Gene mining of wheat-related wild species for heat tolerant breeding

• Project/Area Number: 15H04437
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Tottori University
• Principal Investigator: Tsujimoto Hisashi 鳥取大学, 乾燥地研究センター, 教授 (50183075)
• Co-Investigator(Renkei-kenkyūsha): MATSUOKA Yoshihiro 福井県立大学, 生物資源学部, 准教授 (80264688) ; ELTAYEB H Amin E 鳥取大学, 乾燥地研究センター, 助教 (00624587) ; OKAMOTO Masanori 宇都宮大学, バオサイエンス教育研究センター, 助教 (50455333)
• Research Collaborator: TAHIR Izzat ; GORAFI Yasir ; ELBASIR Awad
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000); Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: コムギ / 高温障害 / 地球温暖化 / スーダン / 野生種 / 遺伝資源拡大 / DArTマーカー / GWAS / 合成コムギ / 高温ストレス耐性 / 非生物的ストレス耐性 / タルホコムギ / 高温耐性 / 多様性集団 / 高温ストレス

Outline of Final Research Achievements

The applicant produced "Multiple Synthetic Wheat (MSD) Population" which included intraspecific variation of wheat-related wild species, Aegilops tauschii, in a practical wheat genetic background. The MSD population was cultivated in the heat-stressed field in Agricultural Research Corporation (ARC) in Sudan, and together with the breeders, plants showing vigorous growth were selected. We confirmed that the heat tolerance of these genotypes even under controlled high temperature condition in chambers. Meanwhile, 400 individuals were randomly selected from the MSD population, cultivated in three sites of ARC, and the agronomical traits were measured. In addition, these lines were genotyped with a large numbers of markers. The high-temperature resistant wheat breeding platform constructed in this study is useful for selection and marker assist selection of high temperature tolerant cultivars and for elucidation of high temperature tolerance mechanism.

Research Products

• [Int'l Joint Research] 農業研究機構(スーダン)
• [Int'l Joint Research] 農業研究機構(スーダン)
• [Int'l Joint Research] 農業研究機構(スーダン)
• [Journal Article] Wheat multiple synthetic derivatives: a new source for heat stress tolerance adaptive traits (2017)
  • Author(s): Elbashir Awad Ahmed Elawad、Gorafi Yasir Serag Alnor、Tahir Izzat Sidahmed Ali、Kim June-Sik、Tsujimoto Hisashi
  • Journal Title: Breeding Science Volume: 67 Issue: 3 Pages: 248-256
  • DOI: 10.1270/jsbbs.16204
  • NAID: 130005765959
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genetic variation in heat tolerance-related traits in a population of wheat multiple synthetic derivatives (2017)
  • Author(s): Elbashir Awad A. E.、Gorafi Yasir S. A.、Tahir Izzat S. A.、Elhashimi Ashraf. M. A.、Abdalla Modather G. A.、Tsujimoto Hisashi
  • Journal Title: Breeding Science Volume: 67 Issue: 5 Pages: 483-492
  • DOI: 10.1270/jsbbs.17048
  • NAID: 130006318283
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Wheat multiple synthetic derivatives: a new source for heat stress tolerance adaptive traits (2017)
  • Author(s): Elbashir AAE, Gorafi YSA, Tahir ISA, Kim J-S, and Tsujimoto H
  • Journal Title: Breeding Science Volume: 67
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Wild gene mining for sustainable wheat production under changing climate (2018)
  • Author(s): Tsujimoto H
  • Organizer: 11th International Symposium Exploring the Global Sustainability
  • Int'l Joint Research / Invited
• [Presentation] MSD population: New source of heat tolerance of wheat in high-temperature environments. (2017)
  • Author(s): Elbashir, A. A. E. , Gorafi, Y. S. A., Tahir, I. S. A., Elhashimi, A., Abdeldaim, M. and Tsujimoto, H.
  • Organizer: 日本育種学会第131回講演会
  • Place of Presentation: 名古屋
  • Year and Date: 2017-03-29
• [Presentation] 高温ストレス下のパンコムギにおける種子貯蔵タ ンパク質の発現解析と小麦粉品質 (2017)
  • Author(s): 田中 裕之, Yasir S. A. Gora, 藤田 基寛, 佐々木 悠, Izzat S. A. Tahir, 辻本 壽
  • Organizer: 日本育種学会第132回講演会
• [Presentation] この実験材料がアフリカのコムギ生産に貢献できるの？～その不安と喜び～ (2017)
  • Author(s): 辻本 壽
  • Organizer: 日本育種学会第59回シンポジウム
• [Presentation] Genome wide association study of grain yield and related traits in MSD population under heat stress environments. (2016)
  • Author(s): Elbashir, A. A. E., Gorafi, Y. S. A., Kim, J.-K, Tahir, I. S. A, Elhashimi, A., Abdeldaim, M. and Tsujimoto, H.
  • Organizer: 日本育種学会第130回講演会
  • Place of Presentation: 鳥取
  • Year and Date: 2016-09-24
• [Presentation] Genome wide association study of grain yield and related traits in bread wheat of MSD population under heat stress environments. (2016)
  • Author(s): Elbashir, A. A. E., Gorafi, Y. S. A., Kim, J.-K., Tahir, I. S. A., Elhashimi, A., Abdeldaim, M. and Tsujimoto, H.
  • Organizer: The 12th International Dryland Development Conference
  • Place of Presentation: Alexandria, Egypt
  • Year and Date: 2016-08-21
  • Int'l Joint Research
• [Presentation] Genome wide association study of wheat heat tolerance in MSD population (2016)
  • Author(s): Elbashir, A, Gorafi Y, Kim, J, Tsujimoto H
  • Organizer: 日本育種学会第129回講演会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-21