Development of a novel therapy and assessment method for NASH targeting glycosylation modification

• Project/Area Number: 15H04810
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Osaka University
• Principal Investigator: Kamada Yoshihiro 大阪大学, 医学系研究科, 准教授 (30622609)
• Co-Investigator(Kenkyū-buntansha): 真鍋 良幸 大阪大学, 理学研究科, 助教 (00632093) ; 三善 英知 大阪大学, 医学系研究科, 教授 (20322183)
• Research Collaborator: Ono Masafumi ; Sumida Yoshio ; Hyogo Hideyuki
• Project Period (FY): 2015-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2016: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
• Keywords: NAFLD/NASH / バイオマーカー / フコシル化ハプトグロビン / Mac-2 binding protein / Fut8 / NASH/NAFLD / NASH / Mac-2bp / NAFLD / 肝臓学 / 糖鎖 / fucosylation

Outline of Final Research Achievements

We found that liver fucosylation increased during the progression of nonalcoholic fatty liver disease (NAFLD). Using biopsy-proven 510 NAFLD patients and 2122 health checkpup subjects, we developed two glyco-biomarkers, fucosylated haptoglobin (Fuc-Hpt) and Mac-2 binding protein (Mac-2bp). These glyco-biomarkers were superior than other conventional biomarkers, and well-diagnosed NAFLD disease severity. We found that Fuc-Hpt is a useful for the prediction of ballooning hepatocyte presence, and Mac-2bp is useful for the diagnosis of liver fibrosis progression in NAFLD patients. Combination of these biomarkers can be used as a superior NAFLD biomarker.
Among fucosylation, core-fucosylation specially increased in NAFLD liver. We previously found core-fucose specific lectin, PhoSL, from Japanese mushroom. PhoSL administration improved NAFLD development in mouse NAFLD model. This result suggested the utility of PhoSL lectin as a “glyco-therapy” for NAFLD.

Academic Significance and Societal Importance of the Research Achievements

NAFLD診断はこれまで確立した方法が肝生検による組織診断しかなかった。治療効果判定のために頻回に肝生検を行うことは困難であるため、非侵襲的に病態進展を評価する方法の開発が喫緊の課題となっている。本研究によって糖鎖バイオマーカーにより、血液を用いてNAFLD病態進展を評価することが可能となった。またマウスを用いた基礎的研究によってPhoSLというキノコから抽出したタンパク質がNAFLD病態進展を抑制することがわかった。PhoSLはコアフコースという糖鎖に結合するタンパク質である。本研究の成果は糖鎖を標的とした新しいヒトNAFLD治療法の開発につながるものと期待される。

