| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Outline of Final Research Achievements |
The super-resolution microscopic methodology has been investigated to observe photosynthetic specimens with a spatial resolution beyond the so-called diffraction limit. By means of the laser scanning GSD microscopy, microscopic images of auto-fluorescence from chloroplasts in green algae have been successfully obtained with a spatial resolution of 100nm. On the other hands, unusual increase in fluorescence efficiency of chlorophyll a was observed when the STED microscopy has been employed, while the non-radiative depopulation of the excited state is expected mainly via the stimulated emission process. This anomaly comes from the anti-Stokes fluorescence, probably due to an efficient involvement of low-frequency vibrational modes. Further, 3-pulse four-wave mixing signals pumped with chirped pulses have been examined, where selective excitations of phonon modes and the control of the coupling strength between a photosynthetic pigment and solvent have been attained.
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