| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Outline of Final Research Achievements |
In suspension culture of iPS cells, the shear stress generated during the mixing is expected to promote differentiation of induced pluripotent stem (iPS) cells. This stress on the cell can be controlled by the impeller shape and rotational rate of the cell suspension. However, it is difficult to measure the effect of these operative parameters on the shear stress by experiments. Therefore, we have developed a numerical model to obtain the average and the maximum shear stress in two kinds of stirred tanks and an orbital shaking cylindrical container. Our results showed that the shear stress depended strongly on the type of mixing and lesser extent on the shape of the impeller. The average shear stress is larger in the shaking mode than that in the stirring mode. In contrast, the maximum shear stress is much smaller for the shaking than for stirring, suggesting stirring and shaking should be selectively used depending on the application.
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