Hierarchical self-organization of circadian system: synchronization of cellular oscillators and pacemaker coupling
| Project/Area Number |
15H04679
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology(including physical medicine and nutritional physiology)
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| Research Institution | Hokkaido University |
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Principal Investigator |
HONMA Sato 北海道大学, 脳科学研究教育センター, 招へい教員 (20142713)
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| Co-Investigator(Kenkyū-buntansha) |
榎木 亮介 北海道大学, 電子科学研究所, 准教授 (00528341)
小野 大輔 名古屋大学, 環境医学研究所, 助教 (30634224)
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| Co-Investigator(Renkei-kenkyūsha) |
TOKUDA Isao 立命館大学, 理工学部, 教授 (00261389)
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| Research Collaborator |
MIEDA Michihiro
OHMIYA Yoshihiro
NAKAJIMA Yoshihiro
KURODA Shigeru
TAKAGI Seiji
YOSHIKAWA Tomoko
ODA Yoshiaki
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2015: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | 生体リズム / 時計遺伝子 / 視交叉上核 / イメージング / 結合振動子 / 概日リズム |
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| Outline of Final Research Achievements |
We aimed to clarify the self-organizing mechanisms for the hierarchical multi-oscillator structure of the mammalian circadian clock in the suprachiasmatic nucleus (SCN) using knockout mice for clock genes and neuropeptides functions, luciferase reporter mice for continuous monitoring clock gene expression, and fluorescence sensors for monitoring intracellular calcium and membrane potentials. We demonstrated that both VIP and vasopressin act as synchronizers for cellular rhythms. We also found that their roles change depending on postnatal development, in which clock gene Cry is involved. We also found that GABA in the SCN acts not in cellular rhythm generation but in coherent neural rhythm output from the SCN to make the central circadian clock free of bursts. We further found that Per1 rhythms in the discrete areas in the SCN are involved in transferring photoperiodic signals to behavior rhythm output by changing the phase relations.
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Report
(4 results)
Research Products
(42 results)
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[Journal Article] Dissociation of Per1 and Bmal1 circadian rhythms in the suprachiasmatic nucleus in parallel with behavioral outputs.2017
Author(s)
Ono, D., Honma, S., Nakajima, Y., Kuroda, S., Enoki, R., and Honma, K.
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Journal Title
Proc. Natl. Acad. Sci. U S A.
Volume: 114
Issue: 18
Pages: 3699-3708
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Coupling controls synchrony of clock cells in development and knockouts2015
Author(s)
Tokuda, I., Ono, D., Ananthasubramaniam, B., Honma, S., Honma, K., and Herzel, H.
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Journal Title
Biophysical Journal
Volume: 109
Issue: 10
Pages: 2159-2170
DOI
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Peer Reviewed / Int'l Joint Research
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[Book] Biological Clocks2015
Author(s)
Honma S, Ono D, Enoki R, Yoshikawa T, Kuroda S and Honma K
Total Pages
253
Publisher
Hokkaido Univ. Press
Related Report
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