Analysis of environmental sex determination

• Project/Area Number: 15H04396
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Yokohama City University
• Principal Investigator: Iguchi Taisen 横浜市立大学, 生命ナノシステム科学研究科(八景キャンパス), 特任教授 (90128588)
• Co-Investigator(Kenkyū-buntansha): 荻野 由紀子 九州大学, 農学研究院, 准教授 (00404343) ; 宮川 信一 和歌山県立医科大学, 先端医学研究所, 講師 (30404354)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2015: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
• Keywords: 性決定 / 環境 / 爬虫類 / 甲殻類 / 温度 / ミジンコ / ホルモン / 発生・分化

Outline of Final Research Achievements

Sex determination is a critical element in animal development and evolution. In contrast to sex determination based on intrinsic genotypic factors, as commonly seen in many animals, some species show environmental sex determination (ESD), where offspring become male or female in response to an environmental factor(s) during development. We investigated ESD mechanism using American alligator and water flea (daphnia) as a models. In alligator, we identified thermosensitive transient receptor potential (TRP) channels as main initiation candidate. In addition, we revealed that epigenomic factors might be involved in temperature-dependent sex determination. In Daphnids, we identified several juvenile hormone and ecdysone biosynthesis-related genes and investigated their expression levels throughout embryogenesis and molting cycle between male and female. These results provide novel insights towards understanding photoperiod-dependent sex determination process in Daphnids.

Research Products

• [Int'l Joint Research] Norwegian Institute for Water Research/The Arctic University of Norway(ノルウェー)
• [Int'l Joint Research] US Environmental Protection Agency,/Medical University of South Carolina/Badger Technical Services(米国)
• [Int'l Joint Research] University of Exeter/University of Birmingham(英国)
• [Int'l Joint Research] Medical University of South Carolina/Kennedy Space Center(米国)
• [Int'l Joint Research] University of Stellenbosch(南アフリカ)
• [Int'l Joint Research] University of Birmingham(英国)
• [Int'l Joint Research] Norwegian Institute for Water Research(ノルウェー)
• [Int'l Joint Research] Medical University of South Carolina(米国)
• [Int'l Joint Research] University of Birmingham(英国)
• [Int'l Joint Research] Norwegian Institute for Water Research(ノルウェー)
• [Journal Article] Juvenile hormone-independent function of Kruppel homolog 1 in early development of water flea Daphnia pulex (2018)
  • Author(s): Miyakawa Hitoshi、Watanabe Minae、Araki Marina、Ogino Yukiko、Miyagawa Shinichi、Iguchi Taisen
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: 93 Pages: 12-18
  • DOI: 10.1016/j.ibmb.2017.12.007
  • Peer Reviewed
• [Journal Article] Effects of 2,3-Bis(4-hydroxyphenyl)-propionitrile on Induction of Polyovular Follicles in the Mouse Ovary (2018)
  • Author(s): Sato Tomomi、Kim Hannah、Kakuta Hanako、Iguvhi Taisen
  • Journal Title: In Vivo Volume: 32 Issue: 1 Pages: 19-24
  • DOI: 10.21873/invivo.11199
  • Peer Reviewed / Open Access
• [Journal Article] Identification of hepatic thyroid hormone-responsive genes in neonatal rats: Potential targets for thyroid hormone-disrupting chemicals. (2018)
  • Author(s): Fujimoto N., Kitamura S., Uramaru N., Miyagawa S., Iguchi T.
  • Journal Title: Toxicology Letters Volume: 286 Pages: 48-53
  • DOI: 10.1016/j.toxlet.2018.01.005
  • Peer Reviewed / Open Access
• [Journal Article] Effects of tributyltin on early life-stage, reproduction, and gonadal sex differentiation in Japanese medaka (Oryzias latipes). (2018)
  • Author(s): Horie Y, Yamagishi T, Shintaku Y, Iguchi T, Tatarazako N.
