High-throughput screening for chemical compounds which restict intracellular bacteria

• Project/Area Number: 15H04728
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Gifu University (2016-2017); Osaka University (2015)
• Principal Investigator: Nagai Hiroki 岐阜大学, 大学院医学系研究科, 教授 (80222173)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2015: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
• Keywords: 細菌 / 化合物ライブラリ / 細胞内寄生 / 化合物ライブラリー

Outline of Final Research Achievements

Legionella pneumophila is a causative bacterium of a severe form of pneumonia known as legionellosis. We conducted high-throughput screening for chemical compounds which specifically impair Legionella growth within host cells. Some of the compounds we have identified appear to target host cellular pathways which have never been implicated in intracellular survival and replication of microorganisms.

Research Products

• [Journal Article] LotA, a Legionella deubiquitinase, has dual catalytic activity and contributes to intracellular growth. (2018)
  • Author(s): Kubori T, Kitao T, Ando H and Nagai H.
  • Journal Title: Cell Microbiol. Volume: Mar 15 Issue: 7 Pages: e12840-e12840
  • DOI: 10.1111/cmi.12840
  • Peer Reviewed
• [Journal Article] Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila for Negative Stain Electron Microscopy Studies (2017)
  • Author(s): Kubori Tomoko、Nagai Hiroki
  • Journal Title: Bio-protocol Volume: 7 Issue: 8
  • DOI: 10.21769/bioprotoc.2229
  • Peer Reviewed / Open Access
• [Journal Article] Legionella RavZ Plays a Role in Preventing Ubiquitin Recruitment to Bacteria-Containing Vacuoles. (2017)
  • Author(s): Kubori T, Bui XT, Hubber A and Nagai H.
  • Journal Title: Front Cell Infect Microbiol. Volume: 7 Pages: 384-384
  • DOI: 10.3389/fcimb.2017.00384
  • Peer Reviewed / Open Access
• [Journal Article] Bacterial secretion system skews the fate of Legionella-containing vacuoles towards LC3-associated phagocytosis. (2017)
  • Author(s): Hubber A., Kubori T., Coban C., Matsuzawa T., Ogawa M., Kawabata T., Yoshimori T., and Nagai H.
  • Journal Title: Sci Rep. Volume: 7 Issue: 1 Pages: 44795-44795
  • DOI: 10.1038/srep44795
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The type IVB secretion system: an enigmatic chimera. (2016)
  • Author(s): Kubori T and *Nagai H.
  • Journal Title: Curr Opin Microbiol. Volume: 29 Pages: 22-9
  • DOI: 10.1016/j.mib.2015.10.001
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] T4BSS: a pivotal tool to establish intracellular lifestyle of bacteria (2017)
  • Author(s): Hiroki Nagai
  • Organizer: 5th International Symposium of the Collaborative Research Centre 766 (SFB766): The Bacterial Cell Envelope
  • Int'l Joint Research / Invited
• [Presentation] T4BSS: a pivotal tool to establish intracellular lifestyle of bacteria (2016)
  • Author(s): Hiroki Nagai
  • Organizer: T4SS 2016
  • Place of Presentation: Schloss Hirschberg, Germany
  • Year and Date: 2016-12-08
  • Int'l Joint Research / Invited
• [Presentation] レジオネラの分泌装置とエフェクターから紐解く感染細胞内での卓越した宿主細胞支配機構 (2016)
  • Author(s): 永井宏樹
  • Organizer: 第31回日本微生物生態学会大会
  • Place of Presentation: 日本大学藤沢キャンパス
  • Year and Date: 2016-10-25
  • Invited
• [Presentation] Type IVB Secretion System as an Essential Tool for Intracellular Life (2016)
  • Author(s): Hiroki Nagai and Tomoko Kubori
  • Organizer: 第13回韓日国際微生物学シンポジウム
  • Place of Presentation: Gyeongju, Korea
  • Year and Date: 2016-05-13
  • Int'l Joint Research / Invited
• [Remarks] 研究室webページ
  • URL: https://sites.google.com/site/nagailabjp/
• [Remarks] 研究室webページ
  • URL: http://nagailab.biken.osaka-u.ac.jp/