Cytoskeleton mechanisms of activity-dependent axon branching

• Project/Area Number: 15H04260
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Osaka University
• Principal Investigator: YAMAMOTO Nobuhiko 大阪大学, 生命機能研究科, 教授 (00191429)
• Co-Investigator(Kenkyū-buntansha): 菅生 紀之 大阪大学, 生命機能研究科, 助教 (20372625)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2017: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 大脳皮質 / 軸索分岐 / RhoGEF / RhoA / 神経活動依存性 / 細胞骨格 / 水平軸索 / 大脳 / 神経活動

Outline of Final Research Achievements

Axon branching is essential for neuronal circuit formation. In the present study, we investigated the role of Rho guanine nucleotide exchange factor (RhoGEF) in axon branch formation in developing cortical neurons. The results demonstrated that multiple RhoGEFs are expressed in the mammalian cortex and are differentially involved in axon branching of cortical neurons via the RhoA pathway.

Research Products

• [Journal Article] Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures (2018)
  • Author(s): Matsumoto N, Yamamoto N
  • Journal Title: J Vis Exp Volume: 133 Issue: 133
  • DOI: 10.3791/56553
  • Peer Reviewed
• [Journal Article] Nucleocytoplasmic Shuttling of Histone Deacetylase 9 Controls Activity-Dependent Thalamocortical Axon Branching (2017)
  • Author(s): Ricardo Alchini, Haruka Sato, Naoyuki Matsumoto, Tomomi Shimogori, Noriyuki Sugo, Nobuhiko Yamamoto
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 6024-6024
  • DOI: 10.1038/s41598-017-06243-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Immunocytochemistry and fluorescence imaging efficiently identify individual neurons with CRISPR/Cas9-mediated gene disruption in primary cortical cultures (2017)
  • Author(s): Hiroto Tsunematsu, Akiko Uyeda, Nobuhiko Yamamoto, Noriyuki Sugo
  • Journal Title: BMC Neuroscience Volume: 18 Issue: 1 Pages: 55-55
  • DOI: 10.1186/s12868-017-0377-9
  • Peer Reviewed / Open Access
• [Journal Article] Genome Stability by DNA polymerase β in Neural Progenitors Contributes Neuronal Differentiation in Cortical Development (2017)
  • Author(s): Kohei Onishi, Akiko Uyeda, Mistuhiro Shida, Teruyoshi Hirayama, Takeshi Yagi, Nobuhiko Yamamoto, Noriyuki Sugo
  • Journal Title: Journal of Neuroscience Volume: 37 Issue: 35 Pages: 8444-8485
  • DOI: 10.1523/jneurosci.0665-17.2017
  • Peer Reviewed
• [Journal Article] Activity-dependent dynamics of the transcription factor CREB in cortical neurons revealed by single-molecule imaging (2017)
  • Author(s): Kitagawa H, Sugo N, Morimatsu M, Arai Y, Yanagida T, Yamamoto N
  • Journal Title: J Neurosci Volume: 37 Issue: 1 Pages: 1-10
  • DOI: 10.1523/jneurosci.0943-16.2017
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Visualization of HDAC9 spatiotemporal subcellular localization in primary neuron cultures (2016)
  • Author(s): Sugo N, Yamamoto N
  • Journal Title: Methods Mol Biol Volume: 1436 Pages: 119-127
  • DOI: 10.1007/978-1-4939-3667-0_9
  • ISBN: 9781493936656, 9781493936670
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Synapse-dependent and independent mechanisms of thalamocortical axon branching are regulated by neuronal activity (2016)
  • Author(s): Matsumoto, N., Hoshiko, M., Sugo, N., Fukazawa, Y. & Yamamoto, N.
  • Journal Title: Developmental Neurobiology Volume: 76 Issue: 3 Pages: 323-336
  • DOI: 10.1002/dneu.22317
  • Peer Reviewed
• [Journal Article] Single-molecule imaging reveals dynamics of CREB transcription factor bound to its target sequence (2015)
  • Author(s): Sugo, N., Morimatsu, M., Arai, Y., Kousoku, Y., Ohkuni, A., Nomura, T., Yanagida, T. & Yamamoto, N.
  • Journal Title: Scientific Report Volume: 5 Issue: 1 Pages: 10662-10662
  • DOI: 10.1038/srep10662
  • Peer Reviewed / Open Access
• [Presentation] Activity-Dependent Mechanisms for Thalamocortical Circuit Formation (2017)
  • Author(s): Yamamoto N
  • Organizer: The 48th NIPS International Symposium
  • Int'l Joint Research / Invited
• [Presentation] 神経活動依存的な視床皮質軸索の分岐制御機構 (2017)
  • Author(s): 山本亘彦
  • Organizer: 第39回神経組織培養研究会
  • Invited
• [Presentation] The role of neuronal activity and co-factors in CREB dynamics in cortical neurons: A single-molecule imaging study (2017)
  • Author(s): Kitagawa H, Sugo N, Yamamoto N
  • Organizer: Cortical Development Conference
  • Int'l Joint Research
• [Presentation] A negative regulator for activity-dependent thalamocortical axon branching (2017)
  • Author(s): Hayano Y, Araki T, Sasaki K, Miyasaka Y, Nakasone T, Hata Y, Yamamoto, N
