Mechanism for the formation of cheating organella of Toxoplasma gondii

• Project/Area Number: 15H04726
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: Nagamune Kisaburo 国立感染症研究所, 寄生動物部, 室長 (90314418)
• Co-Investigator(Kenkyū-buntansha): 案浦 健 国立感染症研究所, 寄生動物部, 主任研究官 (90407239)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2015: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: 原虫 / オルガネラ / リクルート / トキソプラズマ

Outline of Final Research Achievements

It is known that Toxoplasma gondii can recruit the host mitochondria to its parasitophorous vacuole. First of this study, I identified 7 candidates responsible for this phenomenon, and evaluate the ability for the recruitment using with overexpressors. As a result, I could identify four genes responsible for the mitochondrial recruitment of T. gondii. I further tried to knock-out these genes. However, I could not knock-out three of them because they might be essential. I established knock-out mutant of remaining gene. This mutant decreased the recruitment ability of the host mitochondria.

Research Products

• [Journal Article] Toxoplasma gondii seroprevalence in goats, cats and humans in Russia (2018)
  • Author(s): Shuralev, E.A., Shamaev, N.D., Mukminov, M.N., Nagamune, K., Taniguchi, Y., Saito, T., Kitoh, K., Arleevskaya, M., Fedotova, A.Y., Abdulmanova, D.R., Aleksandrova, N.M., Efimova, M.A., Yarullin, A.I., Valeeva, A.R., Khaertynov, K.S., Takashima, Y.
  • Journal Title: Parasitol. Int. Volume: 67 Issue: 2 Pages: 112-114
  • DOI: 10.1016/j.parint.2017.10.014
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Toxoplasma gondii RON4 binds to heparan sulfate on the host cell surface (2018)
  • Author(s): Takemae, H., Kobayashi, K., Sugi, T., Han, Y., Gong, H., Ishiwa, A., Recuenco, F. C., Murakoshi, F., Takano, R., Murata, Y., Nagamune, K., Horimoto, T., Akashi, H., Kato, K.
  • Journal Title: Parasitol. Int. Volume: 67 Issue: 2 Pages: 123-130
  • DOI: 10.1016/j.parint.2017.10.008
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Fates of Evolutionarily Distinct, Plastid- type Glyceraldehyde 3-phosphate Dehydrogenase Genes in Kareniacean Dinoflagellates (2018)
  • Author(s): Kamikawa, R., Yazaki, E., Tahara, M., Sakura, T., Matsuo, E., Nagamune, K., Hashimoto, T., Inagaki, Y.
  • Journal Title: J. Eukaryot. Microbiol. Volume: 印刷中 Issue: 5 Pages: 669-678
  • DOI: 10.1111/jeu.12512
  • Peer Reviewed / Open Access
• [Journal Article] Plant hormone cytokinins control cell cycle progression and plastid replication in apicomplexan parasites (2017)
  • Author(s): Andrab, S.B.A., Tahara,M., Matsubara,R., Toyama,T., Aonuma,H., Sakakibara,H., Suematsu,M., Tanabe,K., Nozaki,T., Nagamune, K.
  • Journal Title: Parasitol. Int. Volume: 67 Issue: 1 Pages: 47-58
  • DOI: 10.1016/j.parint.2017.03.003
  • Peer Reviewed / Open Access
• [Journal Article] トキソプラズマが宿主細胞のオルガネラを引き寄せるメカニズム (2017)
  • Author(s): 福本隼平、永宗喜三郎
  • Journal Title: 医学と薬学 Volume: 74 Pages: 1217-1220
• [Journal Article] In vitro and in vivo effects of the phytohormone inhibitor fluridone against Neospora caninum infection (2016)
  • Author(s): bañez, R.H., Leesombun, A., Nishimura, M., Matsubara, R., Kojima, M., Sakakibara, H., Nagamune, K., Nishikawa, Y.
