Experimental demonstration and understanding of host-parasite coevolution

• Project/Area Number: 15H04407
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Evolutionary biology
• Research Institution: Osaka University
• Principal Investigator: Norikazu Ichihashi 大阪大学, 情報科学研究科, 准教授 (20448096)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2017: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2016: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000); Fiscal Year 2015: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
• Keywords: 進化 / 軍拡競争 / RNA / 寄生体 / 共進化 / 宿主 / 実験進化 / 生命の起源 / 生命の初期進化 / RNA複製

Outline of Final Research Achievements

In this project, we aimed to understand possible evolutionary processed between hosts and parasites by constructing a simplified experimental model using self-replicating RNAs. We succeeded the construction and performed a long-term replication experiment of a host RNA with a parasitic RNA. In the replication process, we found that the host RNA evolved a resistance to the parasitic RNA by changing the encoded replicase function, and then the parasite adapt to the changed host RNA by introducing mutations. These results indicate that the host and parasitic RNAs performed an evolutionary arms-race, which can be a useful experimental model to understand coevelutionary process between hosts and parasites.

Research Products

• [Journal Article] Sustainability of a compartmentalized host-parasite replicator system (2018)
  • Author(s): Furubayashi, T., Ichihashi, N.
  • Journal Title: Life Volume: 8 Issue: 1 Pages: 1-10
  • DOI: 10.3390/life8010003
  • Peer Reviewed / Open Access
• [Journal Article] Combinatorial selection for replicable RNA by Qβ replicase while maintaining encoded gene function (2017)
  • Author(s): Yumura, M., Yamamoto, N., Yokoyama, K., Mori, H., Yomo, T., Ichihashi, N.
  • Journal Title: Plos ONE Volume: 12 Issue: 3 Pages: 1-14
  • DOI: 10.1371/journal.pone.0174130
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Host-parasite oscillation dynamics and evolution in a compartmentalized RNA replication system (2016)
  • Author(s): Bansho, Y., Furubayashi, T., Ichihashi, N.*, Yomo, T.*
  • Journal Title: Proc Nat Aca Sci USA Volume: 113 Pages: 4045-4050
  • Peer Reviewed / Open Access
• [Journal Article] A transcription and translation-coupled DNA replication system using rolling-circle replication (2015)
  • Author(s): Sakatani, Y., Ichihashi, N., Kazuta, Y., Yomo, T.
  • Journal Title: Scientific Reports Volume: 5
  • Peer Reviewed / Open Access
• [Journal Article] Development of a reporter peptide that catalytically produces a fluorescent signal through -complementation. (2015)
  • Author(s): Nishiyama, K., Ichihashi, N., Kazuta, Y., Yomo, T.
  • Journal Title: Protein Science Volume: 24 Pages: 599-603
  • Peer Reviewed
• [Journal Article] Activities of 20 aminoacyl-tRNA synthetases expressed in a reconstituted translation system in Escherichia coli (2015)
  • Author(s): Awai, T., Ichihashi, N., Yomo, T.
  • Journal Title: Biochemistry and Biophysics Reports Volume: 3 Pages: 140-143
  • Peer Reviewed / Open Access
• [Journal Article] Periodic Pattern of Genetic and Fitness Diversity during Evolution of an Artificial Cell-Like System (2015)
  • Author(s): Ichihashi, N., Aita, T., Motooka, D., Nakamura, S., Yomo, T.
  • Journal Title: Molecular Biology and Evolution Volume: 32 Pages: 3205-3214
  • Peer Reviewed / Open Access
• [Journal Article] Replication of partial double-stranded RNAs by Qbeta replicase (2015)
  • Author(s): Tomita, K., Ichihashi, N., Yomo, T.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 467 Pages: 293-296
  • Peer Reviewed
• [Journal Article] A design principle for a single-stranded RNA genome that replicates with less double-strand formation (2015)
  • Author(s): Usui, K., Ichihashi, N., Yomo, T.
  • Journal Title: Nucleic Acids Research Volume: 43 Pages: 8033-8043
  • Peer Reviewed / Open Access
• [Journal Article] Maintenance of a translation-coupled RNA replication in dynamic micro-sized compartments under continuous stirring and flow (2015)
  • Author(s): Yoshiyama, T., Motohashi, C., Ichihashi, N., Ichii, T., Yomo, T
  • Journal Title: Chemical Engineering Journal Volume: 283 Pages: 896-902
  • Peer Reviewed
• [Presentation] 実験進化で探る物質から生命への進化可能性 (2018)
  • Author(s): 市橋 伯一
  • Organizer: 生命の起源と進化学会
  • Invited
• [Presentation] Evolution of a self-replicable RNA, the roles of compartment, parasites, and hypercycle (2017)
  • Author(s): Norikazu Ichihashi
  • Organizer: International Conference The Origin of life
  • Int'l Joint Research / Invited
• [Presentation] Host-parasite oscillation dynamics and evolution in a compartmentalized RNA replication system (2017)
  • Author(s): Y. Bansho , T. Furubayashi , N. Ichihashi
  • Organizer: XVIIIth International Conference on the Origin of Life
  • Int'l Joint Research
• [Presentation] Synthesizing an evolvable replication system from bacterial components (2016)
  • Author(s): Ichihashi N
  • Organizer: Synthetic biology workshop
  • Place of Presentation: 奈良先端科学技術大学院大学（奈良県・生駒市）
  • Year and Date: 2016-03-17
  • Int'l Joint Research / Invited
• [Presentation] 人工RNA複製システムの構築と寄生体との共進化 (2015)
  • Author(s): 市橋伯一
  • Organizer: 進化学会年会
  • Place of Presentation: 中央大学（東京都・文京区）
  • Year and Date: 2015-08-20
  • Invited
• [Book] 進化する人工細胞の構築と生命の起源 人工細胞の創製とその応用 第3章2節 (2017)
  • Author(s): 市橋 伯一 他、植田 充美 (監修)
  • Total Pages: 214
  • Publisher: シーエムシー出版
  • ISBN: 9784781312330