  • Organizer: Cortical Development Conference
  • Int'l Joint Research
• [Presentation] Real-time luciferase assay reveals the effects of patterned neuronal activity on BDNF expression in the developing cortex (2017)
  • Author(s): Miyasaka Y, Miyagawa H, Yamamoto N
  • Organizer: The 40th Annual Meeting of the Japan Neuroscience Society
• [Presentation] Temporal sequence of contralateral corticofugal projections and gene expression in the midbrain after hemispherectomy (2017)
  • Author(s): Chang L, Yamamoto N
  • Organizer: The 40th Annual Meeting of the Japan Neuroscience Society
• [Presentation] The role of DNA polymerase β in postnatal cortical neuron development (2017)
  • Author(s): Uyeda A, Sugo N, Onishi K, Lyu J, Hirayama T, Yagi T, Yamamoto N
  • Organizer: The 40th Annual Meeting of the Japan Neuroscience Society
• [Presentation] A study of the role of DNA polymerase β in human brain development using induced pluripotent stem cells (2017)
  • Author(s): Tsunematsu H, Sugo N, Kurosawa A, Adachi N, Yamamoto N
  • Organizer: The 40th Annual Meeting of the Japan Neuroscience Society
• [Presentation] KIT LIGAND ACTS AS A NEGATIVE REGULATOR FOR ACTIVITY-DEPENDENT THALAMOCORTICAL AXON BRANCHING (2016)
  • Author(s): Yasufumi Hayano, Takeshi Araki, Kensuke Sasaki, Yumi Miyasaka, Takuma Nakasone, Yoshio Hata, Nobuhiko Yamamoto
  • Organizer: Axon Guidance, synapse formation & regeneration
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 2016-09-20
  • Int'l Joint Research
• [Presentation] ARHGEF18 REGULATES AXON BRANCHING OF CORTICAL UPPER LAYER NEURONS (2016)
  • Author(s): Kensuke Sasaki, Kei Arimoto, Kento Kankawa, Chikayo Terada, Nobuhiko Yamamoto
  • Organizer: Axon Guidance, synapse formation & regeneration
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 2016-09-20
  • Int'l Joint Research
• [Presentation] Positive and negative regulation of activity-dependent thalamocortical axon branching (2016)
  • Author(s): Nobuhiko Yamamoto
  • Organizer: The 39th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
  • Int'l Joint Research / Invited
• [Presentation] DNA polymerase β function in neural progenitors is required for postmitotic neuronal survival and differentiation in the developing cortex (2016)
  • Author(s): Kohei Ohnishi, Noriyuki Sugo, Shunsuke Toyoda, Teruyoshi Hirayama, Takeshi Yagi, Nobuhiko Yamamoto
  • Organizer: The 39th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
  • Int'l Joint Research
• [Presentation] Rho guanine nucleotide exchange factor ARHGEF18 promotes axon branching of upper layer cortical neurons (2016)
  • Author(s): Kensuke Sasaki，Kei Arimoto, Kento Kankawa, Chikayo Terada, Nobuhiko Yamamoto
  • Organizer: The 39th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
  • Int'l Joint Research
• [Presentation] Role of Kit ligand as a negative regulator in activity-dependent thalamocortical axon branching (2016)
  • Author(s): Yumi Miyasaka, Yasufumi Hayano, Takeshi Araki, Nobuhiko Yamamoto
  • Organizer: The 39th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 横浜
  • Year and Date: 2016-07-20
  • Int'l Joint Research
• [Presentation] Polβ deficiency increases DNA double-strand breaks in postnatal cortical neurons (2015)
  • Author(s): Uyeda, A., Onishi, K., Sugo, N., Toyoda S., Hiyayama, T., Yagi, T. & Yamamoto, N.
  • Organizer: 第38回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] DNA polymerase β activity in neural progenitors is required for postmitotic neuronal survival in the developing cortex (2015)
  • Author(s): Onishi, k., Sugo, N., Toyoda, S., Hirayama, T., Yagi, T. & Yamamoto, N.
  • Organizer: Society for Neuroscience
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] Activity-dependent dynamics of CREB in cortical neurons: A single-molecule imaging study (2015)
  • Author(s): Kitagawa, H., Sugo., N. & Yamamoto, N.
  • Organizer: Society for Neuroscience
  • Place of Presentation: Chicago, USA
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] Involvement of mitochondria in thalamocortical axon branching (2015)
  • Author(s): Matsumoto, N., Hoshiko, M. & Yamamoto, N.
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-07-28
• [Presentation] Rho guanine nucleotide exchange factor Abr promotes axon branching in cortical layer 2/3 neurons (2015)
  • Author(s): 41.Sasaki, K., Arimoto, K., Kankawa, K., Terada, C. & Yamamoto, N.
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-07-28
• [Remarks] 大阪大学大学院生命機能研究科 細胞分子神経生物学研究室 （山本亘彦研究室）
  • URL: http://www.fbs.osaka-u.ac.jp/labs/neurobiol/