Control of freezing of water at subzero temperatures in woody plant cells
| Project/Area Number |
15H04615
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental agriculture(including landscape science)
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| Research Institution | Hokkaido University |
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Principal Investigator |
Arakawa Keita 北海道大学, 農学研究院, 准教授 (00241381)
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| Co-Investigator(Kenkyū-buntansha) |
福士 幸治 北海道大学, 農学研究院, 准教授 (60218906)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Keywords | 氷核細菌 / 氷核タンパク質 / ポリフェノール / 氷核活性 / 氷核形成阻害活性 / 過冷却 / inaA / 抗氷核ポリフェノール / 氷核形成阻害 |
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| Outline of Final Research Achievements |
It is well known that existence of the ice nucleation substances (ice nucleators) such as silver iodide and ice nucleation bacteria promote freezing of supercooled water. Because these substances catalyze freezing of water, ice nucleation activity (INA) increases freezing temperature of the solution. On the other hand, recent studies showed that some polyphenols repressed INA of ice nucleators and depressed freezing temperature of the solution containing ice nucleators. Existence of these polyphenols (anti-INA polyphenols) maintained supercooling state of the solution. For study on the anti-INA process by anti-INA polyphenols, His-inaA was highly purified from the soluble fraction prepared from the crude extracts of Escherichia coli cells overexpressing His-inaA and soluble form of His-inaA was characterized. INA was detected in the soluble form of His-inaA and repressed by addition of some anti-INA polyphenols.
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Report
(4 results)
Research Products
(29 results)
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[Presentation] 形質転換樹木の超低温保存技術に関する基礎研究2016
Author(s)
川村浩平、嘉見大助、鈴木伸吾、田中大介、遠藤圭太、山岸祐介、荒川圭太
Organizer
Cryopreservation Conference 2016
Place of Presentation
自然科学研究機構・岡崎コンファレンスセンター（愛知県・岡崎市）
Year and Date
2016-11-10
Related Report
Invited
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[Presentation] 樹木由来の氷核活性の検出2015
Author(s)
鉄穴口晃、鈴木伸吾、荒川圭太
Organizer
日本木材学会北海道支部 平成27年度研究発表会
Place of Presentation
旭川地場産業振興センター（北海道旭川市）
Year and Date
2015-11-13
Related Report
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[Presentation] 交雑ポプラの超低温保存に関する研究2015
Author(s)
川村浩平、嘉見大助、鈴木伸吾、桜井健至、荒川圭太
Organizer
日本木材学会北海道支部 平成27年度研究発表会
Place of Presentation
旭川地場産業振興センター（北海道旭川市）
Year and Date
2015-11-13
Related Report
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