Research Products

• [Journal Article] Impact of fatty pancreas and lifestyle on the development of subclinical chronic pancreatitis in healthy people undergoing a medical checkup (2019)
  • Author(s): Fujii Makoto、Ohno Yuko、Yamada Makoto、Kamada Yoshihiro、Miyoshi Eiji
  • Journal Title: Environmental Health and Preventive Medicine Volume: 24 Issue: 1 Pages: 10-10
  • DOI: 10.1186/s12199-019-0763-2
  • NAID: 210000130326
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Clinical features of hepatocellular carcinoma in nonalcoholic fatty liver disease patients without advanced fibrosis (2019)
  • Author(s): Kodama Kazuhisa、Kawaguchi Takumi、Hyogo Hideyuki、Nakajima Tomoaki、Ono Masafumi、Sumida Yoshio、Kamada Yoshihiro、Fujii Hideki、Seko Yuya、Takehara Tetsuo、Itoh Yoshito、Nakajima Atsushi、Masaki Naohiko、Torimura Takuji、Saibara Toshiji、Karino Yoshiyasu、Chayama Kazuaki、Tokushige Katsutoshi
  • Journal Title: Journal of Gastroenterology and Hepatology Volume: in press Issue: 9 Pages: 1626-1632
  • DOI: 10.1111/jgh.14608
  • Peer Reviewed / Open Access
• [Journal Article] Core fucose is essential glycosylation for CD14-dependent Toll-like receptor 4 and Toll-like receptor 2 signaling in macrophages (2018)
  • Author(s): Nakayama K, Wakamatsu K, Fujii H, Shinzaki S, Takamatsu S, Kitazume S, Kamada Y, Takehara T, Taniguchi N, Miyoshi E
  • Journal Title: J Biochem Volume: ー Issue: 3 Pages: 227-237
  • DOI: 10.1093/jb/mvy098
  • NAID: 40021845862
  • Peer Reviewed
• [Journal Article] Possible involvement of Enterococcus infection in the pathogenesis of chronic pancreatitis and cancer (2018)
  • Author(s): Maekawa Tomohiro、Fukaya Risako、Takamatsu Shinji、Itoyama Saki、Fukuoka Tomoya、Yamada Makoto、Hata Tomoki、Nagaoka Satoshi、Kawamoto Koichi、Eguchi Hidetoshi、Murata Kohei、Kumada Takashi、Ito Toshifumi、Tanemura Masahiro、Fujimoto Kahoko、Tomita Yasuhiko、Tobe Toru、Kamada Yoshihiro、Miyoshi Eiji
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 506 Issue: 4 Pages: 962-969
  • DOI: 10.1016/j.bbrc.2018.10.169
  • Peer Reviewed / Open Access
• [Journal Article] Haptoglobin phenotype is a critical factor in the use of fucosylated haptoglobin for pancreatic cancer diagnosis (2018)
  • Author(s): Morishita Koichi、Ito Nami、Koda Sayaka、Maeda Megumi、Nakayama Kotarosumitomo、Yoshida Kiyoshi、Takamatsu Shinji、Yamada Makoto、Eguchi Hidetoshi、Kamada Yoshihiro、Miyoshi Eiji
  • Journal Title: Clinica Chimica Acta Volume: 487 Pages: 84-89
  • DOI: 10.1016/j.cca.2018.09.001
  • Peer Reviewed
• [Journal Article] A Data Mining-based Prognostic Algorithm for NAFLD-related Hepatoma Patients: A Nationwide Study by the Japan Study Group of NAFLD (2018)
  • Author(s): Kawaguchi T, Tokushige K, Hyogo H, Aikata H, Nakajima T, Ono M, Kawanaka M, Sawada K, Imajo K, Kamada Y, Saibara T, Karino Y, Chayama K, Hashimoto E, George J, Torimura T
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 10434-10434
  • DOI: 10.1038/s41598-018-28650-0
  • Peer Reviewed
• [Journal Article] Targeting the mevalonate pathway is a novel therapeutic approach to inhibit oncogenic FoxM1 transcription factor in human hepatocellular carcinoma (2018)
  • Author(s): Ogura S, Yoshida Y, Kurahashi T, Egawa M, Furuta K, Kiso S, Kamada Y, Hikita H, Eguchi H, Ogita H, Doki Y, Mori M, Tatsumi T, Takehara T.
  • Journal Title: Oncotarget Volume: 9 Issue: 30 Pages: 21022-21035
  • DOI: 10.18632/oncotarget.24781
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of an antibody specific for cancer-associated haptoglobin: A possible implication of clinical investigation. (2018)
  • Author(s): Nishino K, Koda S, Kataoka N, Takamatsu S, Nakano M, Ikeda S, Kamamatsu Y, Morishita K, Moriwaki K, Eguchi H, Yamamoto E, Kikkawa F, Tomita Y, Kamada Y, Miyoshi E.
  • Journal Title: Oncotarget Volume: 9 Issue: 16 Pages: 12732-12744
  • DOI: 10.18632/oncotarget.24332
  • NAID: 120006799360
  • Peer Reviewed / Open Access
