Understanding mechanism of formation of radial oxygen loss barrier in maize and teosinte

• Project/Area Number: 15H04434
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Nagoya University
• Principal Investigator: Nakazono Mikio 名古屋大学, 生命農学研究科, 教授 (70282697)
• Co-Investigator(Kenkyū-buntansha): 間野 吉郎 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門, ユニット長 (20355126)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000); Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
• Keywords: トウモロコシ / テオシント / 耐湿性遺伝子 / 酸素漏出バリア / マッピング

Outline of Final Research Achievements

Zea nicaraguensis, which is a wild relative of maize (Z. mays ssp. mays), has higher tolerance of soil waterlogging than maize. We found that Z. nicaraguensis (but not maize) can form a barrier to radial oxygen loss (ROL). To identify a locus controlling ROL barrier formation in Z. nicaraguensis, we used a library of introgression lines (ILs), each containing a chromosome segment from Z. nicaraguensis in the maize inbred line Mi29. By fine-mapping and phenotyping, we discovered that the gene controlling ROL barrier formation was located on about 232 kb region of chromosome 3. We conducted gene expression analyses and then finally found one candidate gene. We also carried out suberin staining of root cross sections. However, we were not able to find any relationship between suberin staining pattern and root ROL barrier formation.

Research Products

• [Int'l Joint Research] 西オーストラリア大学(オーストラリア)
• [Int'l Joint Research] コペンハーゲン大学(デンマーク)
• [Int'l Joint Research] 西オーストラリア大学(オーストラリア)
• [Journal Article] Regulation of root traits for internal aeration and tolerance to soil waterlogging-flooding stress (2018)
  • Author(s): Yamauchi, T., Colmer, T.D., Pedersen, O. and Nakazono, M.
  • Journal Title: Plant Physiology Volume: 176 Issue: 2 Pages: 1118-1130
  • DOI: 10.1104/pp.17.01157
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A major locus involved in the formation of the radial oxygen loss barrier in adventitious roots of teosinte Zea nicaraguensis is located on the short-arm of chromosome 3. (2017)
  • Author(s): Watanabe, K., Takahashi, H., Sato, S., Nishiuchi, S., Omori, F., Malik, A.I., Colmer, T.D., Mano, Y. and Nakazono, M.
  • Journal Title: Plant, Cell & Environment Volume: 40 Issue: 2 Pages: 304-316
  • DOI: 10.1111/pce.12849
  • NAID: 120006305571
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 植物の根への酸素供給システムの制御 (2018)
  • Author(s): 中園幹生
  • Organizer: 第34回資源植物科学シンポジウム/第10回植物ストレス科学研究シンポジウム
  • Invited
• [Presentation] Genetic and physiological analyses of the root traits for internal aeration of crops under flooded soil conditions (2017)
  • Author(s): Mikio Nakazono
  • Organizer: International Symposium on Crop Roots and Rhizosphere Interactions
  • Int'l Joint Research / Invited
• [Presentation] Zea nicaraguensisの酸素漏出バリア形成に関わる遺伝子座の同定 (2017)
  • Author(s): 井出健斗、渡邊宏太郎、髙橋宏和、高橋秀和、大森史恵、間野吉郎、中園幹生
  • Organizer: 日本育種学会第132回講演会
• [Presentation] A locus controlling formation of a barrier to radial oxygen loss is located on chromosome 3 in Zea nicaraguensis (2016)
  • Author(s): Watanabe, K., Takahashi, H., Sato, S., Nishiuchi, S., Omori, F., Malik, A.I., Colmer, T.D., Mano, Y. and Nakazono, M.
  • Organizer: The 12th International Society for Plant Anaerobiosis Conference
  • Place of Presentation: Helsingor, Denmark
  • Year and Date: 2016-09-06
  • Int'l Joint Research