Revisiting "the multi-tubulin hypothesis" through comprehensive analysis of tubulin isotypes
| Project/Area Number |
15H04369
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Tohoku University |
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Principal Investigator |
Sugimoto Asako 東北大学, 生命科学研究科, 教授 (80281715)
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| Co-Investigator(Renkei-kenkyūsha) |
KUBOTA Yukihiko 東北大学, 大学院生命科学研究科, 助教 (70333325)
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| Research Collaborator |
HARUTA Nami 東北大学, 大学院生命科学研究科, 博士研究員
HONDA Yu 東北大学, 大学院生命科学研究科, 大学院生
TSUCHIYA Kenta 東北大学, 大学院生命科学研究科, 大学院生
OBINATA Hiroyuki 東北大学, 大学院生命科学研究科, 大学院生
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Keywords | 微小管 / チューブリンアイソタイプ / 線虫 / 細胞・組織 / チューブリン / C.elegans |
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| Outline of Final Research Achievements |
In this study, we tested the "multi-tubulin hypothesis" in which multiple tubulin isotypes contribute to the diversity of microtubule dynamics, using the nematode C. elegans as a model system. Using genome editing, all tubulin isotype genes (9 alpha and 6 beta genes) were systematically knocked out and tagged with GFP, and their expression patterns and loss-of-function phenotypes were analyzed. Each isotypes were expressed in specific cell types, and ectopic expression experiments proved that composition of tubulin isotypes contribute to the diversity of microtubule dynamics.
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Report
(4 results)
Research Products
(15 results)
