Studies on the function of the fungal effector NIS1 for suppression of plant immunity

• Project/Area Number: 15H04457
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Kyoto University
• Principal Investigator: Takano Yoshitaka 京都大学, 農学研究科, 教授 (80293918)
• Co-Investigator(Renkei-kenkyūsha): NISHIUCHI TAKUMI 金沢大学, 学際科学実験センター, 准教授 (20334790)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000); Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2015: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
• Keywords: 植物病原糸状菌 / エフェクター / 抵抗性 / PAMPs / タンパク質リン酸化酵素 / PAMP / パターン認識受容体 / 炭疽病菌 / 活性酸素 / イネいもち病菌 / 植物病理学 / 植物免疫 / BAK1 / BIK1 / 糸状菌

Outline of Final Research Achievements

Plant pathogenic fungi establish the infection of their host plants by using a set of secreted proteins called effectors. This study focused on the effector NIS1 identified in a cucumber anthracnose fungus Colletotrichum orbiculare. Plants activate defense responses via the recognition of Pathogen-Associated Molecular Patterns (PAMPs). The study revealed that NIS1, widely conserved in plant pathogenic fungi, targets protein kinases critical for the PAMP-triggered immunity and plays important roles for infection strategies of plant pathogenic fungi including Colletotrichum species.

