| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
We have studied expression mechanism of long-term depression (LTD) using a novel experimental method we developed. LTD is a type of synaptic plasticity, which is a cellular basis of learning and memory. The LTD expression is accompanied by the decrease in number of postsynaptic AMPA-type glutamate receptor, which was suggested to be caused by enhancement of endocytosis. We labelled AMPA receptor (AMPAR) with fluorescent protein SEP, and performed live-cell imaging of AMPAR-SEP in cultured hippocampal neurons. The new method enabled us to visualize individual endo- and exocytosis of AMPAR with a high signal to noise ratio and high spatiotemporal resolution. The results indicate that hippocampal LTD is primarily caused not by the enhancement of AMPAR endocytosis but by the suppression of AMPAR exocytosis.
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