Analysis of molecular mechanisms of retinal neuron development

• Project/Area Number: 15H04669
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Osaka University
• Principal Investigator: Furukawa Takahisa 大阪大学, たんぱく質研究所, 教授 (50260609)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: 網膜 / 細胞分化 / 視覚 / 転写因子 / エピジェネテティック / 視細胞 / 分化 / エピジェネティック / ヒストン修飾 / 脳・神経 / 発生・分化 / 神経科学 / 遺伝子 / 神経 / ニューロン / 網膜変性 / 再生

Outline of Final Research Achievements

We have studied molecular mechanisms underlying retinal photoreceptor development, synapse formation, and retinal degeneration. In the current study, as a model system to study vertebrate central nervous system development, we have analyzed cell fate determination of retinal photoreceptor cells. We found that transcriptional regulation and epigenetic mechanisms play crucial roles in retinal neuronal differentiation and maturation.

Research Products

• [Int'l Joint Research] KAIST(韓国)
• [Journal Article] Lrit1, a Retinal Transmembrane Protein, Regulates Selective Synapse Formation in Cone Photoreceptor Cells and Visual Acuity (2018)
  • Author(s): Ueno Akiko、Omori Yoshihiro、Sugita Yuko、Watanabe Satoshi、Chaya Taro、Kozuka Takashi、Kon Tetsuo、Yoshida Satoyo、Matsushita Kenji、Kuwahara Ryusuke、Kajimura Naoko、Okada Yasushi、Furukawa Takahisa
  • Journal Title: Cell Reports Volume: 22 Issue: 13 Pages: 3548-3561
  • DOI: 10.1016/j.celrep.2018.03.007
  • Peer Reviewed / Open Access
• [Journal Article] Metabolic shift induced by systemic activation of T cells in PD-1-deficient mice perturbs brain monoamines and emotional behavior (2017)
  • Author(s): Miyajima M、Zhang B、Sugiura Y、Sonomura K、Guerrini MM、Tsutsui Y、Maruya M、Vogelzang A、Chamoto K、Honda K、Hikida T、Ito S、Qin H、Sanuki R、Suzuki K、Furukawa T、Ishihama Y、Matsuda F、Suematsu M、Honjo T、Fagarasan S
  • Journal Title: Nature Immunology Volume: 18 Issue: 12 Pages: 1342-1352
  • DOI: 10.1038/ni.3867
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Samd7 is a cell type-specific PRC1 component essential for establishing retinal rod photoreceptor identity (2017)
  • Author(s): Omori Yoshihiro、Kubo Shun、Kon Tetsuo、Furuhashi Mayu、Narita Hirotaka、Kominami Taro、Ueno Akiko、Tsutsumi Ryotaro、Chaya Taro、Yamamoto Haruka、Suetake Isao、Ueno Shinji、Koseki Haruhiko、Nakagawa Atsushi、Furukawa Takahisa
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 114 Issue: 39
  • DOI: 10.1073/pnas.1707021114
  • Peer Reviewed / Open Access
• [Journal Article] The TRPM1 Channel Is Required for Development of the Rod ON Bipolar Cell-AII Amacrine Cell Pathway in the Retinal Circuit (2017)
  • Author(s): Kozuka Takashi、Chaya Taro、Tamalu Fuminobu、Shimada Mariko、Fujimaki-Aoba Kayo、Kuwahara Ryusuke、Watanabe Shu-Ichi、Furukawa Takahisa
  • Journal Title: The Journal of Neuroscience Volume: 37 Issue: 41 Pages: 9889-9900
  • DOI: 10.1523/jneurosci.0824-17.2017
  • Peer Reviewed / Open Access
• [Journal Article] Versatile functional roles of horizontal cells in the retinal circuit (2017)
  • Author(s): Chaya Taro、Matsumoto Akihiro、Sugita Yuko、Watanabe Satoshi、Kuwahara Ryusuke、Tachibana Masao、Furukawa Takahisa
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 5540-5540
  • DOI: 10.1038/s41598-017-05543-2
  • Peer Reviewed / Open Access
• [Journal Article] A modulatory role of the Rax homeobox gene in mature pineal gland function: Investigating the photoneuroendocrine circadian system of a Rax conditional knockout mouse (2017)
  • Author(s): Rohde Kristian、Bering Tenna、Furukawa Takahisa、Rath Martin Fredensborg
  • Journal Title: Journal of Neurochemistry Volume: 143 Issue: 1 Pages: 100-111
  • DOI: 10.1111/jnc.14120
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Regulation of bone mass through pineal-derived melatonin-MT2 receptor pathway (2017)
  • Author(s): Sharan Kunal、Lewis Kirsty、Furukawa Takahisa、Yadav Vijay K.