  • Journal Title: Parasitol. Int. Volume: 65 Issue: 4 Pages: 319-322
  • DOI: 10.1016/j.parint.2016.03.009
  • NAID: 120006390951
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A host cell membrane microdomain is a critical factor for organelle discharge by Toxoplasma gondii (2016)
  • Author(s): Tahara, M., Andrabi, S.B., Matsubara, R., Aonuma, H., Nagamune K.
  • Journal Title: Parasitol. Int. Volume: 65 Issue: 5 Pages: 378-388
  • DOI: 10.1016/j.parint.2016.05.012
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Toxoplasma gondii Infection in Mice Impairs Long-Term Fear Memory Consolidation Through Dysfunction of the Cortex and Amygdala (2016)
  • Author(s): Ihara, F., Nishimura, M., Muroi, Y., Mahmoud, M.E., Yokoyama, N., Nagamune, K., Nishikawa, Y.
  • Journal Title: Infect Immun. Volume: 84 Issue: 10 Pages: 2861-2871
  • DOI: 10.1128/iai.00217-16
  • NAID: 120006390959
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Investigation into the Physiological Significance of the Phytohormone Abscisic Acid in Perkinsus marinus, an Oyster Parasite Harboring a Non-Photosynthetic Plastid (2016)
  • Author(s): Sakamoto, H, Suzuki, S., Nagamune, K., Kita, K., Matsuzaki, M.
  • Journal Title: J. Eukaryot. Microbiol. Volume: 印刷中 Issue: 4 Pages: 440-446
  • DOI: 10.1111/jeu.12379
  • Peer Reviewed / Open Access
• [Journal Article] Increased CD11b+ Gr-1+ cell population in the placenta after infection with Toxoplasma gondii. (2015)
  • Author(s): Takeshima K, Sato K, Nabekura T, Nagamune K, Hamada H, Yoshikawa H, Shibuya A, Shibuya K.
  • Journal Title: Microbiol. immunol Volume: 59 Issue: 2 Pages: 95-98
  • DOI: 10.1111/1348-0421.12225
  • NAID: 120007130301
  • Peer Reviewed / Open Access
• [Journal Article] Prevalence and dynamics of antibodies against Toxoplasma gondii in kids born from naturally infected goats (2015)
  • Author(s): Rahman, M., Alauddin, M., Hossain, K.M., Islam, M.H., Kitoh, K., Nagamune, K., Takashima, Y.
  • Journal Title: Parasitol. Int. Volume: 64 Issue: 5 Pages: 389-391
  • DOI: 10.1016/j.parint.2015.05.015
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evaluation of aegerolysins as novel tools to detect and visualize ceramide phosphoethanolamine, a major sphingolipid in invertebrates (2015)
  • Author(s): Bhat HB, Ishitsuka R, Inaba T, Murate M, Abe M, Makino A, Kohyama-Koganeya A, Nagao K, Kurahashi A, Kishimoto T, Tahara M, Yamano A, Nagamune K, Hirabayashi Y, Juni N, Umeda M, Fujimori F, Nishibori K, Yamaji-Hasegawa A, Greimel P, Kobayashi T.
  • Journal Title: FASEB J Volume: 29(9) Issue: 9 Pages: 3920-34
  • DOI: 10.1096/fj.15-272112
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Plant Hormone Salicylic Acid Produced by a Malaria Parasite Controls Host Immunity and Cerebral Malaria Outcome. (2015)
  • Author(s): Matsubara R, Aonuma H, Kojima M, Tahara M, Andrabi SB, Sakakibara H, Nagamune K.
  • Journal Title: PLoS One Volume: 10 Issue: 10 Pages: e0140559-e0140559
  • DOI: 10.1371/journal.pone.0140559
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] トキソプラズマにおける宿主ミトコンドリアリクルート機構の解明 (2018)
  • Author(s): 福本隼平、佐倉孝哉、松原立真、田原美智留、松崎素道、永宗喜三郎
  • Organizer: 第87 回日本寄生虫学会大会