  • Journal Title: Chemosphere Volume: 203 Pages: 418-425
  • DOI: 10.1016/j.chemosphere.2018.03.135
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Toxic effects of chemical dispersant Corexit 9500 on water flea Daphnia magna (2017)
  • Author(s): Toyota K, McNabb NA, Spyropoulos DD, Iguchi T, Kohno S
  • Journal Title: J Appl Toxicol Volume: 37 Issue: 2 Pages: 201-206
  • DOI: 10.1002/jat.3343
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Development of anin vivoanti-androgenic activity detection assay using fenitrothion in Japanese medaka (Oryzias latipes) (2017)
  • Author(s): Horie Yoshifumi、Watanabe Haruna、Takanobu Hitomi、Yagi Ayano、Yamagishi Takahiro、Iguchi Taisen、Tatarazako Norihisa
  • Journal Title: Journal of Applied Toxicology Volume: 37 Issue: 3 Pages: 339-346
  • DOI: 10.1002/jat.3365
  • Peer Reviewed
• [Journal Article] omparative ovarian microarray analysis of juvenile hormone-responsive genes in water flea Daphnia magna: potential targets for toxicity (2017)
  • Author(s): Toyota K, Williams TD, Sato T, Tatarazako N, Iguchi T
  • Journal Title: J Appl Toxicol Volume: 37 Issue: 3 Pages: 374-381
  • DOI: 10.1002/jat.3368
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Protein kinase C involved with upstream signaling of mehyl farnesoate for the photoperiod-dependent sex determination in the water flea Daphnia pulex (2017)
  • Author(s): Toyota Kenji、Sato Tomomi、Tatarazako Norihisa、Iguchi Taisen
  • Journal Title: Biology Open Volume: 6 Pages: 161-164
  • Peer Reviewed / Open Access
• [Journal Article] Development of a common carp (Cyprinus carpio) pregnane X receptor (cPXR) transactivation reporter assay and its activation by azole fungicides and pharmaceutical chemicals. (2017)
  • Author(s): Lange A, Corcorana J, Miyagawa S, Iguchi T, Winter MJ, Tyler CR.
  • Journal Title: Toxicol In Vitro Volume: 41 Pages: 114-122
  • DOI: 10.1016/j.tiv.2017.02.023
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Lethal and sublethal effects of aniline and chlorinated anilines on zebrafish embryos and larvae (2017)
  • Author(s): Horie Yoshifumi、Yamagishi Takahiro、Koshio Masaaki、Iguchi Taisen、Tatarazako Norihisa
  • Journal Title: Journal of Applied Toxicology Volume: 37 Issue: 7 Pages: 836-841
  • DOI: 10.1002/jat.3431
  • Peer Reviewed
• [Journal Article] Comparative luciferase assay for establishing reliable in vitro screening system of juvenile hormone agonists (2017)
  • Author(s): Miyakawa Hitoshi、Iguchi Taisen
  • Journal Title: Journal of Applied Toxicology Volume: 37 Issue: 9 Pages: 1082-1090
  • DOI: 10.1002/jat.3459
  • Peer Reviewed
• [Journal Article] Altered expression of Olr59, Ethe1, and Slc10a2 genes in the liver of F344 rats by neonatal thyroid hormone disruption. (2017)
  • Author(s): Matsubara K et al.
