Mechanism of Biogenesis of Membrane Microdomain Containing Polyunsaturated Fatty Acids in Bacteria and Its Physiological Functions
| Project/Area Number |
15H04474
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
川本 純 京都大学, 化学研究所, 助教 (90511238)
小川 拓哉 京都大学, 化学研究所, 助教 (40756318)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Keywords | 生体膜 / 高度不飽和脂肪酸 / エイコサペンタエン酸 / リン脂質 / アシル化タンパク質 / マイクロドメイン / 細菌 |
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| Outline of Final Research Achievements |
We synthesized an ω-ethynyl analog of eicosapentaenoic acid (EPA) as a tool for analyzing the in vivo behavior and function of EPA. The analog, named eEPA, has an ω-ethynyl group tag in place of the ω-methyl group of EPA. By using eEPA, we obtained results suggesting that the subcellular distribution of EPA in an EPA-producing bacterium is different from that of palmitoleic acid and that EPA is required for normal assembly of a cell division-related protein, FtsZ. We also found the occurrence of EPA-modified proteins.
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| Academic Significance and Societal Importance of the Research Achievements |
ω3系の高度不飽和脂肪酸 (PUFA) はヒトの健康維持に重要な機能性脂肪酸として注目されている。本研究はω3系PUFAの一種であるEPAを生産する細菌を用いて、EPAの細胞内動態や生理機能発現機構の一端を明らかにした点で学術的意義がある。本研究で開発したEPAアナログ分子は、ヒトを含む多様な生物におけるEPAの動態や機能発現機構を解析するためのプローブ分子として有用と考えられる。
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Report
(4 results)
Research Products
(47 results)
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[Presentation] Kawamoto J, Kurihara T2016
Author(s)
Physiological function of polyunsaturated fatty acids in microbial cold and high-pressure adaptation
Organizer
5th International Workshop on Deep-Sea Microbiology
Place of Presentation
京都大学（京都市）
Year and Date
2016-09-10
Related Report
Int'l Joint Research / Invited
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