Functional dissection of motor circuits by connectomics and optogenetics
| Project/Area Number |
15H04255
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NOSE Akinao 東京大学, 大学院新領域創成科学研究科, 教授 (30260037)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | コネクトミクス / 光遺伝学 / ショウジョウバエ / 運動回路 / カルシウムイメージング / オプトジェネティクス |
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| Outline of Final Research Achievements |
Drosophila larval peristalsis is generated by sequential contractions of body-wall muscles from the posterior to anterior segments. In this study, we used connectomics and optogenetics to identify excitatory premotor interneurons in this system, termed CLIs. These neurons were segmentally arrayed and activated sequentially from the posterior to anterior segments during peristalsis. The CLIs express ChAT, a marker for cholinergic neurons and were active just prior to motoneuronal firing in each segment. Moreover, local activation of CLI1s induced contraction of muscles in the corresponding body segments. Our results suggest that the CLIs directly activate motoneurons sequentially along the segments during larval locomotion.
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Report
(4 results)
Research Products
(25 results)
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[Presentation] Divergent connectivity of homologous command-like neurons mediates segment-specific touch responses in Drosophila2017
Author(s)
Suguru Takagi, Benjamin T. Cocanougher, Sawako Niki, Dohjin Miyamoto, Hiroshi Kohsaka, Hokto Kazama, Richard D. Fetter, James W. Truman, Marta Zlatic, Albert Cardona, Akinao Nose.
Organizer
CSHLMeeting "Neurobiology of Drosophila"
Related Report
Int'l Joint Research
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[Presentation] Divergent connectivity of command-like neurons mediates segment-specific touch responses in Drosophila larvae2017
Author(s)
高木 優, Benjamin Cocanougher, 二木 佐和子, 宮本 道人, 高坂 洋史, 風間 北斗, Richard Fetter, James Truman, Marta Zlatic, Albert Cardona, 能瀬 聡直
Organizer
第17回 東京大学生命科学シンポジウム
Related Report
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[Presentation] Bidirectional feedback circuits regulate propagation of motor activity in opposite directions.2017
Author(s)
Kohsaka, H., Zwart, M.F., Fushiki, A., Fetter, R. D., Truman, J. W., Cardona, A. and Nose, A.
Organizer
第40回日本神経科学大会
Related Report
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[Presentation] A segment-specific sensorimotor pathway mediates touch-triggered backward escape response in Drosophila larvae2017
Author(s)
Suguru Takagi, Benjamin T. Cocanougher, Sawako Niki, Dohjin Miyamoto, Hiroshi Kohsaka, Hokto Kazama, Richard D. Fetter, James W. Truman, Marta Zlatic, Albert Cardona, Akinao Nose
Organizer
第40回日本神経科学大会
Related Report
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[Presentation] Circuit mechanisms that regulate motor pattern in larval Drosophila.2016
Author(s)
Nose, A., Kohsaka, H., Yoon, Y., Takagi, S., Hiramoto, A., Ohura, S. and Niki, S.
Organizer
Behavioral Neurogenetics of Drosophila Larva
Place of Presentation
Janelia Farm Research Campus(USA, Ashburn)
Year and Date
2016-10-25
Related Report
Int'l Joint Research / Invited
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