Elucidation of translational regulatory mechanism by RNA-protein complex
| Project/Area Number |
15H04358
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Funatsu Takashi 東京大学, 大学院薬学系研究科(薬学部), 教授 (00190124)
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| Co-Investigator(Renkei-kenkyūsha) |
OKABE Kohki 東京大学, 大学院薬学系研究科, 助教 (20455398)
IIZUKA Ryo 東京大学, 大学院薬学系研究科, 助教 (90541954)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Keywords | １分子計測（SMD） / ナノバイオ / 蛋白質 / １分子計測（SMD) / 光ピンセット / リボソーム / １分子生理学・生化学 / １分子イメージング / ナノ計測 / １分子計測(SMD) |
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| Outline of Final Research Achievements |
1. Fluorescently labeled miRNA precursors were microinjected into a cell. They were matured by endogenous biosynthetic pathway and they were incorporated into RISC. Single-molecule imaging technique revealed that miRNAs move on actin filaments.
2. Localization of mRNAs labeled with Cy5-linear antisense DNA probe was investigated under the stress conditions. Super-resolution microcopy revealed that mRNAs are not uniformly present in the stress granule (SG) but are accumulated to form high density regions with a diameter of about 100 nm.
3. The lifetimes of translational arrest of SecM mutants, whose amino acids outside ribosome were partially truncated, were investigated. Amino acid residues 57-73 and 57-98 contributed to the stabilization of translational arrest.
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Report
(4 results)
Research Products
(46 results)
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[Presentation] Culture-Independent Method for Identification of Microbial Enzyme-Encoding Genes by Single-Cell Sequencing Using a Water-in-oil Microdroplet Platform2017
Author(s)
Kazuki Nakamura, Ryo Iizuka, Shinro Nishi, Takao Yoshida, Yuji Hatada, Yoshihiro Takaki, Ayaka Iguchi, Dong H. Yoon, Tetsushi Sekiguchi, Shuichi Shoji, TakashiFunatsu
Organizer
Single-Cell Biophysics: Measurement, Modulation, and Modeling
Related Report
Int'l Joint Research
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