• [Journal Article] Targeting the mevalonate pathway is a novel therapeutic approach to inhibit oncogenic FoxM1 transcription factor in human hepatocellular carcinoma. (2018)
  • Author(s): Ogura S, Yoshida Y, Kurahashi T, Egawa M, Furuta K, Kiso S, Kamada Y, Hikita H, Eguchi H, Ogita H, Doki Y, Mori M, Tatsumi T, Takehara T.
  • Journal Title: Oncotarget Volume: in press
  • Peer Reviewed / Open Access
• [Journal Article] 肥満の及ぼす人体への影響～特にアディポサイトカイン～ (2018)
  • Author(s): 鎌田佳宏、三善英知、竹原徹郎
  • Journal Title: 消化器・肝臓内科 Volume: 3 Pages: 153-160
• [Journal Article] Fatty Acid-Mediated Stromal Reprogramming of Pancreatic Stellate Cells Induces Inflammation and Fibrosis That Fuels Pancreatic Cancer. (2017)
  • Author(s): Hata T, Kawamoto K, Eguchi H, Kamada Y, Takamatsu S, Maekawa T, Nagaoka S, Yamada D, Iwagami Y, Asaoka T, Noda T, Wada H, Gotoh K, Masamune A, Miyoshi E, Mori M, Doki Y.
  • Journal Title: Pancreas Volume: 46 Issue: 10 Pages: 1259-1266
  • DOI: 10.1097/mpa.0000000000000943
  • Peer Reviewed / Open Access
• [Journal Article] Mac-2 Binding Protein is a Useful Liver Fibrosis Biomarker for NAFLD/NASH (2017)
  • Author(s): Kamada Y and Miyoshi E.
  • Journal Title: Trends in Glycoscience and Glycotechnology Volume: 29 Issue: 170 Pages: E85-E92
  • DOI: 10.4052/tigg.1714.1E
  • NAID: 130006219286
  • ISSN: 0915-7352, 1883-2113
  • Peer Reviewed
• [Journal Article] Use of Mac-2 binding protein as a biomarker for nonalcoholic fatty liver disease diagnosis. (2017)
  • Author(s): Kamada Y, Ono M, Hyogo H, Fujii H, Sumida Y, Yamada M, Mori K, Tanaka S, Maekawa T, Ebisutani Y, Yamamoto A, Takamatsu S, Yoneda M, Kawada N, Chayama K, Saibara T, Takehara T, Miyoshi E.; Japan Study Group of NAFLD (JSG-NAFLD)
  • Journal Title: Hepatol Commun. Volume: 1 Issue: 8 Pages: 780-791
  • DOI: 10.1002/hep4.1080
  • Peer Reviewed / Open Access
• [Journal Article] Reevaluation of Pholiota squarrosa lectin-reactive haptoglobin as a pancreatic cancer biomarker using an improved ELISA system. (2017)
  • Author(s): Kusama K, Okamoto Y, Saito K, Kasahara T, Murata T, Ueno Y, Kobayashi Y, Kamada Y, Miyoshi E.
  • Journal Title: Glycoconj J. Volume: 34 Issue: 4 Pages: 537-544
  • DOI: 10.1007/s10719-017-9772-9
  • Peer Reviewed / Open Access
• [Journal Article] Serum WFA+ -M2BP levels predict liver fibrosis, development of hepatocellular carcinoma, and overall survival: A meta-analysis. (2017)
  • Author(s): Ito K, Murotani K, Nakade Y, Inoue T, Nakao H, Sumida Y, Kamada Y, Yoneda M.
  • Journal Title: J Gastroenterol Hepatol. Volume: 32 Issue: 12 Pages: 1922-1930
  • DOI: 10.1111/jgh.13802
  • Peer Reviewed
• [Journal Article] A novel pathogenesis of inflammatory bowel disease from the perspective of glyco-immunology. (2017)
  • Author(s): Shinzaki S, Iijima H, Fujii H, Kamada Y, Naka T, Takehara T, Miyoshi E.
  • Journal Title: J Biochem. Volume: 印刷中 Issue: 5 Pages: 409-415
  • DOI: 10.1093/jb/mvx010
  • Peer Reviewed
• [Journal Article] Development of alpha1,6-fucosyltransferase inhibitors through the diversity-oriented syntheses of GDP-fucose mimics using the coupling between alkyne and sulfonyl azide. (2017)
  • Author(s): Manabe, Y., Kasahara, S., Takakura, Y., Yang, X., Takamatsu, S., Kamada Y., Miyoshi, M., Yoshidome, D., Fukasea, K.
  • Journal Title: Bioorg. Med. Chem. Volume: in press Issue: 11 Pages: 2844-2850
  • DOI: 10.1016/j.bmc.2017.02.036
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Increased expression of Forkhead box M1 transcription factor is associated with clinicopathological features and confers a poor prognosis in human hepatocellular carcinoma. (2017)
  • Author(s): Egawa M, Yoshida Y, Ogura S, Kurahashi T, Kizu T, Furuta K, Kamada Y, Chatani N, Hamano M, Kiso S, Hikita H, Tatsumi T, Eguchi H, Nagano H, Doki Y, Mori M, Takehara T
  • Journal Title: Hepatol Res. Volume: 印刷中 Issue: 11 Pages: 1196-1205
  • DOI: 10.1111/hepr.12854
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] メタボがんの高危険群囲い込みのための血液バイオマーカーによる非侵襲的診断方法開発 (2017)
