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The hidden world of jumbo phages

Research Project

Project/Area Number 15H04477
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionHiroshima University

Principal Investigator

Yamada Takashi  広島大学, 先端物質科学研究科, 名誉教授 (40230461)

Co-Investigator(Kenkyū-buntansha) 川崎 健  広島大学, 先端物質科学研究科, 助教 (00510299)
藤江 誠  広島大学, 先端物質科学研究科, 准教授 (20274110)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywords青枯病 / Ralstonia solanacearum / ジャンボファージ / ゲノム研究 / バイオコントロール / ゲノム解読 / Ralston solanacearum
Outline of Final Research Achievements

Ralstonia jumbo phages RSL2-RSL4 have been characterized for their genomic, proteomic, phylogenetic, and virological features. Phylogenetically, RSL2, RSL3, and RSL4 are closely related to Pseudomonas jumbo phage phiKZ, whereas RSL5 is similar to RSL1 and related to Bacillus phage G. These 4 phages share a common long-lasting infection cycle with host Ralstonia cells, which gives a huge advantage for them to be utilized as biocontrol agents with sustainable effects on plant diseases (bacterial wilt).

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (28 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (2 results) Journal Article (14 results) (of which Int'l Joint Research: 13 results,  Peer Reviewed: 11 results,  Open Access: 8 results,  Acknowledgement Compliant: 6 results) Presentation (9 results) (of which Int'l Joint Research: 2 results) Remarks (3 results)

  • [Int'l Joint Research] UMI3265 UJF-EMBL-CNRS(France)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] NSTDA, BIOTEC(タイ)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Chitin syn thesis by Chlorella Cells infected by Chloroviruses: Enhancement by adopting a slow-growing virus and treatment with aphidicolin2018

    • Author(s)
      Numfon Rakkhumkaew, Takeru Kawasaki, Makoto Fujie, and Takashi Yamada
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 125 Issue: 3 Pages: 311-315

    • DOI

      10.1016/j.jbiosc.2017.10.002

    • NAID

      40021507541

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Xanthomonas citri Jumbo Phage XacN1 Exhibits a Wide Host Range and High Complement of tRNA Geness2018

    • Author(s)
      Genki Yoshikawa, Ahmed Askora, Romain Blanc-Mathieu, Takeru Kawasaki, Yanze, Li, Miyako Nakano, Hiroyuki Ogata, and Takashi Yamada
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 4486-4486

    • DOI

      10.1038/s41598-018-22239-3

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Replications of Two Closely Related Groups of Jumbo Phages Show Different Level of Dependence on Host-encoded RNA Polymerase2017

    • Author(s)
      Matsui T, Yoshikawa G, Mihara T, Chatchawankanphanich O, Kawasaki T, Nakano M, Fujie M, Ogata H, Yamada T.
    • Journal Title

      Front Microbiol.

      Volume: 8 Pages: 1010-1010

    • DOI

      10.3389/fmicb.2017.01010

    • NAID

      120006331975

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dynamic integration and excision of filamentous phage XacF1 in Xanthomonas citri pv. citri, the causative agent of citrus canker disease.2017

    • Author(s)
      Ahmad AA, Kawabe M, Askora A, Kawasaki T, Fujie M, Yamada T.
    • Journal Title

      FEBS Open Bio.

      Volume: 7(11) Issue: 11 Pages: 1715-1721

    • DOI

      10.1002/2211-5463.12312

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Lysogenic Conversion of the Phytopathogen Ralstonia solanacearum by the P2virus RSY1.2017

    • Author(s)
      Askora A, Kawasaki T, Fujie M, Yamada T.
    • Journal Title

      Front Microbiol.

      Volume: 8 Pages: 2212-2212

    • DOI

      10.3389/fmicb.2017.02212

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Isolation and characterization of bacteriophages infecting Ralstonia solanacearum in Thailand2017

    • Author(s)
      A. Bhunchoth, N. Phironrit, C. Leksomboon, T. Kawasaki, T. Yamada and O. Chatchawankanphanich
    • Journal Title

      Acta Horticulturae

      Volume: in press

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] The involvement of the PilQ secretin of type IV pili in phage infection in Ralstonia solanacearum2016

    • Author(s)
      Narulita, E., H. S. Addy, T. Kawasaki, M. Fujie and T. Yamada.
    • Journal Title

      Biochem. Biophys. Res. Comm.

      Volume: 469 Issue: 4 Pages: 868-872

    • DOI

      10.1016/j.bbrc.2015.12.071

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A Ralstonia solanacearum phage phiRP15 is closely related to Viunalikeviruses and encodes 19 tRNA-related sequences2016

    • Author(s)
      Tomoko Mihara, Mohamed A. Nasr-Eldin, Orawan Chatchawankanphanich, Anjana Bhunchoth, Namthip Phironrit, Takeru Kawasaki, Miyako Nakano, Makoto Fujie, Hiroyuki Ogata, Takashi Yamada
    • Journal Title

      Virology Reports

      Volume: 6 Pages: 61-73

    • DOI

      10.1016/j.virep.2016.07.001

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Technical Report: To create one (1) new genus, Rslunavirus, including one (1) new species, within the Myoviridae.2016

    • Author(s)
      E. M. Adriaenssens, J. H. Kuhn, A. M. Kropinski, Takashi Yamada
    • Journal Title