  • Journal Title: J Appl Toxicol Volume: - Issue: 9 Pages: 1030-1035
  • DOI: 10.1002/jat.3452
  • Peer Reviewed / Open Access
• [Journal Article] Photoperiodism of Male Offspring Production in the Water Flea Daphnia pulex (2017)
  • Author(s): Toyota Kenji、Sato Tomomi、Tatarazako Norihisa、Iguchi Taisen
  • Journal Title: Zoological Science Volume: 34 Issue: 4 Pages: 312-317
  • DOI: 10.2108/zs170005
  • NAID: 40021289675
  • Peer Reviewed
• [Journal Article] Assessment of the lethal and sublethal effects of 20 environmental chemicals in zebrafish embryos and larvae by using OECD TG 212 (2017)
  • Author(s): Horie Yoshifumi、Yamagishi Takahiro、Takahashi Hiroko、Shintaku Youko、Iguchi Taisen、Tatarazako Norihisa
  • Journal Title: Journal of Applied Toxicology Volume: 37 Issue: 10 Pages: 1245-1253
  • DOI: 10.1002/jat.3487
  • Peer Reviewed
• [Journal Article] Establishment of estrogen receptor 1 (ESR1)-knockout medaka; ESR1 is dispensable for sexual development and reproduction in medaka, Oryzias latipes. (2017)
  • Author(s): Tohyama S, Ogino Y, Lange A, Myosho T, Kobayashi T, Hirano Y, Yamada G, Sato T, Tatarazako N, Tyler CR, Iguchi T, Miyagawa S.
  • Journal Title: Dev Growth Diff Volume: 59 Issue: 6 Pages: 552-561
  • DOI: 10.1111/dgd.12386
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Estrogen alters gonadal soma-derived factor ( Gsdf)/Foxl2 expression levels in the testes associated with testis-ova differentiation in adult medaka, Oryzias latipes (2017)
  • Author(s): Kobayashi Tohru、Chiba Ayaka、Sato Tadashi、Myosho Taijun、Yamamoto Jun、Okamura Tetsuro、Onishi Yuta、Sakaizumi Mitsuru、Hamaguchi Satoshi、Iguchi Taisen、Horie Yoshifumi
  • Journal Title: Aquatic Toxicology Volume: 191 Pages: 209-218
  • DOI: 10.1016/j.aquatox.2017.08.011
  • Peer Reviewed
• [Journal Article] Effects of triphenyltin on reproduction in Japanese medaka ( Oryzias latipes ) across two generations (2017)
  • Author(s): Horie Yoshifumi、Watanabe Haruna、Takanobu Hitomi、Shigemoto Yoshiko、Yamagishi Takahiro、Iguchi Taisen、Tatarazako Norihisa
  • Journal Title: Aquatic Toxicology Volume: 192 Pages: 16-23
  • DOI: 10.1016/j.aquatox.2017.08.016
  • Peer Reviewed
• [Journal Article] Medaka extended one-generation reproduction test evaluating 4-nonylphenol (2017)
  • Author(s): Watanabe Haruna、Horie Yoshifumi、Takanobu Hitomi、Koshio Masaaki、Flynn Kevin、Iguchi Taisen、Tatarazako Norihisa
  • Journal Title: Environmental Toxicology and Chemistry Volume: 36 Issue: 12 Pages: 3254-3266
  • DOI: 10.1002/etc.3895
  • Peer Reviewed
• [Journal Article] Summary of the development the US Environmental Protection Agency's Medaka Extended One Generation Reproduction Test (MEOGRT) using data from 9 multigenerational medaka tests (2017)
  • Author(s): Flynn Kevin、Lothenbach Doug、Whiteman Frank、Hammermeister Dean、Touart Leslie W.、Swintek Joe、Tatarazako Norihisa、Onishi Yuta、Iguchi Taisen、Johnson Rodney
  • Journal Title: Environmental Toxicology and Chemistry Volume: 36 Issue: 12 Pages: 3387-3403
  • DOI: 10.1002/etc.3923
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Release of chitobiase as an indicator of potential molting disruption in juvenile Daphnia magna exposed to the ecdysone receptor agonist 20-hydroxyecdysone (2017)
