| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
|
|---|
| Outline of Final Research Achievements |
Gene transcription is a complex, multi-step process consisting of transcriptional initiation, elongation, and termination. RNA polymerase (RNAP) alone cannot accomplish transcription, and, in each step, a number of transcription factors bind RNAP to support its function. In this study, we analyzed the structures of RNAP bound with bacteriophage transcription factors and ribosome, which is involved in the prokaryotic transcription regulation. We also succeeded in structure determination of a huge transcription elongation complex formed in the eukaryotic nucleus, and first elucidated the mechanism by which the elongation complex transcribes nucleosomal DNA.
|
