Elucidating the mechanisms underlying epithelial cell height change mediated by modifications of apical-basal polarity
| Project/Area Number |
15H04373
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cell biology
|
|---|
| Research Institution | Institute of Physical and Chemical Research |
|---|
Principal Investigator |
Wang Yuchiun 国立研究開発法人理化学研究所, 多細胞システム形成研究センター, チームリーダー (80725995)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
|
|---|
| Keywords | Epithelial folding / Apical-basal polarity / Microtubule mechanics / microtubule forces / Patronin/CAMSAP / cell shortening / size homeostasis / apical-basal polarity / microtubule network / cell shape control / epithelial folding / epithelial cell height / CAMSAP/Patronin / microtubule mechanics / microtubule homeostasis / cell shape change / Epithelial cell height |
|---|
| Outline of Final Research Achievements |
In the Drosophila gastrula, dorsal fold formation occurs despite a lack of localized myosin changes, while the fold-initiating cells reduce cell height following basal shifts of polarity via an unknown mechanism. We show that cell shortening that initiate dorsal fold formation depends on an apical microtubule network that is organized by the CAMSAP protein Patronin. Prior to gastrulation, microtubule forces generated by the minus-end motor dynein scaffold the apical cell cortex into a dome-like shape, while the severing enzyme Katanin facilitates network remodelling to ensure tissue-wide cell size homeostasis. During fold initiation, Patronin redistributes following basal polarity shifts in the initiating cells, apparently weakening the scaffolding forces to allow dome descent. The homeostatic network that ensures size/shape homogeneity is thus repurposed for cell shortening, mechanistic linking epithelial polarity and folding via a microtubule-based mechanical mechanism.
|
Report
(4 results)
Research Products
(7 results)
