Investigation for novel circadian clock reactions independent of the transcription-translation feedback loop of clock genes
| Project/Area Number |
15H04349
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
Take Ohkawa 名古屋大学, 生命農学研究科, 准教授 (30432230)
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| Co-Investigator(Kenkyū-buntansha) |
望田 啓子 (桑田啓子) 名古屋大学, トランスフォーマティブ生命分子研究所, 特任助教 (70624352)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Keywords | 概日時計 / ケミカルバイオロジー / 翻訳後修飾 / 概日リズム / プロテオミクス / プロテオーム / 蛋白質 / 質量分析 / 自律振動 |
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| Outline of Final Research Achievements |
The widely accepted models for circadian clocks are based on transcriptional-translational negative feedback loops composed of circadian clock genes. However, some important aspects, such as how the periods are adjusted around 24 h and how the phase of the rhythms are determined, are not fully incorporated into these models. On the other hand, there are several examples of protein posttranscriptional modification rhythms independent of the feedback loops, though their exact roles in the circadian clock systems are still unknown. In this study we tried to elucidate these points by proteomic and chemical biology approach. We obtained compounds that affect period and phase and the potential candidates of their target proteins. We also set up the system to study posttranslational modification rhythms including phosphorylation. By these bidirectional approaches, we are now trying to understand the whole circadian clock systems.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Identification of circadian clock modulators from existing drugs2018
Author(s)
T. Katherine Tamai, Yusuke Nakane, Wataru Ota, Akane Kobayashi, Masateru Ishiguro, Naoya Kadofusa, Keisuke Ikegami, Kazuhiro Yagita, Yasufumi Shigeyoshi, Masaki Sudo, Taeko Nishiwaki-Ohkawa, Ayato Sato, & Takashi Yoshimura
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Journal Title
EMBO Molecular Medicine
Volume: -
Issue: 5
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Combination of synthetic chemistry and live-cell imaging identified a rapid cell division inhibitor in tobacco and Arabidopsis thaliana2016
Author(s)
Nambo, M., Kurihara, D., Yamada, T., Nishiwaki-Ohkawa, T., Kadofusa, N., Kimata, Y., Kuwata, K., Umeda, M. and Ueda, M.
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Journal Title
Plant Cell Physiol.
Volume: 57
Issue: 11
Pages: 2255-2268
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Phosphorylation of the C Terminus of RHD3 Has a Critical Role in Homotypic ER Membrane Fusion in Arabidopsis.2016
Author(s)
Ueda, H., Yokota, E., Kuwata, K., Kutsuna, N., Mano, S., Shimada, T., Tamura, K., Stefano, G., Fukao, Y., Brandizzi, F., Shimmen, T., Nishimura, M., Hara-Nishimura, I.
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Journal Title
Plant Physiol.
Volume: 170
Issue: 2
Pages: 867-880
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] C-H activation generates period-shortening molecules that target crptochrome in the mammalian circadian clock2015
Author(s)
Tsuyoshi Oshima, Iori Yamanaka, Anupriya Kumar, Junichiro Yamaguchi, Taeko Nishiwaki-Ohkawa, Kei Muto, Rika Kawamura, Tsuyoshi Hirota, Kazuhiro Yagita, Stephan Irle, Steve A. Kay Takashi Yoshimura, Kenichiro Itami
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Journal Title
Angewandte Chemie International Edition
Volume: 54
Pages: 7193-7197
Related Report
Peer Reviewed
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