Functional analysis of triterpenoid biosynthetic genes in plant by using genome editing
| Project/Area Number |
15H04485
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
福島 エリオデット 大阪大学, 工学研究科, 助教 (40724448)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SEKI Hikaru 大阪大学, 大学院工学研究科, 准教授 (30392004)
|
|---|
| Research Collaborator |
YASUMOTO Shuhei
SUZUKI Hayato
TAMURA Keita
ROBERTLEE Jekson
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
|
|---|
| Keywords | ゲノム編集 / 植物組織培養 / テルペノイド / 代謝制御 / 植物特化代謝物 / 合成生物学 / P450 / トリテルペノイド / 組織培養 / バイオテクノロジー / 植物 / 代謝工学 / トマト / シロイヌナズナ / カンゾウ / 酵母 |
|---|
| Outline of Final Research Achievements |
Plants produce a wide variety of secondary (specialized) metabolites to survive under biotic and abiotic stress. Among them, terpenoids are those of the most structurally varied class of plant specialized metabolites and perform multiple important biological roles. Cytochrome P450s (CYPs) have a central role to enhance the diversity of the triterpenoids, C-30 terpenoids. In this study, we analyzed the function of various CYPs in triterpenoid biosynthesis using yeast heterologous expression system. Furthermore, we created gene knockout plant/plant tissues to confirm in planta function of those CYPs including Arabidoisis, tomato and licorice.
|
Report
(4 results)
Research Products
(52 results)
-
-
-
-
-
-
-
[Journal Article] Isolation and characterization of the soybean Sg-3 gene that is involved in genetic variation in sugar chain composition at the C-3 position in soyasaponin2018
Author(s)
Yano R, Takagi K, Tochigi S, Fujisawa Y, Nomura Y, Tsuchinaga H, Takahashi Y, Takada Y, Kaga A, Anai T, Tsukamoto C, Seki H, Muranaka T, Ishimoto M
-
Journal Title
Plant Cell Physiol.
Volume: 59
Issue: 4
Pages: 797-810
DOI
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] CYP716A179 functions as a triterpene C-28 oxidase in tissue-cultured stolons of Glycyrrhiza uralensis.2017
Author(s)
Tamura, K., Seki, H., Suzuki, H., Kojoma, M., Saito, K. and Muranaka, T.: CYP716A179 functions as a triterpene C-28 oxidase in tissue-cultured stolons of Glycyrrhiza uralensis.
-
Journal Title
Plant Cell Rep.
Volume: 36
Issue: 3
Pages: 437-445
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
-
-
[Journal Article] Platform for “Chemical Metabolic Switching” to Increase Sesquiterpene Content in Plants2017
Author(s)
K. Kobayashi, K. Kobayashi, H. Yamaguchi, Y. M. Inoue, K. Takagi, K. Fushihara, H. Seki, M. Suzuki, N. Nagata, T. Muranaka
-
Journal Title
Plant Biotechnology
Volume: 34
Issue: 1
Pages: 65-69
DOI
NAID
ISSN
1342-4580, 1347-6114
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
