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Immunological aspects of cancer stem cell and spermatogenesis

Research Project

Project/Area Number 15H04722
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionSapporo Medical University

Principal Investigator

HIROHASHI Yoshihiko  札幌医科大学, 医学部, 准教授 (30516901)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Keywordsがん幹細胞 / 免疫療法 / 班精巣抗原 / がん精巣抗原 / 細胞障害性T細胞 / OR7C1 / BORIS / CTL
Outline of Final Research Achievements

In this research project, we isolated highly tumorigenic cancer stem cells from human colon cancer, cervical cancer and lung cancer cells. We examined the expression profiles of cancer stem cells by cDNA microarry, and we found that numbers of testis-related genes are expressed in cancer stem cells. Those are OR7C1, BORIS SMCP and DNAJB8. Importantly, gene specific knockdown using siRNAs revealed that testis-related genes have important role in the maintenance of cancer stem cells. And we also identified testis-related gene products can be targeted by immunotherapy. These results indicate that immunotherapy targeting testis-related gene products is a promising approach to target cancer stem cells.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • Research Products

    (21 results)

All 2018 2017 2016 2015

All Journal Article (12 results) (of which Peer Reviewed: 12 results,  Open Access: 8 results,  Acknowledgement Compliant: 8 results) Presentation (9 results) (of which Invited: 4 results)

  • [Journal Article] Cellular stress induces cancer stem-like cells through expression of DNAJB8 by activation of heat shock factor 12018

    • Author(s)
      Kusumoto Hiroki、Hirohashi Yoshihiko、Nishizawa Satoshi、Yamashita Masamichi、Yasuda Kazuyo、Murai Aiko、Takaya Akari、Mori Takashi、Kubo Terufumi、Nakatsugawa Munehide、Kanaseki Takayuki、Tsukahara Tomohide、Kondo Toru、Sato Noriyuki、Hara Isao、Torigoe Toshihiko
    • Journal Title

      Cancer Science

      Volume: 109 Issue: 3 Pages: 741-750

    • DOI

      10.1111/cas.13501

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ST6GALNAC1 plays important roles in enhancing cancer stem phenotypes of colorectal cancer via the Akt pathway.2017

    • Author(s)
      Ogawa T, Hirohashi Y, Murai A, Nishidate T, Okita K, Wang L, Ikehara Y, Satoyoshi T, Usui A, Kubo T, Nakastugawa M, Kanaseki T, Tsukahara T, Kutomi G, Furuhata T, Hirata K, Sato N, Mizuguchi T, Takemasa I, Torigoe T.
    • Journal Title

      Oncotarget

      Volume: 8 Issue: 68 Pages: 112550-112564

    • DOI

      10.18632/oncotarget.22545

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of antigenic peptides from novel renal cancer stem-like cell antigen, DNAJB82017

    • Author(s)
      Nishizawa S, Hirohashi Y, Kusumoto H, Wakamiya T, Iguchi T, Yamashita S, Iba A, Kikkawa K, Kohjimoto Y, Torigoe T, Hara I
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 494 Issue: 3-4 Pages: 693-699

    • DOI

      10.1016/j.bbrc.2017.10.146

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comprehensive single-cell transcriptome analysis reveals heterogeneity in endometrioid adenocarcinoma tissues.2017

    • Author(s)
      Hashimoto S, Tabuchi Y, Yurino H, Hirohashi Y, Deshimaru S, Asano T, Mariya T, Oshima K, Takamura Y, Ukita Y, Ametani A, Kondo N, Monma N, Takeda T, Misu S, Okayama T, Ikeo K, Saito T, Kaneko S, Suzuki Y, Hattori M, Matsushima K, et al.
    • Journal Title

      Sci Rep

      Volume: 7 Issue: 1 Pages: 14225-14225

    • DOI

      10.1038/s41598-017-14676-3

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishment and analysis of cancer stem-like and non-cancer stem-like cells from the human colon cancer cell line SW4802016

    • Author(s)
      Akari Takaya, Yoshihiko Hirohashi, et al.
    • Journal Title

      PLOS One

      Volume: 14;11(7) Issue: 7 Pages: e0158903-e0158903

    • DOI

      10.1371/journal.pone.0158903

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Olfactory Receptor Family 7 Subfamily C Member 1 Is a Novel Marker of Colon Cancer-Initiating Cells and Is a Potent Target of Immunotherapy2016

    • Author(s)
      Rena Morita, Yoshihiko Hirohashi, et al.
    • Journal Title

      Clinical Cancer Research

      Volume: 1;22(13) Issue: 13 Pages: 3298-308

    • DOI

      10.1158/1078-0432.ccr-15-1709

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Plasticity of lung cancer stem-like cells is regulated by the transcription factor HOXA5 that is induced by oxidative stress2016

    • Author(s)
      Hiroshi Saijo, Yoshihiko Hirohashi, et al.
    • Journal Title

      Oncotarget

      Volume: 2;7(31) Issue: 31 Pages: 50043-50056

    • DOI

      10.18632/oncotarget.10571

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] MAPK13 is preferentially expressed in gynecological cancer stem cells and has a role in the tumor-initiation.2016

