Study on structure and function of the processing enzyme for pre-tRNA and its application

• Project/Area Number: 15H04487
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Kyushu University
• Principal Investigator: Kimura Makoto 九州大学, 農学研究院, 学術特任教員 (10204992)
• Co-Investigator(Kenkyū-buntansha): 中島 崇 九州大学, 農学研究院, 助教 (20380553) ; 角田 佳充 九州大学, (連合)農学研究科(研究院), 教授 (00314360)
• Co-Investigator(Renkei-kenkyūsha): MAYANAGI Kota 九州大学, 生体防御医学研究所, 助教 (50418571)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: リボヌクレアーゼP / アーキア / tRNAプロセシング酵素 / 超好熱性アーキア / リボヌクレアーゼ P / 透過型電子顕微鏡 / リボヌクレアーゼＰ / 前駆体tRNA / リボ核タンパク質 / RNA結合タンパク質 / 前駆体tRNAのプロセシング / リボ核タンパク質複合体酵素 / 生体触媒の分子進化

Outline of Final Research Achievements

Ribonuclease P (RNase P) is a ribonucleoprotein that cleaves off 5´ leader sequences from tRNA precursors in all living cells and consists of a catalytic RNA and protein cofactors. In this study, I performed the structure and functional study of RNase P from the hyperthermophilic archaeon Pyrococcus horioshii and also examined potentiality of the RNase P proteins for bioanalytical application where precise hybridization of nucleic acids is needed.

Research Products

• [Journal Article] Crystal structure of an archaeal ribonuclease P protein Rpp38 in complex with RNA fragments containing a K-turn motif (2018)
  • Author(s): K. Oshima, X. Gao, S. Hayashi, T. Ueda, T. Nakashima, and M. Kimura
  • Journal Title: Acta Cryst. Volume: F74 Issue: 1 Pages: 57-64
  • DOI: 10.1107/s2053230x17018039
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A three-dimensional model of RNase P of the hyperthermophilic archaeon Pyrococcus horikoshii OT3 (2017)
  • Author(s): X. Gao, K. Oshima, T. Ueda, T. Nakashima, and M. Kimura
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 493 Issue: 2 Pages: 1063-1068
  • DOI: 10.1016/j.bbrc.2017.09.085
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Functional characterization of archaeal homologues of a human nuclear RNase P proteins Rpp21 and Rpp29 provides insights into the molecular basis of their cooperativity in catalysis (2017)
  • Author(s): 180.D. Jiang, K. Izumi, T. Ueda, K. Oshima, T. Nakashima, and M. Kimura
  • Journal Title: Biochem. Biophys. Res. Commun Volume: ４８２ Issue: 1 Pages: 68-74
  • DOI: 10.1016/j.bbrc.2016.10.142
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Structural basis for recognition of a kink-turn motif by an archaeal homologue of human RNase P protein Rpp38 (2016)
  • Author(s): 179.K. Oshima, Y. Kakiuchi, Y. Tanaka, T. Ueda, T. Nakashima, M. Kimura, and M. Yao
  • Journal Title: Biochem. Biophys. Res. Commun Volume: ４７４ Issue: 3 Pages: 541-546
  • DOI: 10.1016/j.bbrc.2016.04.118
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Functional implication of the heteroterameric formation of archaeal homologues of human RNase P protein pairs Pop5 with Rpp30 (2016)
  • Author(s): M. Hamasaki, K. Hazeyama, F. Iwasaki, T. Ueda, T. Nakashima, Y. Kakuta, and M. Kimura
  • Journal Title: J. Biochem Volume: １５９ Pages: 31-40
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Archaeal ribonuclease P proteins are potential for biotechnological application where a precise hybridization of nucleic acids is needed (2015)
  • Author(s): M. Miyanoshita, T. Nakashima, Y. Kakuta and M. Kimura
  • Journal Title: Biosci. Biotechnol. Biochem Volume: ７９ Pages: 2014-2017
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Mutation of the gene encoding the ribonuclease P RNA in the hyperthermophilic archaeon Thermococcus kodakarensis causes decreased growth rate and impaired processing of tRNA precursors (2015)
  • Author(s): T. Ueda, S. Ishino, K. Suematsu, T. Nakashima, Y. Kakuta, Y. Ishino, and M. Kimura
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: ４６８ Pages: 660-665
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 核酸結合タンパク質の構造機能相関と機能開発 (2017)
  • Author(s): 木村 誠
  • Organizer: 日本農芸化学会支部合同大会
• [Presentation] 超好熱性アーキアRNase P構成タンパク質のRNA活性化の構造基盤 (2015)
  • Author(s): 木村誠
  • Organizer: 日本生化学・分子生物学会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2015-12-01
• [Book] アーキア生物学 (2017)
  • Author(s): 木村 誠 等
  • Total Pages: 201
  • Publisher: 共立出版
  • ISBN: 9784320057852