New Frontier of Photosynthesis Research by Using the Cryogenic Optical Microscope
| Project/Area Number |
15H04356
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
野口 巧 名古屋大学, 理学研究科, 教授 (60241246)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
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| Keywords | 共焦点顕微鏡 / 極低温 / 単一分子分光 / ステート遷移 / 光合成 / 励起スペクトル / 顕微分光 / 偏光異方性 |
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| Outline of Final Research Achievements |
By using the developed cryogenic microscope system, intracellular inhomogeneous distributions of photosynthetic proteins of Chlamydomonas, a model organism for the photosynthesis study, were observed. The analysis clarified the extent of co-localizations of photosystem proteins and light-harvesting antenna proteins. This results demonstrated that antenna proteins actually shuttles between the two photosystem proteins upon state transition, which is a regulation mechanism of excitation balance of the two photosystems.
A system enabling scanning of the excitation wavelength was developed. By using the system, the fluorescence excitation spectra of single photosynthesis proteins were obtained for the first time. Information about the excitation-energy flow within the protein was obtained.
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Report
(4 results)
Research Products
(33 results)
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[Presentation] タンパク質中金ナノクラスターの生成過程および発光特性2017
Author(s)
出澤 拓磨, Hamza Al-kindi, Izabela Rzeznicka, 福村 裕史, 柴田 穣
Organizer
Advanced Light Measurements and Life Sciences Seeking Bio. Phys. Chem. about the Triple Point, 最先端光計測とライフサイエンスの近未来 －Bio. Phys. Chem.三重点の探索
Related Report
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