| Budget Amount *help |
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
Cellulose is a major part of plant cell wall including wood, and exists as “microfibril” in which many polymer chains are packed in extended conformation. This study, on the bacterial model, aims for clarifying the mechanism of cellulose synthase protein to produce this microfibril structure.
First of all, we showed that the catalytic subunit of cellulose synthase is regularly arranged in living bacterial cell. Protein purification of cellulose synthase complex has been optimized for further higher resolution structural analysis. Observation of cellulose molecules synthesized by cellulose synthase protein was also made, and showed that cellulose molecules are aggregated through two-step process, not a single-step process.
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