Research Products

• [Journal Article] Glutathione transferase U13 functions in pathogen-triggered glucosinolate metabolism (2018)
  • Author(s): Pislewska-Bednarek M, Nakano RT, Hiruma K, Pastorczyk M, Sanchez-Vallet A, Singkaravanit-Ogawa S, Ciesiolka D, Takano Y, Molina A, Schulze-Lefert P, and Bednarek P.
  • Journal Title: Plant Physiology Volume: 176 Issue: 1 Pages: 538-551
  • DOI: 10.1104/pp.17.01455
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Inappropriate expression of an NLP effector in Colletotrichum orbiculare impairs infection on Cucurbitaceae cultivars via plant recognition of the C-terminal region. (2018)
  • Author(s): Nur Sabrina Ahmad Azmi, Suthitar Singkaravanit-Ogawa S, Kyoko Ikeda, Saeko Kitakura, Yoshihiro Inoue, Yoshihiro Narusaka, Ken Shirasu, Masanori Kaido, Kazuyuki Mise, and Yoshitaka Takano
  • Journal Title: Mol. Plant-Microbe Interact. Volume: 31 Issue: 1 Pages: 101-111
  • DOI: 10.1094/mpmi-04-17-0085-fi
  • Peer Reviewed
• [Journal Article] Draft genome assembly of Colletotrichum chlorophyti, a pathogen of herbaceous plants (2017)
  • Author(s): Gan, P., Narusaka, M., Tsushima, A., Narusaka, Y., Takano, Y. and Shirasu, K.
  • Journal Title: Genome Announcement Volume: 5 Issue: 10
  • DOI: 10.1128/genomea.01733-16
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Dysfunction of Arabidopsis MACPF domain protein activates programmed cell death via tryptophan metabolism in MAMP-triggered immunity (2017)
  • Author(s): Fukunaga S, Sogame M, Hata M, Singkaravanit-Ogawa S, Pislewska-Bednarek M, Onozawa-Komori M, Nishiuchi T, Hiruma K, Saitoh H, Terauchi R, Kitakura S, Inoue Y, Bednarek P, Schulze-Lefert P, and Takano Y.
  • Journal Title: Plant Journal Volume: 89 Issue: 2 Pages: 381-393
  • DOI: 10.1111/tpj.13391
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Regulation of sugar transporter activity for antibacterial defense in Arabidopsis (2016)
  • Author(s): Yamada K, Saijo Y, Nakagami H, Takano Y
  • Journal Title: Science Volume: 354(6318) Issue: 6318 Pages: 1427-1430
  • DOI: 10.1126/science.aah5692
  • NAID: 120005895801
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Genus-wide comparative genome analyses of Colletotrichum species reveal specific gene family losses and gains during adaptation to specific infection lifestyles. (2016)
  • Author(s): Gan, P., Narusaka, M., Kumakura, N., Tsushima, A., Takano, Y., Narusaka, Y., Shirasu, K.
  • Journal Title: Genome Biology and Evolution. Volume: 8 Issue: 5 Pages: 1467-1481
  • DOI: 10.1093/gbe/evw089
  • NAID: 120006822486
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Focal effector accumulation in a biotrophic interface at the primary invasion sites of Colletotrichum orbiculare in multiple susceptible plants. (2016)
  • Author(s): Hiroki Irieda, Suthitar Ogawa and Yoshitaka Takano.
  • Journal Title: Plant Signaling & Behavior Volume: 11 Issue: 2 Pages: e1137407-e1137407
  • DOI: 10.1080/15592324.2015.1137407
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification and characterization of virulence- related effectors in the cucumber anthracnose fungus Colletotrichum orbiculare (2016)
  • Author(s): Hiroki Irieda and Yoshitaka Takano.
  • Journal Title: Physiological and Molecular Plant Pathology Volume: 94 Pages: 87-92
  • DOI: 10.1016/j.pmpp.2016.01.006
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1 (2016)
  • Author(s): Narusaka M, Toyoda K, Shiraishi T, Iuchi S, Takano Y, Shirasu K, Narusaka Y.
  • Journal Title: Scientifc Reports Volume: 6 Issue: 1 Pages: 18702-18702
  • DOI: 10.1038/srep18702
  • NAID: 120005819504
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Oil body-mediated defense against fungi : From tissues to ecology (2015)
  • Author(s): Shimada TL, Takano Y, Hara-Nishimura I.
  • Journal Title: Plant Signal Behav Volume: 10 Issue: 2 Pages: 1559-1559
  • DOI: 10.4161/15592324.2014.989036
  • NAID: 120005646470
  • Peer Reviewed
• [Presentation] 比較ゲノム・トランスクリプトーム解析によるウリ類炭疽病菌の強病原性関連因子の探索 (2018)
