Development of diagnostic method for atherosclerosis targeting adipokine binding proteins
| Project/Area Number |
15H04762
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Osaka University |
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Principal Investigator |
Kihara Shinji 大阪大学, 医学系研究科, 教授 (20332736)
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| Co-Investigator(Kenkyū-buntansha) |
山本 浩靖 大阪大学, 医学系研究科, 准教授 (00631201)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2015: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Keywords | 蛋白質 / 医療・福祉 / 分析科学 / 細胞・組織 / 生体分子 / 分析学 / 医療･福祉 / 分析科 / 細胞･組織 |
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| Outline of Final Research Achievements |
Based on the combination of immunoprecipitation and mass spectrometry analysis of human serum and cultured human cells, we identified E-selectin ligand (ESL)-1and Mac2 Binding Protein (M2BP) as novel adiponectin binding proteins.
We investigated that adiponectin has anti-atherosclerotic function through the inhibition of monocyte adhesion on endothelium via direct association between adiponectin and ESL-1 on monocyte. In addition, we found that serum adiponectin-M2BP complex levels were markedly higher in patients with coronary artery disease (CAD) than in healthy subjects, and M2BP abrogated the anti-atherogenic effects of adiponectin on cultured endothelial cells. Moreover, we elucidated that serum adiponectin-cystatin C complex levels associated with coronary plaque vulnerability evaluated by intravascular ultrasound in CAD patients.
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Report
(4 results)
Research Products
(10 results)