  • Author(s): Song You、Evenseth Linn Mari、Iguchi Taisen、Tollefsen Knut Erik
  • Journal Title: Journal of Toxicology and Environmental Health, Part A Volume: 80 Issue: 16-18 Pages: 954-962
  • DOI: 10.1080/15287394.2017.1352215
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effects of triclosan on Japanese medaka (Oryzias latipes) during embryo development, early life stage and reproduction (2017)
  • Author(s): Horie Yoshifumi、Yamagishi Takahiro、Takahashi Hiroko、Iguchi Taisen、Tatarazako Norihisa
  • Journal Title: Journal of Applied Toxicology Volume: 38 Issue: 4 Pages: 544-551
  • DOI: 10.1002/jat.3561
  • Peer Reviewed
• [Journal Article] rotein kinase C is involved with upstream signaling of methyl farnesoate for photoperiod-dependent sex determination in the water flea Daphnia pulex (2017)
  • Author(s): Toyota K, Sato T, Tatarazako N, Iguchi T
  • Journal Title: Biol Open Volume: 15 Pages: 161-164
  • DOI: 10.1242/bio.021857
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] RNA-seq analysis of the gonadal transcriptome during Alligator mississippiensis temperature-dependent sex determination and differentiation. (2016)
  • Author(s): Yatsu R, Miyagawa S, Kohno S, Parrott BB, Yamaguchi K, Ogino Y, Miyakawa H, Lowers RH, Shigenobu S, Guillette LJ Jr, Iguchi T.
  • Journal Title: BMC Genomics Volume: 17 Issue: 1 Pages: 77-77
  • DOI: 10.1186/s12864-016-2396-9
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Characterization of evolutionary trend in squamate estrogen receptor sensitivity (2016)
  • Author(s): Yatsu R, Katsu Y, Kohno S, Mizutani T, Ogino Y, Ohta Y, Myburgh J, van Wyk JH, Guillette Jr LJ, Miyagawa S, Iguchi T.
  • Journal Title: Gen Comp Endocrinol Volume: 238 Pages: 88-95
  • DOI: 10.1016/j.ygcen.2016.04.005
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Metabolomics reveals an involvement of pantothenate for male production responding to the short-day stimulus in the water flea, Daphnia pulex (2016)
  • Author(s): Toyota K, Gavin A, Miyagawa S, Viant MR, Iguchi T
  • Journal Title: Sci Rep Volume: 2016 Issue: 1 Pages: 25125-25125
  • DOI: 10.1038/srep25125
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Neverland regulates embryonic molts through the regulation of ecdysteroids synthesis in the water flea Daphnia magna (2016)
  • Author(s): Sumiya E, Ogino Y, Toyota K, Miyakawa H, Miyagawa S, Iguchi T
  • Journal Title: J Appl Toxicol Volume: 36 Pages: 1476-1485
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Whole-organism transcriptomic analysis provides mechanistic insight into the acute toxicity of emamectin benzoate in Daphnia magna (2016)
  • Author(s): Song Y, Rundberget JT, Evenseth LM, Xie L, Gomes T, Hogasen T, Iguchi T, Tollefsen KE
  • Journal Title: Environ Sci Technol Volume: 50 Issue: 21 Pages: 11994-12003
  • DOI: 10.1021/acs.est.6b03456
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Molecular cloning and characterization of the aryl hydrocarbon receptors and aryl hydrocarbon receptor nuclear translocators in the American alligator (2016)
  • Author(s): Oka K, Kohno S, Ohta Y, Guillette LJ Jr, Iguchi T, Katsu Y