  • Author(s): 井上 喜博・Pamela Gan・鳴坂 義弘・白須 賢・高野 義孝
  • Organizer: 平成30年度日本植物病理学会
• [Presentation] ウリ類炭疽病菌の宿主特異性に関する研究：アルファルファ炭疽病菌との比較解析 (2018)
  • Author(s): 小川泰生・井上喜博・Pamela Gan・海道真典・三瀬和之・鳴坂 義弘・白須 賢・高野義孝
  • Organizer: 平成30年度日本植物病理学会
• [Presentation] ウリ類炭疽病菌は転写制御因子SGE1依存的にメロン根部へ感染し植物体の萎凋症状を引き起こす (2018)
  • Author(s): 田中冬樹・Suthitar Singkaravanit-Ogawa・海道真典・三瀬和之・高野義孝
  • Organizer: 平成30年度日本植物病理学会
• [Presentation] 植物と炭疽病菌の攻防戦 (2018)
  • Author(s): 高野義孝
  • Organizer: 第10回植物ストレス科学研究シンポジウム
  • Invited
• [Presentation] ホスファチジルイノシトールリン酸を介した炭疽病菌感染機構の解明 (2017)
  • Author(s): 島田 貴士、高野 義孝、別役 重之、中野 明彦、上田 貴志
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学郡元キャンパス
  • Year and Date: 2017-03-16
• [Presentation] 炭疽病菌と植物の相互作用：エフェクター研究を中心に (2017)
  • Author(s): 高野義孝
  • Organizer: 第17回糸状菌分子生物学コンファレンス
  • Invited
• [Presentation] Involvement of tryptophan-derived secondary metabolism in post-invasive resistance of Arabidopsis thaliana against fungal pathogens (2017)
  • Author(s): Kosaka A, Bednarek P, Ishikawa A, Kaido M, Mise K, and Takano Y.
  • Organizer: 平成29年度日本植物病理学会関西部会
• [Presentation] ウリ類炭疽病菌RSCO-09-1-2株の強病原性に関する分子機構研究 (2017)
  • Author(s): 井上 喜博・Pamela Gan・鳴坂 義弘・白須 賢・高野 義孝
  • Organizer: 平成29年度日本植物病理学会大会
• [Presentation] Inappropriate expression of NLP effector impairs Colletotrichum infection on cucurbits via recognition of its C-terminal region (2017)
  • Author(s): Singkaravanit-Ogawa S, Nur Sabrina AA, Ikeda K, Tanaka S, Inoue Y, Kaido M, Mise K, Narusaka Y, Shirasu K, and Takano Y.
  • Organizer: 平成29年度日本植物病理学会大会
• [Presentation] Inappropriate expression of the NLP effector impairs the infection of Colletotrichum orbiculare on cucumber (2016)
  • Author(s): Nur Sabrina Ahmad Azmi, Suthitar Singkaravanit-Ogawa, Kyoko Ikeda, Masanori Kaido, Kazuyuki Mise, and Yoshitaka Takano
  • Organizer: 平成28年度日本植物病理学会関西部会
  • Place of Presentation: 静岡県コンベンションセンター
  • Year and Date: 2016-09-29
• [Presentation] ウリ類炭疽病菌の強病原性株RSCO-09-1-2の強病原性化因子の探索 (2016)
  • Author(s): 井上喜博、Pamela Gan、鳴坂義弘、白須 賢、高野義孝
  • Organizer: 平成28年度日本植物病理学会関西部会
  • Place of Presentation: 静岡県コンベンションセンター
  • Year and Date: 2016-09-29
• [Presentation] Comparative genomics of Colletotrichum fungi reveals lifestyle-adapted fungal gene gain/loss andn potential virulence-associated genes (2016)
  • Author(s): Pamela Gan, Mari Narusaka, Naoyoshi Kumakura, Ayako Tsushima, Ryoko Hiroyama, Yoshitaka Takano, Yoshihiro Narusaka, Ken Shirasu
  • Organizer: European Fungal Genomics Conference 13
  • Place of Presentation: PARIS-LA VILLETTE
  • Year and Date: 2016-04-03
  • Int'l Joint Research
• [Presentation] 糸状菌エフェクターNIS1は高等植物のPRR複合体における複数の因子を攻撃する (2016)
  • Author(s): 入枝泰樹、森正之、山田晃嗣、押川友、大木進野、齋藤宏昌、寺内良平、高野義孝
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2016-03-21
• [Presentation] ウリ類炭疽病菌の強病原性株RSCO-09-1-2に関する研究 (2016)
  • Author(s): 井上喜博、Pamela Gan、鳴坂義弘、白須 賢、高野義孝
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2016-03-21
• [Presentation] ウリ類炭疽病菌の付着器において発現するエフェクター候補群ECAPの機能解析 (2016)
  • Author(s): 奥田竜太、石塚隼也、Suthitar Singkaravanit-Ogawa、Pamela Gan、山田晃嗣、鳴坂義弘、白須 賢、高野義孝
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2016-03-21
• [Presentation] 比較ゲノム解析を用いたColletotrichum属菌における病原性エフェクターの探索 (2016)
  • Author(s): 熊倉直祐、Pamela Gan、津島綾子、浅井秀太、門田康弘、鳴坂真理、鳴坂義弘、高野義孝、白須賢
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2016-03-21
• [Presentation] コアエフェクター候補遺伝子CCE1はColletotrichum属菌に保存され、細胞死を誘導する (2016)
  • Author(s): 津島 綾子、鳴坂 真理、Pamela Gan、熊倉 直祐、浅井 秀太、門田 康弘、高野 義孝、鳴坂 義弘、白須 賢
  • Organizer: 平成28年度日本植物病理学会大会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2016-03-21
• [Presentation] Effectors in Colletotrichum orbiculare (2015)
  • Author(s): Yoshitaka Takano
  • Organizer: 11th US-Japan Scientific Seminar
  • Place of Presentation: Kagawa international conference hall
  • Year and Date: 2015-10-25
  • Int'l Joint Research / Invited
• [Presentation] 炭疽病菌の基本的親和性とエフェクター (2015)
  • Author(s): 高野義孝
  • Organizer: 平成27年度日本植物病理学会関西部会若手の会
  • Place of Presentation: 徳島県あわぎんホール
  • Year and Date: 2015-09-28
  • Invited