Trial for determining the structure of CXCR7

• Project/Area Number: 15H04338
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyoto University
• Principal Investigator: Shimamura Tatsuro 京都大学, 医学研究科, 特定講師 (90391979)
• Co-Investigator(Renkei-kenkyūsha): SUGA Hiroaki 東京大学, 大学院理学研究科, 教授 (00361668) ; YAMANAKA Yasuaki 京都大学, 大学院医学研究科, 研究員 (10724049) ; NOMURA Norimichi 京都大学, 大学院医学研究科, 助教 (10314246) ; FUJIWARA Takaaki 京都大学, 大学院医学研究科, 研究員 (70712751)
• Research Collaborator: IM Dohyun
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000); Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
• Keywords: 結晶構造解析 / Gタンパク質共役型受容体 / シグナル伝達 / タンパク質 / G蛋白質共役型受容体 / 蛋白質 / 癌

Outline of Final Research Achievements

Chemokine receptor CXCR7 is a member of G protein coupled receptors (GPCR) and a potential drug target. CXCR7 is highly expressed in cancer cells and mediates tumor metastasis. Uniquely, CXCR7 does not couple to G protein but to beta-arrestin. The aim of this project is to determine the 3D structure of CXCR7. Currently, we have not succeeded to obtain crystals of CXCR7.

Research Products

• [Int'l Joint Research] VU University Amsterdam(オランダ)
• [Int'l Joint Research] VU University Amsterdam(オランダ)
• [Journal Article] Crystal Structures of Human Orexin 2 Receptor Bound to the Subtype-Selective Antagonist EMPA (2018)
  • Author(s): Suno Ryoji、Kimura Kanako Terakado、Nakane Takanori、Fujiwara Takaaki、Yamanaka Yasuaki、Im Dohyun、Rosenbaum Daniel M.、Iwata So、Shimamura Tatsuro、Kobayashi Takuya
  • Journal Title: Structure Volume: 26 Issue: 1 Pages: 7-19
  • DOI: 10.1016/j.str.2017.11.005
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A three dimensional movie of structural changes in bacteriorhodopsin. (2016)
  • Author(s): Nango, E., Royant, A.†, Kubo, M.† et al. (†equal contribution)(45名中2番目)
  • Journal Title: Science Volume: 354 Issue: 6319 Pages: 1552-1557
  • DOI: 10.1126/science.aah3497
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Membrane protein structure determination by SAD, SIR, or SIRAS phasing in serial femtosecond crystallography using an iododetergent (2016)
  • Author(s): Takanori Nakane, Shinya Hanashima, Mamoru Suzuki, Haruka Saiki, Taichi Hayashi, Keisuke Kakinouchi, Shigeru Sugiyama, Satoshi Kawatake, Shigeru Matsuoka, Nobuaki Matsumori, et al
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 113 Issue: 46 Pages: 13039-13044
  • DOI: 10.1073/pnas.1602531113
  • Peer Reviewed / Open Access
• [Journal Article] High-resolution crystal structure of the therapeutic antibody pembrolizumab bound to the human PD-1. (2016)
  • Author(s): Horita S, Nomura Y, Sato Y, Shimamura T, Iwata S, Nomura N.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 35297-35297
  • DOI: 10.1038/srep35297
  • NAID: 120005850219
  • Peer Reviewed / Open Access
• [Journal Article] Haem-dependent dimerization of PGRMC1/Sigma-2 receptor facilitates cancer proliferation and chemoresistance. (2016)
  • Author(s): Kabe Y, Nakane T, Koike I, Yamamoto T, Sugiura Y, Harada E, Sugase K, Shimamura T, Ohmura M, Muraoka K, Yamamoto A, Uchida T, Iwata S, Yamaguchi Y, Krayukhina E, Noda M, Handa H, Ishimori K, Uchiyama S, Kobayashi T, Suematsu M.
  • Journal Title: Nature Communications Volume: 7 Issue: 1 Pages: 11030-11030
  • DOI: 10.1038/ncomms11030
  • NAID: 130007529314
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Overview of Membrane Protein Purification and Crystallization (2016)
  • Author(s): Shimamura T.
  • Journal Title: Advanced Methods in Structural Biology Volume: なし
  • Acknowledgement Compliant
• [Journal Article] Native sulfur/chlorine SAD phasing for serial femtosecond crystallography. (2015)
  • Author(s): Nakane T, Song C, Suzuki M, Nango E, Kobayashi J, Masuda T, Inoue S, Mizohata E, Nakatsu T, Tanaka T, Tanaka R, Shimamura T, Tono K, Joti Y, Kameshima T, Hatsui T, Yabashi M, Nureki O, Iwata S, Sugahara M.
  • Journal Title: Acta Crystallogr D Biol Crystallogr. Volume: 71 Issue: 12 Pages: 2519-25
  • DOI: 10.1107/s139900471501857x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Diverse application platform for hard X-ray diffraction in SACLA (DAPHNIS): application to serial protein crystallography using an X-ray free-electron laser. (2015)
  • Author(s): Tono K, Shimamura T, et al.
  • Journal Title: J. Synchrotron Rad. Volume: 22 Issue: 3 Pages: 532-537
  • DOI: 10.1107/s1600577515004464
  • Peer Reviewed
• [Journal Article] Structure and mechanism of the mammalian fructose transporter GLUT5 (2015)
  • Author(s): Nomura N, Verdon G, Kang HJ, Shimamura T, Nomura Y, Sonoda Y, Hussien SA, Qureshi AA, Coincon M, Sato Y, Abe H, Nakada-Nakura Y, Hino T, Arakawa T, Kusano-Arai O, Iwanari H, Murata T, Kobayashi T, Hamakubo T, Kasahara M, Iwata S, Drew D
  • Journal Title: Nature Volume: 526 Issue: 7573 Pages: 397-401
  • DOI: 10.1038/nature14909
  • NAID: 120005657713
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] X線自由電子レーザーを利用したGPCRのシリアルフェムト秒結晶構造解析 (2017)
  • Author(s): 島村達郎
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-26
  • Invited
• [Presentation] A first novel GPCR structure determined at SACLA (2017)
  • Author(s): Dohyun Im, Tatsuro Shimamura, So Iwata
  • Organizer: The International Symposium on Frontiers in structural biology for drug development
  • Int'l Joint Research
• [Presentation] ヒスタミンH1受容体による抗ヒスタミン薬の認識機構 (2016)
  • Author(s): 藤原孝彰，森本志保，山中保明，中根崇智，平田邦生，山下恵太郎，岩田想，島村達郎
  • Organizer: 日本結晶学会2016年度年会
  • Place of Presentation: 水戸
  • Year and Date: 2016-11-17
• [Presentation] X線自由電子レーザーを用いたGPCRの構造解析 (2016)
  • Author(s): 島村達郎
  • Organizer: 第16回日本蛋白質科学会年会
  • Place of Presentation: 博多
  • Year and Date: 2016-06-08
  • Invited
• [Presentation] X線自由電子レーザーを用いたGPCRの構造解析 (2016)
  • Author(s): 島村達郎
  • Organizer: 日本蛋白質科学会年会
  • Place of Presentation: 福岡国際会議場（福岡市）
  • Year and Date: 2016-06-08
  • Invited