  • Journal Title: Gen Comp Endocrinol Volume: 238 Pages: 13-22
  • DOI: 10.1016/j.ygcen.2016.05.002
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] TRPV4 associates environmental temprerature and sex determination in the American alligator (2015)
  • Author(s): Yatsu R, Miyagawa S, Kohno S, Saito S, Ogino Y, Katsu Y, Ohta Y, Tominaga M, Guillette LJ, Iguchi T
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 18581-18581
  • DOI: 10.1038/srep18581
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Repeated colonization leads to intra-specific variations in reaction norms of predator-induced polyphenism in the water flea Daphnia pulex (2015)
  • Author(s): Miyakawa H, Sugimoto N, Kohyama TI, Iguchi T, Miura T
  • Journal Title: Ecol Res Volume: 30 Pages: 705-713
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Allosteric role of the amino-terminal A/B domain on corticosteroid transactivation of gar and human glucocorticoid receptors. (2015)
  • Author(s): Oka K, Hoang A, Okada D, Iguchi T, Baker ME, Katsu Y
  • Journal Title: J Steroid Biochem Mol Biol Volume: 154 Pages: 112-119
  • DOI: 10.1016/j.jsbmb.2015.07.025
  • NAID: 120005853068
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Methyl farnesoate synthesis is necessary for the environmental sex determination in water flea Daphnia pulex (2015)
  • Author(s): Kenji Toyota, Hitoshi Miyakawa, Chizue Hiruta, Kenjiro Furuta, Yukiko Ogino, Tetsuro Shinoda, Norihisa Tatarazako, Shinichi Miyagawa, Joseph R. Shaw, Taisen Iguchi
  • Journal Title: Journal of Insect Physiology Volume: - Pages: 22-30
  • DOI: 10.1016/j.jinsphys.2015.02.002
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification and characterization of the androgen receptor from the American alligator, Alligator mississippiensis (2015)
  • Author(s): Miyagawa S, Yatsu R, Kohno S, Doheny BM, Ogino Y, Ishibashi H, Katsu Y, Ohta Y, Guillette LJ Jr, Iguchi T
  • Journal Title: Endocrinology Volume: 156 Issue: 8 Pages: 2795-2806
  • DOI: 10.1210/en.2015-1037
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Estrogen receptor 1 (ESR1; ERα), not ESR2 (ERβ), modulates estrogen-induced sex reversal in the American alligator, a species with temperature-dependent sex determination (2015)
  • Author(s): Kohno, S., Bernhard, M.C., Katsu, Y., Zhu, J., Byan, T.A., Doheny, B.M., Iguchi, T. and Guillette, L.J.Jr.
  • Journal Title: Endocrinology Volume: 印刷中 Issue: 5 Pages: 1887-1899
  • DOI: 10.1210/en.2014-1852
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Ionotropic glutamate receptors mediate inducible defense in the water flea Daphnia pulex (2015)
  • Author(s): Miyakawa, H., Sato, T., Colbourne, J.K. and Iguchi, T.
  • Journal Title: PLoS ONE Volume: 10 Issue: 3 Pages: e0121324-e0121324
  • DOI: 10.1371/journal.pone.0121324
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] NMDA receptor activation upstream of methyl farnesoate signaling for short day-induced male offspring production in the water flea, Daphnia pulex (2015)
  • Author(s): Kenji Toyota, Hitoshi Miyakawa, Katsushi Yamaguchi, Shuji Shigenobu, Yukiko Ogino, Norihisa Tatarazako, Shinichi Miyagawa, Taisen Iguchi
