Molecular physiology of tissue circuit for cold acclimation
| Project/Area Number |
15H04404
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Chromosome dynamics
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| Research Institution | Konan University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Keywords | C. elegans / 低温馴化 / フィードバック制御 / 精子 / 線虫 / 温度応答 / 組織ネットワーク / 温度受容ニューロン |
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| Outline of Final Research Achievements |
To investigate temperature acclimation, we are using cold acclimation in Caenorhabditis elegans. 20°C-cultivated animals did not survival at 2°C. In contrast, 15°C cultivated animals can survive. Previously reported, this cold acclimation is regulated by ASJ temperature sensing-neuron, which releases insulin that is received by intestine and neuron. To identify genes in downstream of insulin-signaling, we performed DNA microarray analysis. We found that expressions of sperm genes were changed in insulin receptor mutants, and sperm mutants showed abnormal cold acclimation. Genetic epistasis analysis suggested that abnormal cold acclimation of sperm mutant was suppressed by the mutations in ASJ. Calcium imaging analysis showed that ASJ neuronal activity in response to temperature was decreased in sperm mutant gsp-4, and rescued by expressing gsp-4 gene in sperm. Thus, we propose a novel feedback between sperm and ASJ temperature sensing-neuron in cold acclimation of C. elgans.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Natural variations of cold tolerance and temperature acclimation in Caenorhabditis elegans2016
Author(s)
Okahata M., Ohta, A., Mizutani, H., Minakuchi, Y., Toyoda, A., Kuhara, A.
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Journal Title
Journal of Comparative Physiology B.
Volume: 186(8)
Issue: 8
Pages: 985-998
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Reconstruction of spatial thermal gradient encoded in thermosensory neuron AFD in Caenorhabditis elegans.2016
Author(s)
Tsukada, Y., Yamao, M., Honda, N., Shimowada, T., Ohnishi, N., Kuhara, A., Ishii, S., Mori, I.
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Journal Title
J Neurosci.
Volume: 36
Issue: 9
Pages: 2571-2581
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Isolation of genes required for cold acclimation speed2016
Author(s)
Shiori Sakai, Tohru Miura, Tomoyo Ujisawa, Yohei Minakuchi, Atsushi Toyoda, Akane Ohta, Atsushi Kuhara
Organizer
C. elegans Neuronal Development, Synaptic Function & Behavior meeting
Place of Presentation
Nagoya university(Nagoya, Aichi Japan)
Year and Date
2016-07-27
Related Report
Int'l Joint Research
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