Analysis on the phosphatase regulated ethylene biosynthesis by phosphorylated ACC synthase.

• Project/Area Number: 15H04450
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Horticultural science
• Research Institution: Nagoya University
• Principal Investigator: Mori Hitoshi 名古屋大学, 生命農学研究科, 教授 (20220014)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000); Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: エチレン生合成 / ACC合成酵素 / リン酸化 / 脱リン酸化 / protein phosphatase / ホスファーターゼ / ホスファターゼ / エチレン / フォスファターゼ

Outline of Final Research Achievements

ACC synthase, ACS, which is rate limiting key enzyme of plant hormone ethylene biosynthesis is phosphorylated by protein kinase CDPK and MAPK respectively. Phosphorylated ACS is stable and dephosphorylated one is unstable in cell. I identified B”type subunit of PP2A type protein phosphatase in tomato as candidate that recognizes ACS as target.

Research Products

• [Journal Article] Genome of pitcher plant Cephalotus reveals genetic changes associated with carnivory (2017)
  • Author(s): Fukushima, K. et al.
  • Journal Title: Nat. Ecol. Evol. Volume: 1 Issue: 3 Pages: 0059-0059
  • DOI: 10.1038/s41559-016-0059
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An NADPH oxidase RBOH functions in rice roots during lysigenous aerenchyma formation under oxygen-deficient conditions. (2017)
  • Author(s): Yamauchi, T., Yoshioka, M., Fukazawa, A., Mori, H., Nishizawa, N.K., Tsutsumi, N., Yoshioka, H. and Nakazono, M.
  • Journal Title: The Plant Cell Volume: 印刷中 Issue: 4 Pages: 775-790
  • DOI: 10.1105/tpc.16.00976
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The AMOR arabinogalactan sugar chain induces pollen tube competency to respond to ovular guidance (2016)
  • Author(s): Mizukami A., Inatsugi R., Jiao J., Kotake T., Kuwata K., Ootani K, Okuda S., Sankaranarayanan S., Sato Y., Maruyama D., Iwai H., Garenaux E., Sato C., Kitajima K., Tsumuraya Y., Mori H., Yamaguchi J., Itami K., Sasaki N. and Higashiyama T.
  • Journal Title: Curr. Biol. Volume: 26 Issue: 8 Pages: 1091-1097
  • DOI: 10.1016/j.cub.2016.02.040
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of a xylem sap germin-like protein and its expression under short-day and non-freezing low-temperature conditions in poplar root. (2016)
  • Author(s): Aohara, T., Mizuno, H., Kiyomichi, D., Abe, Y., Matsuki, K., Sagawa, K., Mori, H., Iwai, H., Furukawa, J. and Satoh, S.
  • Journal Title: Plant Biotech. Volume: 33 Pages: 123-127
  • NAID: 130005164807
  • Peer Reviewed
• [Journal Article] Loss of function at RAE2, a novel EPFL, is required for awnlessness in cultivated Asian rice. (2016)
  • Author(s): Bessho-Uehara K, Wang DR, Furuta T, Minami A, Nagai K, Gamuyao R, Asano A, Angeles-Shim RB, Shimizu Y, Ayano M, Komeda N, Doi K, Miura K, Toda Y, Kinoshita T, Okuda S, Higashiyama T, Nomoto M, Tada Y, Shinohara H, Matsubayashi Y, Greenberg A, Wu J, Yasui H, Yoshimura A, Mori H, McCouch SR, Ashikari M.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 113 Issue: 32 Pages: 8969-8974
  • DOI: 10.1073/pnas.1604849113
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ethylene Biosynthesis Is Promoted by Very-Long-Chain Fatty Acids during Lysigenous Aerenchyma Formation in Rice Roots (2015)
  • Author(s): Yamauchi T, Shiono K, Nagano M, Fukazawa A, Ando M, Takamure I, Mori H, Nishizawa NK, Kawai-Yamada M, Tsutsumi N, Kato K, Nakazono M
  • Journal Title: Plant Physiology Volume: 169 Issue: 1 Pages: 180-193
  • DOI: 10.1104/pp.15.00106
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rare allele of a previously unidentified histone H4 acetyltransferase enhances grain weight, yield, and plant biomass in rice. (2015)
  • Author(s): Song XJ, Kuroha T, Ayano M, Furuta T, Nagai K, Komeda N, Segami S, Miura K, Ogawa D, Kamura T, Suzuki T, Higashiyama T, Yamasaki M, Mori H, Inukai Y, Wu J, Kitano H, Sakakibara H, Jacobsen SE, Ashikari M.
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 112 Issue: 1 Pages: 76-81
  • DOI: 10.1073/pnas.1421127112
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] ラナンキュラス塊根の吸水発芽時に発現するα-galactosidaseタンパク質の発現解析 (2018)
  • Author(s): 森仁志, 村上恭子
  • Organizer: 園芸学会 平成30年度春季大会