  • Journal Title: BMC Genomics Volume: 16 Issue: 1 Pages: 186-186
  • DOI: 10.1186/s12864-015-1392-9
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Diofenolan induces male offspring production through binding to juvenile hormone receptor in Daphnia magna (2015)
  • Author(s): Ryoko Abe, Kenji Toyota, Hitoshi Miyakawa, Haruna Watanabe, Tomohiro Oka, Shinichi Miyagawa, Hiroyo Nishide, Ikuo Uchiyama, Knut Erik Tollefsen, Taisen Iguchi, Norihisa Tatarazako
  • Journal Title: Aquatic Toxicology Volume: 159 Pages: 44-51
  • DOI: 10.1016/j.aquatox.2014.11.015
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Establishment of a short-term in vivo screening method for detecting chemicals having juvenile hormone activity using adult Daphnia magna (2014)
  • Author(s): R. Abe, H. Watanabe, M. Yamamuro, T. Iguchi and N. Tatarazako
  • Journal Title: J. Appl. Toxicol Volume: 印刷中 Issue: 1 Pages: 75-82
  • DOI: 10.1002/jat.2989
  • Peer Reviewed / Open Access
• [Presentation] 時系列トランスクリプトームから迫るミジンコの環境依存型性決定の分子機構 (2017)
  • Author(s): 豊田賢治、John K Colbourne、井口泰泉、宮川信一
  • Organizer: 日本比較内分泌学会第42回大会
• [Presentation] 温度環境に依存する爬虫類の性決定システム (2017)
  • Author(s): 宮川信一、豊田賢治、谷津遼平、井口泰泉
  • Organizer: 日本比較内分泌学会第42回大会
• [Presentation] エストロゲン受容体1（Esr1/ERα）ノックアウトメダカの表現型解析 (2017)
  • Author(s): 遠山早紀、荻野由紀子、明正大純、佐藤友美、小林亨、井口泰泉、宮川信一
  • Organizer: 日本比較内分泌学会第42回大会
• [Presentation] Time-series transcriptome approach for deciphering the male sex determining mechanisms via juvenile hormone in the water flea Daphnia pulex. (2017)
  • Author(s): Toyota K, Iguchi T, Yamada G, Miyagawa S, Colbourne JK
  • Organizer: 日本内分泌攪乱化学物質学会第20回研究発表会
• [Presentation] メダカエストロゲン受容体（Esr1）の生殖に対する機能解析 (2017)
  • Author(s): 遠山早紀、荻野由紀子、明正大純、佐藤友美、小林亨、井口泰泉、宮川信一
  • Organizer: 日本内分泌攪乱化学物質学会第20回研究発表会
• [Presentation] メダカPPARγのクローニングと代謝異常に関する解析 (2017)
  • Author(s): 遠山早紀、明正大純、佐藤友美、井口泰泉、小林亨、宮川信一
  • Organizer: 日本動物学会第76回大会
• [Presentation] ウス膣上皮細胞増殖と分化に関するエストロゲン受容体の機能解析 (2017)
  • Author(s): 山田源、平野優、井口泰泉、宮川信一
  • Organizer: 第90回日本内分泌学会学術総会
• [Presentation] ミジンコの幼若ホルモンかく乱によるオス仔虫誘導に係るAdverse Outcome Pathwayの構築 (2016)
  • Author(s): 渡部春菜、阿部良子、宮川一志、豊田賢治、井口泰泉、鑪迫典久
  • Organizer: 日本内分泌攪乱化学物質学会第19回研究発表会
  • Place of Presentation: 研究交流センター（茨城県つくば市）
  • Year and Date: 2016-12-08
• [Presentation] Outcomes of the Japanese program on endocrine disruption (EXTEND2010) and new program of endocrine disrupting chemicals in the Japanese Ministry of the Environment (EXTEND2016) (2016)
  • Author(s): Iguchi T
  • Organizer: 7th International Fresenius Conference Endocrine Disruptors
  • Place of Presentation: ボン（ドイツ）
  • Year and Date: 2016-11-15
  • Int'l Joint Research / Invited
• [Presentation] Molecular mechanisms underlying temperature-dependent sex determination in Alligator mississippiensis (2016)
  • Author(s): Miyagawa S.
  • Organizer: 22nd International Conference of Zoology及び日本動物学会第75回大会合同大会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-11-04
  • Int'l Joint Research / Invited
• [Presentation] EDC’s and steroid receptors across species (2016)
  • Author(s): Iguchi T
  • Organizer: 25 Years of Endocrine Disruption Research: Past Lessons and Future Directions.