      ICTV Taxoprop

      Volume: 2016. 043a-dB

    • DOI

      10.13140/RG.2.2.16922.24000

    • Related Report
      2016 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Journal Article] Technical Report: To create one (1) new genus, Rsl2virus, including one (2) new species, within the Myoviridae.2016

    • Author(s)
      E. M. Adriaenssens, J. H. Kuhn, A. M. Kropinski, Takashi Yamada
    • Journal Title

      ICTV Taxoprop

      Volume: 2016. 042a-dB

    • DOI

      10.13140/RG.2.2.10211.35364

    • Related Report
      2016 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Journal Article] バイオよもやま話:いまどき?いまこそ!プラークアッセイ -新奇ジャンボファージ取得のためのプラークアッセイのすゝめ-2016

    • Author(s)
      川崎 健、山田 隆
    • Journal Title

      生物工学会誌

      Volume: 94 Pages: 492-495

    • Related Report
      2016 Annual Research Report
    • Acknowledgement Compliant
  • [Journal Article] Genomic diversityoflarge-plaque-formingpodovirusesinfectingthe phytopathogen Ralstoniasolanacearum2016

    • Author(s)
      Takeru Kawasaki 他10名
    • Journal Title

      Virology

      Volume: 492 Pages: 73-81

    • DOI

      10.1016/j.virol.2016.02.011

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Two Asian jumbo phages, phiRSL2 and phiRSF1, infect Ralstonia solanacearum and show common features of phiKZ-related phages2016

    • Author(s)
      Anjana Bhunchoth 他12名
    • Journal Title

      Virology

      Volume: 494 Pages: 56-66

    • DOI

      10.1016/j.virol.2016.03.028

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Isolation of Ralstonia solanacearum-infecting bacteriophages from tomato fields in Chiang Mai, Thailand, and their experimental use as biocontrol agents2015

    • Author(s)
      Anjana Bhunchoth 他8名
    • Journal Title

      J. Appl. Microbiol.

      Volume: 118 Issue: 4 Pages: 1023-1033

    • DOI

      10.1111/jam.12763

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Modified Method to Isolate Jumbo Bacteriophages for Use in Biocontrol of a Wide-range of Pathogenic Bacteria2018

    • Author(s)
      Alaaeldin M. Saad、Ahmed M. Soliman, Takeru Kawasaki, Makot Fujie, Hirofumi Nariya, Tadashi Shimamoto, Takashi Yamada,
    • Organizer
      ASM Microbe 2018 (Atlanta, GA, USA)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Smart utilization of jumbo phages for biological control of zoonotic diseases2017

    • Author(s)
      Alaaeldin M. Saad、Ahmed M. Soliman, Hirofumi Nariya, Tadashi Shimamoto, Takashi Yamada, Takeru Kawasaki
    • Organizer
      第69回日本生物工学会大会(東京)
    • Related Report
      2017 Annual Research Report
  • [Presentation] カンキツかいよう病菌に感染する大型ファージXacN1のゲノム解析による特徴づけ2016

    • Author(s)
      吉川元貴、R. Blanc-Mathieu,緒方博之、山田 隆
    • Organizer
      第31回日本微生物生態学会大会
    • Place of Presentation
      横須賀市文化会館
    • Year and Date
      2016-10-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] 青枯病菌に感染するViunalikevirus, RP15の単離と解析2016

    • Author(s)
      川崎 健、三原知子、緒方博之、藤江 誠、山田 隆
    • Organizer
      平成28年度日本生物工学会大会
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 宿主病原性に影響を与える溶原性繊維状ファージRSSの溶原/誘発機構の解析2015

    • Author(s)
      川崎 健、藤江 誠,山田 隆
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-10-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] 青枯病菌に感染するジャンボファージの解析と青枯病防除への利用2015

    • Author(s)
      松井 健、山田 隆、川崎 健、藤江 誠
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-10-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] ファージを利用した青枯病防除技術の開発:ジャンボファージ及びT7型ファージの有効性2015

    • Author(s)
      安田太輝、杉村祐哉、山田 隆、藤江 誠、川崎 健
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-10-28
    • Related Report
      2015 Annual Research Report
  • [Presentation] Importance of PilQ on the infection by several types of bacteriophages2015

    • Author(s)
      E. Narulita, T. Kawasaki, M. Fujie, and T. Yamada
    • Organizer
      第67回日本生物工学会大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2015-10-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] Genomic diversity of large-plaque-forming podoviruses infecting the phytopathogen Ralstonia solanacearum2015

    • Author(s)
      E. Narulita, T. Kawasaki, M. Fujie, and T. Yamada
    • Organizer
      International Meeting: Molecular Genetics of Bacteria and Phages
    • Place of Presentation
      University of Wisconsin-Madison, WI, USA
    • Year and Date
      2015-08-04
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 先端研生命分子情報学研究室(山田研)一講ホームページへようこそ!

    • URL

      http://home.hiroshima-u.ac.jp/mbiotech/ichikou/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 広島大学大学院先端物質科学研究科生命分子情報学研究室

    • URL

      http://home.hiroshima-u.ac.jp/mbiotech/ichikou/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 生命分子情報学研究室 広島大学先端物質科学研究科

    • URL

      http://home.hiroshima-u.ac.jp/mbiotech/ichikou/index.html

    • Related Report
      2015 Annual Research Report

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Published: 2015-04-16   Modified: 2022-09-27  

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