Development of a Bio-Raman Microscope to Enable Long Term Monitoring of Single Cells
| Project/Area Number |
15H03821
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
西澤 精一 東北大学, 理学(系)研究科(研究院), 教授 (40281969)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | ラマン顕微鏡 / 細胞 / スペクトル解析 / 分析科学 / 生細胞 / 分化ダイナミクス |
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| Outline of Final Research Achievements |
Recently it became possible to measure Raman spectra of a single live cell using a standard Raman microscope. We developed a bio-Raman microscope in order to monitor the dynamics of cellular differentiation, proliferation, and apoptosis. To do so, living cells were incubated on the microscope stage. Using the bio-Raman microscope, we defined cellular states in a non-destructive and non-labeling manner. The obtained information of cellular conditions is very important to control cellular destinies aiming tissue engineering. Advanced mathematical methods, developed in this project, were found effective to spectral analysis in a general manner.
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Report
(4 results)
Research Products
(8 results)
