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Development of 3-pulse super-resolution microscope and its applications for the visualization of the early events of photosynthesis.

Research Project

Project/Area Number 15H03679
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Condensed matter physics I
Research InstitutionOsaka City University

Principal Investigator

Sugisaki Mitsuru  大阪市立大学, 大学院理学研究科, 准教授 (20360042)

Co-Investigator(Kenkyū-buntansha) 近藤 政晴  名古屋工業大学, 工学(系)研究科(研究院), 助教 (20571219)
出羽 毅久  名古屋工業大学, 工学(系)研究科(研究院), 教授 (70335082)
Project Period (FY) 2015-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Keywords光合成 / 顕微鏡法 / 超解像度顕微鏡 / 光物性 / ナノテクノロジー / 量子光学 / 生物物理
Outline of Final Research Achievements

The super-resolution microscopic methodology has been investigated to observe photosynthetic specimens with a spatial resolution beyond the so-called diffraction limit. By means of the laser scanning GSD microscopy, microscopic images of auto-fluorescence from chloroplasts in green algae have been successfully obtained with a spatial resolution of 100nm. On the other hands, unusual increase in fluorescence efficiency of chlorophyll a was observed when the STED microscopy has been employed, while the non-radiative depopulation of the excited state is expected mainly via the stimulated emission process. This anomaly comes from the anti-Stokes fluorescence, probably due to an efficient involvement of low-frequency vibrational modes. Further, 3-pulse four-wave mixing signals pumped with chirped pulses have been examined, where selective excitations of phonon modes and the control of the coupling strength between a photosynthetic pigment and solvent have been attained.

Academic Significance and Societal Importance of the Research Achievements

本研究では,試料の構造に加え,その機能を,高い空間分解能で同時観測する手法の獲得を目指した.これまで広く一般的に行われてきた方法とは異なり,天然の状態のままの緑藻を測定試料とし,試料を染色せずに,超解像度顕微鏡画像の取得に成功したことに意義がある.また,空間分解能に制限がないとされてきた超解像度顕微鏡法において,これまでの常識を覆す真逆の結果が得られ,その機構を解明した点も重要である.

Report

(6 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (25 results)

All 2020 2019 2018 2017 2016 2015 Other

All Int'l Joint Research (6 results) Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results) Presentation (14 results) (of which Int'l Joint Research: 2 results)

  • [Int'l Joint Research] University of Toronto(カナダ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Toronto(カナダ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Toronto(カナダ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Toronto(Canada)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Toronto(Canada)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] University of Glasgow(英国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Transient grating spectroscopy of β-carotene pumped with spectrally chirped pulses2019

    • Author(s)
      Shintaro Ooi
    • Journal Title

      Journal of Physics: Conference Series

      Volume: 印刷中

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anti-Stokes fluorescence from chlorophyll a2019

    • Author(s)
      Hidetoshi Emura
    • Journal Title

      Journal of Physics: Conference Series

      Volume: 印刷中

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] クロロフィルaにおけるアンチストークス蛍光2017

    • Author(s)
      江村 秀俊,杉﨑 満
    • Journal Title

      第28回光物性研究会論文集

      Volume: 28 Pages: 179-182

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Photoprotection Mechanism of Light-Harvesting Antenna Complex from Purple Bacteria2016

    • Author(s)
      D. Kosumi, T. Horibe, M. Sugisaki, R.J. Cogdell, H. Hashimoto
    • Journal Title

      J. Phys. Chem. B

      Volume: 120 Issue: 5 Pages: 951-956

    • DOI

      10.1021/acs.jpcb.6b00121

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Ultrafast coherent spectroscopic investigation on photosynthetic pigment chlorophyll a utilizing 20 fs pulses2015

    • Author(s)
      D. Kosumi, T. Nishiguchi, M. Sugisaki, H. Hashimoto
    • Journal Title

      J. Photochem. Photobiol. A

      Volume: 313 Pages: 72-78

    • DOI

      10.1016/j.jphotochem.2015.06.025

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Presentation] 緑藻の超解像度顕微蛍光観測2020

    • Author(s)
      橋本健人
    • Organizer
      日本物理学会 第75回年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 波形成型した励起光を用いたβ-カロテンの四光波混合信号II2020

    • Author(s)
      三苫重仁
    • Organizer
      日本物理学会 第75回年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] クロロフィルaの顕微蛍光観測 II2019

    • Author(s)
      橋本健人
    • Organizer
      日本物理学会 2019年秋季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 緑藻の超解像度顕微蛍光観測2019

    • Author(s)
      橋本健人
    • Organizer
      第30回 光物性研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] チャープパルスを用いたβ-カロテンの四光波混合信号2019

    • Author(s)
      三苫重仁
    • Organizer
      第30回 光物性研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 波形成型した励起光を用いたβ-カロテンの四光波混合信号2019

    • Author(s)
      三苫重仁
    • Organizer
      日本物理学会 第74回年次大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] クロロフィルaの顕微蛍光観測2019

    • Author(s)
      杉﨑満
    • Organizer
      日本物理学会 第74回年次大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] フタロシアニンおよびクロロフィルの顕微蛍光観測2018

    • Author(s)
      杉﨑満
    • Organizer
      日本物理学会2018年秋季大会(物性)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Transient grating spectroscopy of beta-carotene pumped with spectrally chirped pulses2018

    • Author(s)
      Sigehito Mitoma
    • Organizer
      The 12th International Conference on Excitonic and Photonic Processes in Condensed Matter and Nano Materials
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Anti-Stokes fluorescence from chlorophyll a2018

    • Author(s)
      Hidetoshi Emura
    • Organizer
      The 12th International Conference on Excitonic and Photonic Processes in Condensed Matter and Nano Materials
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] スペクトル分解したβ-カロテンの四光波混合信号 II2017

    • Author(s)
      杉﨑 満
    • Organizer
      日本物理学会72回年次大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] クロロフィルaにおけるアンチストークス蛍光2017

    • Author(s)
      江村 秀俊,杉﨑 満
    • Organizer
      日本物理学会2017年秋季大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] クロロフィルaにおけるアンチストークス蛍光2017

    • Author(s)
      江村 秀俊,杉﨑 満
    • Organizer
      第28回光物性研究会
    • Related Report
      2016 Annual Research Report
  • [Presentation] スペクトル分解したβ-カロテンの四光波混合信号2016

    • Author(s)
      杉﨑 満
    • Organizer
      日本物理学会2016年秋季大会
    • Related Report
      2016 Annual Research Report

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Published: 2015-04-16   Modified: 2022-11-04  

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