Establishment of cold stress-resistance nori strains specialized for the mariculture in Tohoku in Japan

• Project/Area Number: 15H04539
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Aquatic bioproduction science
• Research Institution: Hokkaido University
• Principal Investigator: Mikami Koji 北海道大学, 水産科学研究院, 准教授 (40222319)
• Co-Investigator(Kenkyū-buntansha): 細川 雅史 北海道大学, 水産科学研究院, 教授 (10241374) ; 横山 雄彦 北里大学, 海洋生命科学部, 講師 (60296431) ; 柿沼 誠 三重大学, 生物資源学研究科, 教授 (60303757) ; 加藤 敦之 北海道大学, 理学研究院, 教授 (90177428)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000); Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2016: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: スサビノリ / ノリ養殖 / 低温耐性株 / 交雑育種 / 東北地方 / 低温ストレス / 色落ち / 育種学 / 農林水産物 / 植物 / バイオテクノロジー / 分析化学

Outline of Final Research Achievements

Tohoku area is the northern limit of the large scale-nori mariculture and has had a severe problem such as reduction of nori productivity in the early spring because of lowering temperature of the sea water, which affects negatively on seaweed growth. The present study therefore focuses on the establishment of a new strain enabling the sustainable production of nori in Tohoku area.
To establish cold stress-resistance strains, a strain whose growth tolerates to cold stress was first selected with the help from the Aichi Fisheries Research Institute. Next, the crossing of this strain with Kesen-nuna Susabi, generally used as a cultivation strain in Tohoku area, was performed and then several lines exhibiting characteristics different from both parental strains were finally isolated. Thus, it is necessary to confirm growth under cold stress conditions of these new strains, which could enable to establish novel strains that are adapting to mariculture in Tohoku area in near future.

