Mutli-Omics single cell analysis
| Project/Area Number |
15H04318
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Suzuki Yutaka 東京大学, 大学院新領域創成科学研究科, 教授 (40323646)
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| Co-Investigator(Kenkyū-buntansha) |
土原 一哉 国立研究開発法人国立がん研究センター, 先端医療開発センター, 分野長 (00415514)
山下 理宇 東北大学, 東北メディカル・メガバンク機構, 准教授 (10401259)
河野 隆志 国立研究開発法人国立がん研究センター, 研究所, 分野長 (80280783)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | 多層オーミクス解析 / シングルセル解析 / 単一細胞解析 / 次世代シークエンス / 多層オーミクス / 細胞多様性 / ゲノム / がん多様性 / マルチオミクス解析 / ガン細胞 |
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| Outline of Final Research Achievements |
In this study, we have attempted to develop a series of methods by which single cell multi-omics analysis is enabled. We have successfully developed a suite of single cell analytical methods for transcriptional start sites analysis and open chromatin analysis, even though single cell DNA methylation analysis was not enabled mainly due to the difficulties in strict control of the bisulfite reaction. We have applied the developed method to monitor the diverse expression regulations between individual cells. We described fluctuating transcriptional responses invoked by the anti-cancer drugs either in susceptible or resistant lung cancer cell lines. We further examined transcriptome data from clinical specimen using a public cancer genome database and found that similar response seems to also occur in vivo. In addition to applying the developed method for our own purposes, we provided it to a number of researchers via collaborations.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Combinatory use of distinct single-cell RNA-seq analytical platforms reveals the heterogeneous transcriptome response2018
Author(s)
Yukie Kashima, Ayako Suzuki, Ying Liu, Masahito Hosokawa, Hiroko Matsunaga, Masataka Shirai, Kohji Arikawa, Sumio Sugano, Takashi Kohno, Haruko Takeyama, Katsuya Tsuchihara & Yutaka Suzuki
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Journal Title
Scientific Reports
Volume: volume 8
Issue: 1
Pages: 3482-3482
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Presentation] がん細胞の多様性解析2015
Author(s)
鈴木穣
Organizer
日本人類遺伝学会
Place of Presentation
京王プラザホテル（東京都新宿区）
Year and Date
2015-10-15
Related Report
Invited
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