Creation of micro-RNA-encapsulated artificial viral capsids bearing cell-penetrating ability
| Project/Area Number |
15H03838
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Tottori University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
岩崎 崇 鳥取大学, 農学部, 准教授 (30585584)
三浦 典正 鳥取大学, 医学部, 准教授 (30325005)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Keywords | 人工ウイルスキャプシド / 細胞透過性ペプチド / 自己集合 / 核酸医薬 / 細胞内導入 / ペプチド / 核酸内包 / 細胞透過性 / 生体材料 / マイクロRNA / 細胞膜透過性 |
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| Outline of Final Research Achievements |
We have developed peptide nanocapsules (artificial viral capsids) self-assembled from beta-annulus peptide from tomato bushy stunt virus. In this research project, we developed nucleic acids-encapsulated artificial viral capsids bearing cell-penetrating peptides. We succeeded in synthesizing beta-annulus peptide bearing cell-penetrating His16 peptide at C-terminal, and encapsulating ssDNA into the artificial viral capsids modified with His16. The His16-modified artificial viral capsids were internalized well into HT1080 cells, whereas unmodified capsids hardly internalized. In addition, we have developed encapsulation of ssDNA via disulfide bonds into artificial viral capsids.
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Report
(4 results)
Research Products
(87 results)
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[Presentation] Self-assembled synthetic viral capsids2015
Author(s)
Kazunori Matsuura
Organizer
MSA International Symposium on Organic Chemistry (ISOC) 2015
Place of Presentation
Putra World Trade Centre, Kuala Lumpur (Malaysia)
Year and Date
2015-09-22
Related Report
Int'l Joint Research / Invited
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