Development of innovative immunoassay through the application of quench-release principle to natural antibodies
| Project/Area Number |
15H04191
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | Tokyo Institute of Technology |
|---|
Principal Investigator |
UEDA Hiroshi 東京工業大学, 科学技術創成研究院, 教授 (60232758)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
大室 有紀 (松山有紀) 東京工業大学, 科学技術創成研究院, 助教 (30571088)
董 金華 東京工業大学, 科学技術創成研究院, 助教 (80527838)
鄭 煕陳 東京工業大学, 資源化学研究所, 助教 (70737981)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KOBAYASHI Norihiro 神戸薬科大学, 薬学部, 教授 (90205477)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2015: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
|
|---|
| Keywords | バイオセンサー / バイオテクノロジー / 抗体 / 抗原 / 蛍光 / クエンチ / ケミカルバイオロジー / 部位特異的修飾 / 結合タンパク質 / 蛋白質 / 免疫学 / プロテオーム / マイクロアレイ / タンパク質 |
|---|
| Outline of Final Research Achievements |
Previous immunoassays have drawbacks of many steps and long time consideration. Recently we found a construction principle of recombinant antibody-based fluorescent biosensor Quenchbody (Q-body), and succeeded in detecting many targets from small molecules to large proteins by just mixing with sample. This time, by the close inspection of working principle, we developed several methodologies to turn not only recombinant but also natural antibodies to Q-bodies.
|
Report
(4 results)
Research Products
(85 results)
