Development of an analytical system for quantitative and qualitative variation of miRNA
| Project/Area Number |
15H03830
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Analytical chemistry
|
|---|
| Research Institution | Tokyo University of Pharmacy and Life Science |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
谷 英典 国立研究開発法人産業技術総合研究所, エネルギー・環境領域, 主任研究員 (10635329)
手嶋 紀雄 愛知工業大学, 工学部, 教授 (30292501)
青木 元秀 東京薬科大学, 生命科学部, 助教 (30418917)
リム リーワ 岐阜大学, 工学部, 准教授 (80377689)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2015: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
|
|---|
| Keywords | クロマトグラフィー / モノリスカラム / 機能性RNA / マイクロリアクター / 質量分析 |
|---|
| Outline of Final Research Achievements |
In order to quantitatively analyze non-coding RNAs including micro RNAs, mass spectrometry-based separation system was constructed. We have refined our monolithic column technology and produced a variety of capillary-based analytical tools for chromatographic separation, analyte extraction, and catalytic reaction. We have also developed interface devices for optimal coupling of separation techniques with mass spectrometry. We have integrated such analytical tools, devices and instruments to construct highly-efficient analysis system for the evaluation of quantitative and qualitative variation of non-coding RNAs.
|
Report
(4 results)
Research Products
(57 results)
-
-
-
-
-
-
[Journal Article] Identification of RNA biomarkers for chemical safety screening in mouse embryonic stem cells using RNA deep sequencing analysis2017
Author(s)
Hidenori Tani, Jun-ichi Takeshita, Hiroshi Aoki, Kaoru Nakamura, Ryosuke Abe, Akinobu Toyoda, Yasunori Endo, Sadaaki Miyamoto, Masashi Gamo, Hiroaki Sato, Masaki Torimura
-
Journal Title
PLOS ONE
Volume: 12
Issue: 7
Pages: e0182032-e0182032
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Effect of methyl p-hydroxybenzoate on the culture of mammalian cell2017
Author(s)
Hidenori Tani, Jun-ichi Takeshita, Hiroshi Aoki, Kaoru Nakamura, Ryosuke Abe, Akinobu Toyoda, Yasunori Endo, Sadaaki Miyamoto, Masashi Gamo, Hiroaki Sato, Masaki Torimura
-
Journal Title
Drug Discoveries & Therapeutics
Volume: 11
Issue: 5
Pages: 276-280
DOI
NAID
ISSN
1881-7831, 1881-784X
Related Report
Peer Reviewed / Open Access
-
-
-
[Journal Article] Electrochemical Determination of Synephrine by Hydrophilic Interaction Liquid Chromatography Using a Zwitterionic Monolith Column2016
Author(s)
Sakai, Y., Kotani, A., Umemura, T., Mori, Y., Kusu, F., Yamamoto, K., Hakamata, H.
-
Journal Title
Electroanalysis
Volume: 28
Issue: 9
Pages: 1947-1951
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Analysis of RNA decay factor mediated RNA stability contributions on RNA abundance2015
Author(s)
Sho Maekawa, Naoto Imamachi, Takuma Irie, Hidenori Tani, Kyoko Matsumoto, Rena Mizutani, Katsutoshi Imamura, Miho Kakeda, Tetsushi Yada, Sumio Sugano, Yutaka Suzuki and Nobuyoshi Akimitsu.
-
Journal Title
BMC Genomics
Volume: 16
Issue: 1
Pages: 154-154
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Development of a Separation Column-integrated Nebulizer for Speciation Analysis by ICP-MS2017
Author(s)
Miyashita, S., Takae, S., Fujii, S., Takatsu, A., Umemura, T., Inagaki, K.
Organizer
Asia Pacific Winter Conference 2017
Related Report
Int'l Joint Research
-
-
-
-
-
-
[Presentation] Preparation and Characterization of Organic Polymer-Based Adsorbent with Phenylboronic Acid Functionality for Capturing Biological cis-Diol-Containing Compounds2016
Author(s)
Aoki, M., Matsumoto, N., Bessho, K., Kumata, H., Uchida, T., Umemura, T.
Organizer
the 13th Asian Conference on Analytical Sciences (ASIANALYSIS XIII)
Place of Presentation
The Empress International Convention Center, Chiang Mai
Year and Date
2016-12-08
Related Report
Int'l Joint Research
-
-
-
-
-
-
[Presentation] Dual-Channel Type Concentric Grid Nebulizer for Inductively Coupled Plasma Optical Emission Spectrometry2016
Author(s)
Matsushita, R., Fujii, S., Miyashita, S., Ido, K., Umemura, T., Inagaki, K.
Organizer
RSC Tokyo International Conference 2016
Place of Presentation
Makuhari Messe
Year and Date
2016-09-08
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
