Does prostaglandin become the insect gwowth control factor?
| Project/Area Number |
15H04611
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Insect science
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| Research Institution | Kyushu University |
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Principal Investigator |
Yamamoto Koji 九州大学, 農学研究院, 助教 (00346834)
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| Co-Investigator(Kenkyū-buntansha) |
坪田 拓也 国立研究開発法人農業生物資源研究所, その他部局等, 主任研究員 (00612772)
有竹 浩介 第一薬科大学, 薬学部, 教授 (70390804)
東浦 彰史 大阪大学, たんぱく質研究所, 助教 (90598129)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2015: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
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| Keywords | プロスタグランジン / プロスタグランジン合成酵素 / カイコ / 酵素 / 昆虫 |
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| Outline of Final Research Achievements |
Knowledge on role and synthesis of prostaglandins (PGs) in insect are limited. PGE synthase (PGES) and PGD synthase (PGDS) were already identified, and three-dimensional structure of PGES was determined.
In this study, we examined physiological role of PG by using genome-editing, TALEN, and X-ray analysis. In knock-out mutant of PGES, PG was not present by using mass-spectrometry. Since we were interested in composition of the active sites in PGDS, we determined crystal structure of PGDS. Based on the PGDS structure and site-directed mutagenesis, we identified the amino acid residues (Tyr8、 Lue14、Trp39、Lys43、Gln50、Val51、Gln63、and Ser64) involved in catalytic function.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] Wide distribution of a major East Asian noctuid pest explained by genomic adaptation to polyphagy and insecticides2017
Author(s)
Cheng T., Wu J., Wu Y., Chilukuri R., Huang L., Yamamoto K., et al, Arunkumar A., Kishino H., Goldsmith M., Feng Q., Xia Q., and Mita K.
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Journal Title
Nature Ecology & Evolution
Volume: 1
Pages: 1747-1756
Related Report
Peer Reviewed / Int'l Joint Research
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