Research Products

• [Journal Article] The presence of free D-aspartate in marine macroalgae is restricted to the Sargassaceae family (2018)
  • Author(s): Yokoyama Takehiko、Tokuda Masaharu、Amano Masafumi、Mikami Koji
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 82 Issue: 2 Pages: 268-273
  • DOI: 10.1080/09168451.2017.1423228
  • NAID: 120006547282
  • Peer Reviewed
• [Journal Article] Parthenosporophytes of the brown alga Ectocarpus siliculosus exhibit sex-dependent differences in thermotolerance as well as fatty acid and sterol composition (2018)
  • Author(s): Mikami, K., Ito, M., Taya, K., Kishimoto, I., Kobayashi, T., Itabashi, Y. and Tanaka, R.
  • Journal Title: Mar. Environ. Res. Volume: 136 Pages: 188-195
  • DOI: 10.1016/j.marenvres.2018.02.003
  • NAID: 120006847753
  • Peer Reviewed
• [Journal Article] Discolored red seaweed Pyropia yezoensis with low commercial value is a novel resource for production of agar polysaccharides (2018)
  • Author(s): Sasuga, K., Yamanashi, T., Nakayama, S., Ono, S. and Mikami, K.
  • Journal Title: Mar. Biotechnol. Volume: 20 Issue: 4 Pages: 520-530
  • DOI: 10.1007/s10126-018-9823-7
  • NAID: 120006709285
  • Peer Reviewed
• [Journal Article] Dietary supplementation of red alga Pyropia spheroplasts on growth, feed utilization and body composition of sea cucumber, Apostichopus japonicas (Selenla) (2017)
  • Author(s): Shahabuddin, A.M., Khan, M., Mikami, K., Araki, T. and Yoshimatsu, T.
  • Journal Title: Aquac. Res. Volume: 48 Issue: 10 Pages: 5363-5372
  • DOI: 10.1111/are.13350
  • Peer Reviewed
• [Journal Article] Phytohormones in red seaweeds: a technical review of methods for analysis and a consideration of genomic data (2017)
  • Author(s): Mori, C.I. et al.
  • Journal Title: Botanica Marina Volume: 60 Issue: 2 Pages: 153-170
  • DOI: 10.1515/bot-2016-0056
  • Peer Reviewed
• [Journal Article] Optimization of yield and quality of agar polysaccharide isolated from the marine red macroalga Pyropia yezoensis (2017)
  • Author(s): Sasuga, K., Yamanashi, T., Nakayama, S., Ono S. and Mikami, K.
  • Journal Title: Algal Res. Volume: 26 Pages: 123-130
  • DOI: 10.1016/j.algal.2017.07.010
  • NAID: 120006716310
  • Peer Reviewed
• [Journal Article] Simultaneous determination of primary and secondary D- and L-amino acids by reversed-phase high-performance liquid chromatography using pre-column derivatization with two-step labelling method (2017)
  • Author(s): Yokoyama Takehiko、Tokuda Masaharu、Amano Masafumi、Mikami Koji
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 81 Issue: 9 Pages: 1681-1686
  • DOI: 10.1080/09168451.2017.1340090
  • NAID: 120006482573
  • Peer Reviewed
• [Journal Article] Selection and functional analysis of a Pyropia yezoensis ammonium transporter PyAMT1 in potassium deficiency (2017)
  • Author(s): Adams, E., Mikami, K. and Shin, R.
  • Journal Title: J. Appl. Phycol. Volume: 29 Issue: 5 Pages: 2617-2626
  • DOI: 10.1007/s10811-017-1196-1
  • NAID: 120006523717
  • Peer Reviewed
• [Journal Article] Oxidative stress promotes asexual reproduction and apogamy in the red seaweed Pyropia yezoensis. (2017)
  • Author(s): Takahashi, M. and Mikami, K.
  • Journal Title: Front. Plant Sci. Volume: 8 Pages: 62-62
  • DOI: 10.3389/fpls.2017.00062
  • NAID: 120005952029
  • Peer Reviewed / Open Access
• [Journal Article] Dietary supplementation of red alga Pyropia spheroplasts on growth, feed utilization and body composition of sea cucumber, Apostichopus japonicas (Selenla). (2017)
  • Author(s): Shahabuddin, A.M., Khan, M., Mikami, K., Araki, T. and Yoshimatsu, T.
  • Journal Title: Aquaculture Res. Volume: 48
  • Peer Reviewed
• [Journal Article] Isolation and characterization of a new DUR3-like gene, PyDUR3.3, from the marine macroalga Pyropia yezoensis (Rhodophyta) (2016)
  • Author(s): Makoto Kakinuma，Kohei Suzuki，Shintaro Iwata，Daniel A. Coury，Shouei Iwade，Koji Mikami
  • Journal Title: Fisheries Science Volume: 82 Issue: 1 Pages: 171-184
  • DOI: 10.1007/s12562-015-0947-7
  • NAID: 120006347190
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Comprehensive quantification and genome survey reveal the presence of novel phytohormone action modes in red seaweeds (2016)
  • Author(s): Mikami, K., Mori, I.C., Matsuura, T., Ikeda, Y., Kojima, M., Sakakibara, H., Hirayama, T.
  • Journal Title: J. Applied Phycology Volume: 未定 Issue: 4 Pages: 1-10
  • DOI: 10.1007/s10811-015-0759-2
  • NAID: 120006329010
  • Peer Reviewed
• [Journal Article] Phototropism in the marine red macroalga Pyropia yezoensis. (2016)
  • Author(s): Takahashi, M. and Mikami, K.
  • Journal Title: Am. J. Plant Sci. Volume: 7 Issue: 17 Pages: 2412-2428
  • DOI: 10.4236/ajps.2016.717211
  • NAID: 120005898507
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Characterization of an eukaryotic PL-7 alginate lyase in the marine red alga Pyropia yezoensis. (2016)