  • Journal Title: Journal of Pineal Research Volume: 63 Issue: 2
  • DOI: 10.1111/jpi.12423
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Critical involvement of ZEB2 in collagen fibrillogenesis: the molecular reminiscent of Mowat-Wilson syndrome with Ehlers-Danlos syndrome. (2017)
  • Author(s): Teraishi M, Takaishi M, Nakajima K, Ikeda M, Higashi Y, Shimoda S, Asada Y, Hijikata A, Ohara O, Hirai Y, Mizuno S, Fukada T, Furukawa T, Wakamatsu N, Sano S.
  • Journal Title: Scientific Report Volume: 7:46565 Issue: 1 Pages: 1-10
  • DOI: 10.1038/srep46565
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ick ciliary kinase is essential for planar cell polarity formation in inner ear hair cells and hearing function. (2017)
  • Author(s): Okamoto S, Chaya T, Omori Y, Kuwahara R, Kubo S, Sakaguchi H, Furukawa T
  • Journal Title: Journal of Neuroscience Volume: 37 Issue: 8 Pages: 2073-2085
  • DOI: 10.1523/jneurosci.3067-16.2017
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Loss of ift122, a Retrograde Intraflagellar Transport (IFT) Complex Component, Leads to Slow, Progressive Photoreceptor Degeneration Due to Inefficient Opsin Transport. (2016)
  • Author(s): Boubakri M, Chaya T, Hirata H, Kajimura N, Kuwahara R, Ueno A, Malicki J, Furukawa T, Omori Y
  • Journal Title: The Journal of Biological Chemistry Volume: 291 Issue: 47 Pages: 24465-24474
  • DOI: 10.1074/jbc.m116.738658
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Temporal Properties of Cone ERGs of Pikachurin Null Mutant Mouse. (2016)
  • Author(s): 1.Kominami T, Ueno S, Nakanishi A, Kominami A, Kondo M, Furukawa T, Terasaki H.
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 57 Issue: 3 Pages: 1264-1269
  • DOI: 10.1167/iovs.15-17708
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Response: Commentary: "Prdm13 regulates subtype specification of retinal amacrine interneurons and modulates visual sensitivity" (2016)
  • Author(s): Sugita Y, Watanabe S, Furukawa T
  • Journal Title: Front Cell Neurosci Volume: 9 Pages: 520-520
  • DOI: 10.3389/fncel.2015.00520
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Prdm13 regulates subtype specification of retinal amacrine interneurons and modulates visual sensitivity. (2015)
  • Author(s): Watanabe S, Sanuki R, Sugita Y, Imai W, Yamazaki R, Kozuka T, Ohsuga M, Furukawa T
  • Journal Title: J Neurosci Volume: 35 Issue: 20 Pages: 8004-20
  • DOI: 10.1523/jneurosci.0089-15.2015
  • Peer Reviewed / Open Access
• [Journal Article] Rax Homeoprotein Regulates Photoreceptor Cell Maturation and Survival in Association with Crx in the Postnatal Mouse Retina (2015)
  • Author(s): Irie S, Sanuki R, Muranishi Y, Kato K, Chaya T, Furukawa T
  • Journal Title: Mol Cell Biol Volume: 35 Issue: 15 Pages: 2583-2596
  • DOI: 10.1128/mcb.00048-15
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Mef2d is essential for the maturation and integrity of retinal photoreceptor and bipolar cells. (2015)
  • Author(s): Omori Y, Kitamura T, Yoshida S, Kuwahara R, Chaya T, Irie S, Furukawa T.