  • Place of Presentation: ベセダ（アメリカ）
  • Year and Date: 2016-09-18
  • Int'l Joint Research / Invited
• [Presentation] Approach to establishing a knock-in system by using TAL-PITCh in the water flea Daphnia pulex (2016)
  • Author(s): Hiruta C, Sakuma T, Ogino Y, Yamamoto T, Iguchi T
  • Organizer: 日本ゲノム編集学会第1回大会
  • Place of Presentation: 広島国際会議場（広島県広島市）
  • Year and Date: 2016-09-06
• [Presentation] ミジンコとワニの環境依存性性分化について (2016)
  • Author(s): 井口泰泉
  • Organizer: 第9回生殖細胞の会
  • Place of Presentation: 大泉ステーション（山梨県北杜市）
  • Year and Date: 2016-07-30
  • Invited
• [Presentation] Applied toxicity tests for endocrine disrupters (2016)
  • Author(s): Iguchi T, Ogino Y, Miyagawa S, Yatsu R, Tatarazako N
  • Organizer: International Meeting on Aquatic Model Organisms for Human Disease and Toxicology Research
  • Place of Presentation: 岡崎カンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2016-03-18
  • Int'l Joint Research / Invited
• [Presentation] Research on the mechanisms of temperature-dependent sex determination in American alligator, Alligator mississippiensis (2016)
  • Author(s): Miyagawa S, Yatsu R, Kohno S, Guillette Jr LJ, Iguchi T
  • Organizer: The Biology and Ecotoxicology of the American Alligator, The 3rd Biennial Symposium
  • Place of Presentation: タイタスビル（アメリカ）
  • Int'l Joint Research
• [Presentation] Identification of genes involved with juvenile hormone signaling pathways governing environmental sex determination in the water flea, Daphnia pulex (2015)
  • Author(s): Toyota K, Miyakawa H, Yamaguchi K, Shigenobu S, Ogino Y, Miyagawa S, Iguchi T.
  • Organizer: 日本比較内分泌学会第40回大会
  • Place of Presentation: JMSアステールプラザ（広島県広島市）
  • Year and Date: 2015-12-11
• [Presentation] 幼若ホルモン活性物質の新規評価法の確立に向けたミジンコにおける幼若ホルモン応答遺伝子の網羅的探索 (2015)
  • Author(s): 豊田賢治、宮川一志、重信秀治、荻野由紀子、宮川信一、井口泰泉
  • Organizer: 日本内分泌攪乱化学物質学会第18回研究発表会
  • Place of Presentation: 自治医科大学（栃木県下野市）
  • Year and Date: 2015-12-10
• [Presentation] ミジンコの光周期依存的な周期性単為生殖を担う分子基盤の解析 (2015)
  • Author(s): 豊田賢治、井口泰泉
  • Organizer: 2015年度日本時間生物学会
  • Place of Presentation: 東京大学（東京都文京区）
  • Year and Date: 2015-11-21
• [Presentation] ワニの温度依存型性決定メカニズム (2015)
  • Author(s): 宮川信一、谷津遼平、井口泰泉
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2015-09-17
  • Invited
• [Presentation] トランスクリプトーム解析によるミジンコの環境依存型性決定に関与する遺伝子の探索 (2015)
  • Author(s): 豊田賢治、宮川一志、山口勝司、重信秀治、荻野由紀子、宮川信一、井口泰泉
  • Organizer: 日本動物学会第86回大会
  • Place of Presentation: 朱鷺メッセ（新潟県新潟市）
  • Year and Date: 2015-09-17
• [Presentation] Mechanisms of temperature-dependent determination in the alligator (2015)
  • Author(s): Miyagawa S
  • Organizer: Biosciences Special Seminar
  • Place of Presentation: バーミンガム（イギリス）
  • Int'l Joint Research / Invited
• [Book] Handbook of Hormones, 1st Edition: Comparative Endocrinology for Basic and Clinical Research (2015)
  • Author(s): Iguchi T. (Section editor)
  • Total Pages: 646
  • Publisher: Academic Press
• [Remarks] 宮川信一
  • URL: https://sites.google.com/site/miyashinchi/
• [Remarks] 豊田賢治
  • URL: https://sites.google.com/site/toyotadaphnia/