  • Author(s): Inoue, A., Mashino, C., Uji, T., Saga, N., Mikami, K. and Ojima, T.
  • Journal Title: Current Biotechnology Volume: 4 Issue: 3 Pages: 240-248
  • DOI: 10.2174/2211550104666150915210434
  • Peer Reviewed / Open Access
• [Journal Article] Chemoprotective effects of a recombinant protein from Pyropia yezoensis and synthetic peptide against acetaminophen-induced Chang liver cell death. (2015)
  • Author(s): Choi, Y.H., Kim, E.-Y., Mikami, K. and Nam, T.J.
  • Journal Title: International Journal of Molecular Medicine Volume: 36 Issue: 2 Pages: 369-376
  • DOI: 10.3892/ijmm.2015.2253
  • NAID: 120005859733
  • Peer Reviewed / Int'l Joint Research
• [Presentation] 原始紅藻スサビノリにおける胞子体から配偶体への移行は減数分裂に依存していない (2018)
  • Author(s): 三上浩司、李成澤、濱洋一郎
  • Organizer: 第59回日本植物生理学会年会
• [Presentation] 海産紅藻スサビノリにおけるオーキシンによる先端生長の制御 (2017)
  • Author(s): 田谷賢祐、林謙一郎、三上浩司
  • Organizer: 日本植物生理学会
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] Contribution of an ammonium transporter To Na+/K+ homeostasis in marine red alga Pyropia yezoensis (2017)
  • Author(s): Eri Adams, Koji Mikami, Ryoung Shin
  • Organizer: 日本植物生理学会
  • Place of Presentation: 鹿児島大学郡元キャンパス（鹿児島県鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] Heat stress memory is responsible for acquisition of thermotolerance in the red seaweed Bangia fuscopurpurea (2017)
  • Author(s): Mikami, K. and Kishimoto, I.
  • Organizer: 11th International Phycological Congress
  • Int'l Joint Research
• [Presentation] Nitrogen-related transporters at work on potassium deficiency and salt tolerance in Pyropis yezoensis (2017)
  • Author(s): Adams, E., Shin, R. and Mikami, K.
  • Organizer: 11th International Phycological Congress
  • Int'l Joint Research
• [Presentation] 原始紅藻類は熱ストレスを記憶することで高温耐性を獲得する (2017)
  • Author(s): 三上浩司
  • Organizer: 第19回マリンバイオテクノロジー学会大会
  • Invited
• [Presentation] 産業廃棄物の有効利用に向けた色落ちノリからの寒天抽出法の確立 (2017)
  • Author(s): 流石啓司、山梨智也、中山滋、小野秀悦、三上浩司
  • Organizer: 第19回マリンバイオテクノロジー学会大会
• [Presentation] オーキシンによる紅藻スサビノリ糸状体の先端生長制御 (2017)
  • Author(s): 田谷賢祐、三上浩司
  • Organizer: 2017年度北海道植物学会大会
• [Presentation] 原始紅藻ウシケノリにおけるストレス記憶に基づく高温耐性能獲得 (2017)
  • Author(s): 岸本育也、三上浩司
  • Organizer: 2017年度北海道植物学会大会
• [Presentation] Is “stress memory” responsible for acquired heat tolerance present in red seaweeds? (2016)
  • Author(s): Koji Mikami
  • Organizer: 7th Bilateral Seminar Italy-Japan, Physiological and chemical impacts on marine organisms: emerging hazards and strategies for saving the ocean
  • Place of Presentation: Minamiboso Tomiura Royal Hotel, Minamiboso, Chiba, Japan
  • Year and Date: 2016-11-29
  • Int'l Joint Research / Invited
• [Presentation] Carotenoid content and biosynthesis pathway in a red alga, Pyropia yezoensis. (2016)
  • Author(s): Koizumi, J., Kobayashi, N., Mikami, K., Maoka, T., Miayashita, K. and Hosokawa, M.
  • Organizer: SNFF2016 (The International Society for Nutraceuticals & Functional Foods 2016)
  • Place of Presentation: Rosen Shingle Creek Hotel, Orlando, FL, USA
  • Year and Date: 2016-10-08
  • Int'l Joint Research
• [Presentation] 海産原始紅藻ウシケノリにおける高温ストレス記憶の研究 (2016)
  • Author(s): 岸本育也、須田昌宏、三上浩司
  • Organizer: 日本水産学会
  • Place of Presentation: 近畿大学農学部奈良キャンパス（奈良県奈良市）
  • Year and Date: 2016-09-08
• [Presentation] ホンダワラ属海藻のD-アスパラギン酸 (2016)
  • Author(s): 横山雄彦、高田洸輔、戸田航洋、難波信由、徳田雅治、三上浩司
  • Organizer: 日本水産学会
  • Place of Presentation: 近畿大学農学部奈良キャンパス（奈良県奈良市）
  • Year and Date: 2016-09-08
• [Presentation] 紅藻スサビノリに含まれるカロテノイドの定量と生合成経路に関する研究 (2016)
  • Author(s): 小泉次郎、小林乗時、三上浩司、眞岡孝至、宮下和夫、細川雅史
  • Organizer: 日本油化学会
  • Place of Presentation: 奈良女子大学（奈良県奈良市）
  • Year and Date: 2016-09-07
• [Presentation] 紅藻スサビノリのカロテノイド生合成経路の解析 (2016)
  • Author(s): 細川雅史、小林乗時、小泉次郎、三上浩司、宮下和夫、山野由美子、和田昭盛
  • Organizer: カロテノイド研究談話会
  • Place of Presentation: ホテルムーンビーチ 4F 酒座参番（沖縄県国頭郡恩納村）
  • Year and Date: 2016-06-24
• [Presentation] アマノリ類の生理制御機構を理解するための２つの視点 (2016)
  • Author(s): 三上浩司
  • Organizer: マリンバイオテクノロジー学会
  • Place of Presentation: 北海道大学函館キャンパス（北海道函館市）
  • Year and Date: 2016-05-28
  • Invited
• [Presentation] 海産紅藻ウシケノリBangia fuscopurpureaにおけるストレス記憶による高温耐性能の獲得 (2016)
  • Author(s): 岸本育也、須田昌宏、三上浩司
  • Organizer: 日本応用藻類学会
  • Place of Presentation: 東京海洋大学（東京都品川区）
  • Year and Date: 2016-05-14
• [Presentation] 海藻生物学の方向性 (2016)
  • Author(s): 三上浩司
  • Organizer: 日本応用藻類学会
  • Place of Presentation: 東京海洋大学（東京都品川区）
  • Year and Date: 2016-05-14
  • Invited
• [Presentation] 褐藻シオミドロの雄性配偶体に偏ったサイトカイニンの蓄積 (2015)
  • Author(s): 三上浩司、池田陽子、松浦恭和、森泉、平山隆志
  • Organizer: 平成27年度日本水産学会秋季大会
  • Place of Presentation: 東北大学 （宮城県、仙台市）
  • Year and Date: 2015-09-22
• [Presentation] 海藻は未知の植物ホルモン情報伝達機構を持つ (2015)
  • Author(s): 三上浩司、森泉、松浦恭和、池田陽子、平山隆志
  • Organizer: 日本応用藻類学会第14回大会
  • Place of Presentation: 東京海洋大学 （東京都、品川区）
  • Year and Date: 2015-05-16
• [Remarks] 三上浩司
  • URL: http://www2.fish.hokudai.ac.jp/faculty-member/mikami-kohji