NMR approach for the functional structural analyses of human membrane proteins
| Project/Area Number |
15H04340
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | Yokohama City University |
|---|
Principal Investigator |
Takahashi Hideo 横浜市立大学, 生命医科学研究科, 教授 (60265717)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
竹内 恒 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究チーム長 (20581284)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SAKAKURA Masayoshi 横浜市立大学, 大学院生命医科学研究科, 助教 (20334336)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
|
|---|
| Keywords | NMR / 膜タンパク質 / 酵母 |
|---|
| Outline of Final Research Achievements |
In this study, we have developed a novel and cost-effective strategy for P. pastoris expression systems to selectively incorporate 13C into methyl groups of Val and Leu by using the alpha-ketoacid precursor. Methyl-specific isotopically-labeled samples are quite pivotal for investigating the structures and interactions of large macromolecules such as membrane proteins by solution NMR. We further established an experimental procedure to prepare NLP-reconstituted human membrane protein (C99) which allows analyzing membrane proteins in lipid bilayer membranes by solution NMR. Our NMR analysis revealed that the lipid composition in the bilayer remarkably influences structure and dynamics of the transmembrane helix region.
|
Report
(4 results)
Research Products
(37 results)
-
-
-
[Journal Article] 15N detection harnesses the slow relaxation property of nitrogen: Delivering enhanced resolution for intrinsically disordered proteins.2018
Author(s)
Chhabra S, Fischer P, Takeuchi K, Dubey A, Ziarek JJ, Boeszoermenyi A, Mathieu D, Bermel W, Davey NE, Wagner G, Arthanari H.
-
Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 115
Issue: 8
Pages: 1710-1719
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