    • Author(s)
      Yasuda K, Hirohashi Y, Kuroda T, Takaya A, Kubo T, Kanaseki T, Tsukahara T, Hasegawa T, Saito T, Sato N, Torigoe T.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 472 Issue: 4 Pages: 643-647

    • DOI

      10.1016/j.bbrc.2016.03.004

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Matrix metalloproteinase-10 regulates stemness of ovarian cancer stem-like cells by activation of canonical Wnt signaling and can be a target of chemotherapy-resistant ovarian cancer.2016

    • Author(s)
      Mariya T, Hirohashi Y, Torigoe T, Tabuchi Y, Asano T, Saijo H, Kuroda T, Yasuda K, Mizuuchi M, Saito T, Sato N
    • Journal Title

      Oncotarget

      Volume: in press Issue: 18 Pages: 1-17

    • DOI

      10.18632/oncotarget.8645

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Olfactory receptor family receptor, family 7, subfamily C, member 1 is a novel marker of colon cancer-initiating cells and is a potent target of immunotherapy.2016

    • Author(s)
      Morita R, Hirohashi Y, Torigoe T, Inoda S, Takahashi A, Mariya T, Asanuma H, Tamura Y, Tsukahara T, Kanaseki T, Kubo T, Kutomi G, Mizuguchi T, Terui T, Ishitani K, Hashino S, Kondo T, Minagawa N, Takahashi N, Taketomi A, Todo S, Asaka M, Sato N.
    • Journal Title

      Clin Cancer Res

      Volume: in press

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Brother of the regulator of the imprinted site (BORIS) variant subfamily 6 is involved in cervical cancer stemness and can be a target of immunotherapy.2016

    • Author(s)
      Asano T, Hirohashi Y, Torigoe T, Mariya T, Horibe R, Kuroda T, Tabuchi Y, Saijo H, Yasuda K, Mizuuchi M, Takahashi A, Asanuma H, Hasegawa T, Saito T, Sato N.
    • Journal Title

      Oncotarget

      Volume: 7 Issue: 10 Pages: 11223-11237

    • DOI

      10.18632/oncotarget.7165

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Immune responses to human cancer stem-like cells/cancer-initiating cells.2016

    • Author(s)
      Hirohashi Y, Torigoe T, Tsukahara T, Kanaseki T, Kochin V, Sato N
    • Journal Title

      Cancer Sci

      Volume: 107 Issue: 1 Pages: 12-17

    • DOI

      10.1111/cas.12830

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] がん幹細胞に注目したがん免疫療法2017

    • Author(s)
      廣橋良彦、塚原智英、金関貴幸、中津川宗秀、久保輝文、鳥越俊彦
    • Organizer
      第76回日本癌学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Immunoreaction to cancer stem cells2017

    • Author(s)
      廣橋良彦
    • Organizer
      第63回日本病理学会秋期特別総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 癌精巣抗原BORISは婦人科癌幹細胞性に関与し、免疫療法のターゲットになりうる2017

    • Author(s)
      廣橋良彦、浅野拓也、鳥越俊彦
    • Organizer
      第56回日本臨床細胞学会秋期大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] がん精巣抗原は、がん幹細胞標的免疫療法の有力な候補になる2016

    • Author(s)
      廣橋 良彦
    • Organizer
      第105回日本病理学会総会
    • Place of Presentation
      仙台国際センター(仙台市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] がん幹細胞標的免疫療法の開発2016

    • Author(s)
      廣橋 良彦
    • Organizer
      第14回日本臨床腫瘍学会学術集会
    • Place of Presentation
      神戸国際展示場・神戸国際会議場(神戸市)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 癌精巣抗原BORISの肺癌幹細胞様細胞における発現とBORIS抗原を標的とした免疫療法の検討2016

    • Author(s)
      堀部 亮多、廣橋 良彦
    • Organizer
      第20回日本がん免疫学会総会
    • Place of Presentation
      大阪国際交流センター(大阪市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] がん幹細胞を標的とした免疫療法2015

    • Author(s)
      廣橋良彦
    • Organizer
      第5回北海道探索病理学研究シンポジウム
    • Place of Presentation
      ニューオータニイン札幌(札幌市)
    • Year and Date
      2015-10-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Establishment of cancer stem cell-targeting immunotherapy2015

    • Author(s)
      廣橋良彦
    • Organizer
      第13回日本臨床腫瘍学会学術集会
    • Place of Presentation
      ロイトン札幌(札幌市)
    • Year and Date
      2015-07-16
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 免疫療法でがんは治せるのか?  ~次世代のがん免疫療法~2015

    • Author(s)
      廣橋良彦
    • Organizer
      日本耳鼻咽喉科学会第98回和歌山地方部会総会
    • Place of Presentation
      和歌山県民文化会館(和歌山市)
    • Year and Date
      2015-07-05
    • Related Report
      2015 Annual Research Report
    • Invited

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Published: 2015-04-16   Modified: 2019-03-29  

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