 10R first, and then the cell nucleus of all cells captured was irradiated with 10R at the 3hr interval, within which the cells were kept in a CO2 incubator at 37oC. Cell-killing effect was measured with a colony-forming assay as a reproductive dell death. The surviving fraction for the targeted cell nucleus irradiation alone was around 0.80 and no cell-killing effect was induced by the targeted cytoplasm irradiation alone. The surviving fraction in the cells irradiated with the targeted cell nuclei when irradiated the cytoplasm beforehand was increased at 0.96. The obtained results clearly suggest that the radio-adaptive response should occur in the cells pre-irradiated to cytoplasm with the X-ray microbeams.
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| Academic Significance and Societal Importance of the Research Achievements |
マイクロビーム化したＸ線を細胞核または細胞質に限定的に照射して誘導される生物効果・細胞応答を解明しようとする研究は世界に例のない独創的な研究である。強い強度の放射光Ｘ線マイクロビームによる細胞特定部位限定的照射技術の汎用化は、放射線損傷の初期過程から細胞レベルの影響の広範囲に渡る放射線生物影響誘導メカニズムの解明が期待出来るとともに、東日本大震災に伴う福島第一原子力発電所の事故に起因する放射性物質による環境汚染で最も問題となる低線量（率）放射線被ばくの人体影響解明からがんの放射線治療や宇宙放射線の防護など広い応用面にもインパクトを与える研究となることが期待出来る。
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Report
(5 results)
Research Products
(19 results)