• [Presentation] トキソプラズマ日本分離株のゲノムワイドSNP解析 (2018)
  • Author(s): 松崎素道、福本隼平、喜屋武向子、正谷達謄、松尾智英、村上麻美、高島康弘、西川義文、永宗喜三郎
  • Organizer: 第87 回日本寄生虫学会大会
• [Presentation] 肝内型マラリア原虫における核増殖メカニズムの解明 (2018)
  • Author(s): 荒木球沙、川合覚、小林宏尚、片岡紀代、角田宗一郎、永宗喜三郎、野崎智義、久枝一、案浦健
  • Organizer: 第87 回日本寄生虫学会大会
• [Presentation] ナショナルデータベース（NDB Japan）を用いた日本国内の寄生虫症発生動向の解析 (2018)
  • Author(s): 森嶋康之、永宗喜三郎、杉山 広、山﨑 浩
  • Organizer: 第87 回日本寄生虫学会大会
• [Presentation] レセプト情報解析から見えてきた日本におけるトキソプラズマ症の実態 (2018)
  • Author(s): 永宗喜三郎、森嶋康之
  • Organizer: 第87 回日本寄生虫学会大会
• [Presentation] 日本におけるトキソプラズマのタイピングと病原性 (2017)
  • Author(s): 福本隼平、山野安規徳、松原立真、竹内史比古、喜屋武向子、正谷達謄、松尾智英、村上麻美、高島康弘、永宗喜三郎
  • Organizer: 第86 回日本寄生虫学会大会
• [Presentation] 熱帯熱マラリア原虫の赤内期における新しい輸送経路の解析 (2017)
  • Author(s): 北園和泉、平井智浩、永宗喜三郎、野崎智義、中野由美子
  • Organizer: 第25 回分子寄生虫学ワークショップ・第14回分子寄生虫・マラリア研究フォーラム合同大会
• [Presentation] 肝内型マラリア原虫における核増殖メカニズムの解明 (2017)
  • Author(s): 荒木球沙、川合覚、小林宏尚、片岡紀代、永宗喜三郎、野崎智義、案浦健
  • Organizer: 第50回日本原生生物学会、第1回日本共生生物学会合同大会
• [Presentation] トキソプラズマにおける宿主ミトコンドリアリクルート機構の解明 (2017)
  • Author(s): 福本隼平、佐倉孝哉、松原立真、田原美智留、永宗喜三郎
  • Organizer: 第50回日本原生生物学会、第1回日本共生生物学会合同大会
  • Invited
• [Presentation] The molecular genotyping and dissection of Japanese Toxoplasma gondii reveals an unidentified strategy for successful survival of the parasite (2017)
  • Author(s): Nagamune, K., Fukumoto, J., Yamano, A., Tahara, M., Matsubara, R.
  • Organizer: 14th The Toxoplasma gondii research community biennial meeting
  • Int'l Joint Research
• [Presentation] Elucidating the mechanism of host mitochondrial recruitment of Toxoplasma gondii (2016)
  • Author(s): Fukumoto, J., Sakura, T., Matsubara, R., Nagamune, K.
  • Organizer: The 13th International Colloquium on Endocytobiology and Symbiosis
  • Place of Presentation: 京都市
  • Year and Date: 2016-09-10
  • Int'l Joint Research
• [Presentation] トキソプラズマにおける宿主オルガネラリクルート機構の解析にむけて (2016)
  • Author(s): 福本隼平、佐倉孝哉、永宗喜三郎
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市
  • Year and Date: 2016-03-18
• [Presentation] トキソプラズマにおける宿主オルガネラリクルート機構の解析にむけて (2015)
  • Author(s): 福本隼平、佐倉孝哉、松原立真、田原美智留、永宗喜三郎
  • Organizer: 第48回日本原生生物学会大会
  • Place of Presentation: 東京都
  • Year and Date: 2015-11-06
• [Presentation] Investigation for the Understanding of the Mechanism of Host Organelle Recruitment by Toxoplasma gondii (2015)
  • Author(s): Fukumoto, J., Sakura, T., Matsubara, R., Nagamune, K.
  • Organizer: 2nd International Symposium and 4th Annual Research Meeting on Matryoshka-type Evolution of Eukaryotic Cells
  • Place of Presentation: つくば市
  • Year and Date: 2015-09-30
  • Int'l Joint Research
• [Presentation] トキソプラズマにおける宿主オルガネラリクルート機構の解析にむけて (2015)
  • Author(s): 福本隼平、佐倉孝哉、松原立真、田原美智留、永宗喜三郎
  • Organizer: 第23 回分子寄生虫学ワークショップ・第13回分子寄生虫・マラリア研究フォーラム合同大会
  • Place of Presentation: 帯広市
  • Year and Date: 2015-08-30
• [Book] 小児臨床検査のポイント2017 (2017)
  • Author(s): 永宗喜三郎
  • Total Pages: 4
  • Publisher: 東京医学社
• [Book] 小児内科 (2017)
  • Author(s): 永宗喜三郎
  • Publisher: 東京医学社
• [Book] 化学療法の領域 (2016)
  • Author(s): 松原立真、永宗喜三郎
  • Total Pages: 10
  • Publisher: 医薬ジャーナル社
• [Book] 遺伝 (2016)
  • Author(s): 松原立真、永宗喜三郎
  • Total Pages: 6
  • Publisher: エヌ・ティー・エス
• [Book] 衛生の友 (2016)
  • Author(s): 永宗喜三郎
  • Total Pages: 1
  • Publisher: サラヤ