Development of DNA nanostructure based molecular system DNA nanostructure system for cell function control
| Project/Area Number |
15H03837
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
Endo Masayuki 京都大学, 理学研究科, 特定准教授 (70335389)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Keywords | DNAナノテクノロジー / DNAオリガミ / 1分子観察 / 細胞機能制御 / 原子間力顕微鏡 / 脂質膜 / デリバリーシステム / DNAナノ構造体 / 薬物輸送 / 人工受容体 / リポソーム / ゲノム編集 |
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| Outline of Final Research Achievements |
In this research, we developed novel molecular technology mainly aimed at application to cells using DNA nanotechnology. To control the function of the cells, we construct a series of molecular systems by functionalizing nanostructures and nanospaces by precisely designed DNA. We created controllable dynamic nanostructures having opening and closing mechanism in response to light and specific molecules, and investigated the functions using high speed AFM. We created assembly system of DNA nanostructures on lipid membrane. A DNA nanostructure having a needle-like structure which can penetrate the lipid membrane was constructed, and target molecules were incorporated into the liposome. In addition, a novel delivery system to cells was constructed using controllable DNA nanostructures. Through these studies, we will develop novel molecular technologies for control of cell functions using controllable DNA structures and assemblies.
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Report
(4 results)
Research Products
(88 results)
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[Journal Article] Holliday junction resolvases mediate chloroplast nucleoid segregation2017
Author(s)
Kobayashi Y, Misumi O, Odahara M, Ishibashi K, Hirono M, Hidaka K, Endo M, Sugiyama H, Iwasaki H, Kuroiwa T, Shikanai T, Nishimura Y.
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Journal Title
Science
Volume: 356
Issue: 6338
Pages: 631-634
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate.2017
Author(s)
Tomonori Shibata, Yoshihiko Fujita, Hirohisa Ohno, Yuki Suzuki, Karin Hayashi, Kaoru R. Komatsu, Shunsuke Kawasaki, Kumi Hidaka, Shin Yonehara, Hiroshi Sugiyama, Masayuki Endo and Hirohide Saito
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Journal Title
Nature Communications
Volume: 8
Issue: 1
Pages: 540-540
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Self-assembling DNA hydrogel-based delivery of immunoinhibitory nucleic acids to immune cells.2016
Author(s)
Nishida Y, Ohtsuki S, Araie Y, Umeki Y, Endo M, Emura T, Hidaka K, Sugiyama H, Takahashi Y, Takakura Y, Nishikawa M.
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Journal Title
Nanomedicine: Nanotechnology, Biology and Medicine
Volume: 12
Issue: 1
Pages: 123-130
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Optimal Arrangement of Four Short DNA Strands for Delivery of Immunostimulatory Nucleic Acids to Immune Cells2015
Author(s)
Shozo Ohtsuki, Noriyuki Matsuzaki, Kohta Mohri, Masayuki Endo, Tomoko Emura, Kumi Hidaka, Hiroshi Sugiyama, Yuki Takahashi, Kenichi Ishiyama, Norimitsu Kadowaki, Yoshinobu Takakura, Makiya Nishikawa.
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Journal Title
Nucleic Acid Therapeutics
Volume: 25
Issue: 5
Pages: 245-253
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] DNA鎖の拘束によるCas9切断への影響2017
Author(s)
Michael H. Reaz, 日高 久美, Shana J. Sturla, 杉山 弘, 遠藤 政幸
Organizer
日本化学会第97春季年会(2017)
Place of Presentation
慶応大学日吉キャンパス
Year and Date
2017-03-18
Related Report
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[Book] 分子マシンの科学2017
Author(s)
日本化学会
Total Pages
200
Publisher
化学同人
ISBN
9784759813869
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