  • Journal Title: Genes to Cells Volume: 印刷中 Issue: 5 Pages: 408-26
  • DOI: 10.1111/gtc.12233
  • Peer Reviewed / Open Access
• [Presentation] ロイシンリッチ膜蛋白質Lrit1は網膜の視細胞ー双極細胞のシナプス形成を制御する (2018)
  • Author(s): 古川貴久, 上野明希子, 岡田康志
  • Organizer: 第95回日本生理学会大会
  • Invited
• [Presentation] Role of TRPM1 channel in retinal circuit development (2017)
  • Author(s): Takashi Kozuka, Taro Chaya, Takahisa Furukawa
  • Organizer: オーストラリア国立大学・蛋白研合同シン ポジウムIPR/RSC Symposium
  • Int'l Joint Research
• [Presentation] SAMドメイン蛋白質Samd7は網膜桿体視細胞の最終分化をエピジェネティックに制御する組織特異的なポリコーム複合体１の構成要素である (2017)
  • Author(s): 茶屋太郎, 小塚孝司, 田丸文信, 島田真理子, 桑原隆亮, 渡辺修一, 古川貴久
  • Organizer: 2017年度生命科学系学会合同年次大会
• [Presentation] ロイシンリッチリピート膜蛋白質による網膜視細胞のシナプス形成機構 (2017)
  • Author(s): 上野明希子, 古川貴久
  • Organizer: 2017年度生命科学系学会合同年次大会
  • Invited
• [Presentation] TRPM1チャネルの網膜神経回路発達における役割 (2017)
  • Author(s): 茶屋太郎, 小塚孝司, 田丸文信, 島田真理子, 桑原隆亮, 渡辺修一, 古川貴久
  • Organizer: 2017年度生命科学系学会合同年次大会
• [Presentation] 桿体視細胞のアイデンティティを制御するエピジェネティックな遺伝子発現制御機構 (2017)
  • Author(s): 久保 竣, 古川貴久
  • Organizer: 第10回Retina Research Meeting （RRM）
• [Presentation] 水平細胞は網膜神経回路において多様な機能を有する (2017)
  • Author(s): 古川貴久、茶屋太郎、松本彰弘、杉田祐子、桒原隆亮、立花政夫
  • Organizer: 第94回日本生理学会大会
  • Place of Presentation: アクトシティ浜松（静岡県浜松市）
• [Presentation] 膜神経回路における水平細胞の生理機能の解析 (2016)
  • Author(s): 茶屋太郎、松本彰弘、杉田祐子、桒原隆亮、立花政夫、古川貴久
  • Organizer: 第9回RRM（Retina Research Meeting）
  • Place of Presentation: JPタワーホール&カンファレンス（東京都千代田区）
• [Presentation] Role of TRPM1 Channel in Retinal Circuit Development (2016)
  • Author(s): Takashi Kozuka, Mariko Shimada Fuminobu Tamalu, Taro Chaya, Shuhei Mikusa, Takahisa Furukawa
  • Organizer: ISER2016
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Int'l Joint Research / Invited
• [Presentation] Transcriptional Regulation of Photoreceptor Cell Development and Maturation (2016)
  • Author(s): Takahisa Furukawa, Yoshihiro Omori, Shun Kubo, Mayu Furuhashi
  • Organizer: ISER2016
  • Place of Presentation: 京王プラザホテル（東京都新宿区）
  • Int'l Joint Research / Invited
• [Presentation] 水平細胞の選択的破壊による網膜神経節細胞での視覚情報の符号化への影響 (2016)
  • Author(s): 松本彰弘、茶屋太郎、杉田祐子、渡邉哲史、桒原隆亮、立花政夫、古川貴久
  • Organizer: 第20回視覚科学フォーラム
  • Place of Presentation: 大阪大学脳情報通信融合研究センター（大阪府吹田市）
• [Presentation] エピジェネティックな遺伝子発現制御機構による網膜視細胞の成熟の制御 (2016)
  • Author(s): 久保竣、大森義裕、古橋真佑、上野明希子、茶屋太郎、上野真治、古川貴久
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
• [Presentation] 網膜神経回路における水平細胞の予想外の機能 Unexpected Functional Roles of Horizontal Cells in the Retinal Circuit (2016)
  • Author(s): 茶屋太郎、松本彰弘、杉田祐子、渡邉哲史、桒原隆亮、立花政夫、古川貴久
  • Organizer: 第39回 日本神経科学大会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
• [Presentation] 網膜視細胞の成熟における転写因子Mef2dの機能と視細胞変性 (2016)
  • Author(s): 上野明希子、大森義裕、北村民樹、茶屋太郎、古川貴久
  • Organizer: 第63回 日本生化学会 近畿支部例会
  • Place of Presentation: 神戸薬科大学（兵庫県神戸市）
