Role of autophagy-lysosomal related responses on uterine modulation at the time of maternal-fetal recogntion
| Project/Area Number |
15H04579
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Animal production science
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山内 伸彦 九州大学, 農学研究院, 准教授 (00363325)
木村 康二 岡山大学, 環境生命科学研究科, 教授 (50355070)
川原 学 北海道大学, 農学研究院, 准教授 (70468700)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2017: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2015: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Keywords | 妊娠認識 / オートファジー・リソソーム / ウシ / インターフェロンτ / ウシ子宮 / カテプシン / 妊娠 / アポトーシス / ピロトーシス / IFNτ / 妊娠応答 / IFNtau / IFNAR / 畜産学 / 子宮 |
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| Outline of Final Research Achievements |
In the present study, we detected the activation of cathepsin (CTS)B in the pregnant bovine uterine tissue. This activation was thought to be stimulated by interferon tau(IFN)T secreted by embryo. Although expression of CTS genes was detected by pregnant and IFNT-treated non pregnant uterine tissue, expression of autophagy related genes was not changed. These results suggest the activation of lysosome and lysosomal CTS is triggered by IFNT for uterine modulation, but contribution of autophagy is low. Further study is needed to clarify the roles of autophagy. RNA interference of each subunit construction of type I IFN receptor subunit (IFNAR1, R1) by siRNA revealed that IFNT stimulated signal transduction is dominantly affected by IFNAR1 for inducing IFN related gene expressions. In addition, high levels of pregnancy specific gene expression were detected in the cervical tissue that is directly connected with uterus for the first time.
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Estrous cycle stage-dependent manner of type I interferon-stimulated genes induction in the bovine endometrium2017
Author(s)
Shirozu T, Iwano H, Ogiso T, Suzuki T, Balboula AZ, Bai H, Kawahara M, Kimura K, Takahashi H, Rulan B, Kim SW, Yanagawa Y, Nagano M, Imakawa K, Takahashi M.
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Journal Title
Journal of Reproduction and Development
Volume: 63
Issue: 3
Pages: 211-220
DOI
NAID
ISSN
0916-8818, 1348-4400
Related Report
Peer Reviewed / Int'l Joint Research
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