• [Presentation] DO3 Prdm13による網膜のアマクリン細胞サブタイプ分化の制御と資格応答感度調整 (2016)
  • Author(s): 久保竣、杉田祐子、小塚孝司、古川貴久
  • Organizer: 第63回 日本生化学会 近畿支部例会
  • Place of Presentation: 神戸薬科大学（兵庫県神戸市）
• [Presentation] Unexpected functional roles of horizontal cells in the retinal circuit (2016)
  • Author(s): Taro Chaya, Akihiro Matsumoto, Yuko Sugita, Satoshi Watanabe, Ryusuke Kuwahara, Masao Tachibana, Takahisa Furukawa
  • Organizer: ARVO2016
  • Place of Presentation: Seattle（USA）
  • Int'l Joint Research
• [Presentation] 網膜視細胞と双極細胞シナプス成熟における転写因子Mef2dの機能と視細胞変性 (2015)
  • Author(s): 上野明希子、大森義裕、古川貴久
  • Organizer: 第8回 Retina Research Meeting
  • Place of Presentation: JPタワー ホール&カンファレンス（東京都・千代田区）
  • Year and Date: 2015-10-31
• [Presentation] 抑制性の転写因子Prdm13は網膜アマクリン細胞のサブタイプ分化と光応答感度を制御する (2015)
  • Author(s): 小塚孝司、杉田祐子、古川貴久
  • Organizer: 視覚科学フォーラム第19回研究会
  • Place of Presentation: ホテル福島グリーンパレス（福島県・福島市）
  • Year and Date: 2015-08-19
• [Presentation] Essential roles of Prdm13 in subtype specification of retinal amacrine interneurons and modulation of visual sensitivity (2015)
  • Author(s): Takahisa Furukawa, Yuko Sugita, Takashi Kozuka, Satoshi Watanabe
  • Organizer: World Congress on Ocular Cell and Regenerative Biology and the 6th National Forum of Graduate, Students in Ophthalmology
  • Place of Presentation: 広州（中国）
  • Year and Date: 2015-08-07
  • Int'l Joint Research
• [Presentation] 脳の発生と機能におけるﾏｲｸﾛRNA‐124aの役割 Essential roles of miR-124a in brain development and function, microRNA and epigenetic changing in CNS (2015)
  • Author(s): 古川貴久、佐貫理佳子
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-07-30
• [Presentation] 幹細胞、網膜アマクリン細胞のサブタイプ分化と視覚応答の解析, An analysis of molecular mechanisms underlying subtype specification of retinal amacrine interneurons and their functional roles, in vision (2015)
  • Author(s): 小塚孝司、渡邉哲史、佐貫理佳子、杉田祐子、今井航、山崎竜史、大菅瑞樹、古川貴久
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2015-07-28
• [Presentation] Protein 4.1G-mediated membrane trafficking is essential for correct rod synaptic location in the retina and for normal visual function (2015)
  • Author(s): Takahisa Furukawa, Rikako Sanuki, Satoshi Watanabe, Yuko Sugita, Shoichi Irie, Takashi Kozuka, Mariko Shimada, Shinji Ueno, Jiro Usukura
  • Organizer: The Association for Research in Vision and Ophthalmology (ARVO) 2015
  • Place of Presentation: デンバー（アメリカ）
  • Year and Date: 2015-05-04
  • Int'l Joint Research
• [Book] Encyclopeia of Signaling Molecules (2018)
  • Author(s): Tetsuo Kon, Takahisa Furukawa
  • Publisher: Springer
  • ISBN: 9783319671994
• [Book] 光と生命の事典 (2016)
  • Author(s): 古川貴久 他191名
  • Total Pages: 436
  • Publisher: 朝倉書店
• [Remarks] 大阪大学蛋白質研究所 分子発生学研究室
  • URL: http://www.protein.osaka-u.ac.jp/furukawa_lab/
• [Remarks] 大阪大学蛋白質研究所 分子発生学研究室ホームページ
  • URL: http://www.protein.osaka-u.ac.jp/furukawa_lab/