  • Author(s): 鎌田佳宏、三善英知
  • Journal Title: 臨床病理 Volume: 7 Pages: 778-785
  • Peer Reviewed
• [Journal Article] 肝疾患の糖鎖バイオマーカー (2017)
  • Author(s): 鎌田佳宏、三善英知
  • Journal Title: BIO Clinica Volume: 4 Pages: 33-37
• [Journal Article] Establishment of mouse Mac-2 binding protein enzyme-linked immunosorbent assay and its application for mouse chronic liver disease models. (2016)
  • Author(s): Iwata A, Kamada Y, Ebisutani Y, Yamamoto A, Ueda Y, Arai H, Fujii H, Takamatsu S, Maruyama N, Maeda M, Takehara T, Miyoshi E.
  • Journal Title: Hepatology Research Volume: 47 Issue: 9 Pages: 1-11
  • DOI: 10.1111/hepr.12819
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hepatic aberrant glycosylation by N-acetylglucosaminyltransferase V accelerates HDL assembly. (2016)
  • Author(s): Kamada Y, Kida S, Hirano KI, Yamaguchi S, Suzuki A, Hashimoto C, Kimura A, Sato M, Fujii H, Sobajima T, Yamamoto A, Ebisutani Y, Takamatsu S, Shinzaki S, Yoshida Y, Yamada M, Nagasaka H, Takehara T, Miyoshi E.
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: 311 Issue: 5 Pages: G859-G868
  • DOI: 10.1152/ajpgi.00231.2016
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Application of glyco-science to the early detection of pancreatic cancer. (2016)
  • Author(s): Miyoshi E, Kamada Y
  • Journal Title: Cancer Sci Volume: 107 Issue: 10 Pages: 1357-1362
  • DOI: 10.1111/cas.13011
  • Peer Reviewed / Open Access
• [Journal Article] Core-fucosylation plays a pivotal role in hepatitis B pseudo virus infection: a possible implication for HBV glycotherapy. (2016)
  • Author(s): Takamatsu S, Shimomura M, Kamada Y, Maeda H, Sobajima T, Hikita H, Iijima M, Okamoto Y, Misaki R, Fujiyama K, Nagamori S, Kanai Y, Takehara T, Ueda K, Kuroda S, Miyoshi E.
  • Journal Title: Glycobiology. Volume: 26 Pages: 1180-1189
  • DOI: 10.1093/glycob/cww067
  • Peer Reviewed
• [Journal Article] Serum Mac-2 binding protein is a novel biomarker for chronic pancreatitis. (2016)
  • Author(s): Maekawa T*, Kamada Y* (equally contributed), Ebisutani Y, Ueda M, Hata T, Kawamoto K, Takamatsu S, Mizutani K, Shimomura M, Sobajima T, Fujii H, Nakayama K, Nishino K, Yamada M, Kumada T, Ito T, Eguchi H, Nagano H, Miyoshi E.
  • Journal Title: World J Gastroenterol Volume: 22 Issue: 17 Pages: 4403-10
  • DOI: 10.3748/wjg.v22.i17.4403
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A glycoproteomic approach to identify novel glycomarkers for cancer stem cells. (2016)
  • Author(s): Sawanobori A, Moriwaki K, Takamatsu S, Kamada Y, Miyoshi E.
  • Journal Title: Proteomics Volume: 16 Issue: 24 Pages: 3073-3080
  • DOI: 10.1002/pmic.201500472
  • Peer Reviewed
• [Journal Article] Site-specific and linkage analyses of fucosylated N-glycans on haptoglobin in sera of patients with various types of cancer: possible implication for the differential diagnosis of cancer. (2016)
  • Author(s): Takahashi S, Sugiyama T, Shimomura M, Kamada Y, Fujita K, Nonomura N, Miyoshi E, Nakano M.
  • Journal Title: Glycoconj J Volume: 33 Issue: 3 Pages: 471-82
  • DOI: 10.1007/s10719-016-9653-7
  • Peer Reviewed / Open Access
• [Journal Article] Specific increase in serum core-fucosylated haptoglobin in patients with chronic pancreatitis. (2016)
  • Author(s): Ueda M, Kamada Y, Takamatsu S, Shimomura M, Maekawa T, Sobajima T, Fujii H, Nakayama K, Nishino K, Yamada M, Kobayashi Y, Kumada T, Ito T, Eguchi H, Nagano H, Miyoshi E.
  • Journal Title: Pancreatology Volume: 16 Issue: 2 Pages: 238-243
  • DOI: 10.1016/j.pan.2016.01.004
  • Peer Reviewed
• [Journal Article] Gab1 adaptor protein acts as a gatekeeper to balance hepatocyte death and proliferation during acetaminophen-induced liver injury in mice. (2016)
  • Author(s): Furuta K, Yoshida Y, Ogura S, Kurahashi T, Kizu T, Maeda S, Egawa M, Chatani N, Nishida K, Nakaoka Y, Kiso S, Kamada Y, Takehara T.
  • Journal Title: Hepatology Volume: 63 Issue: 4 Pages: 1340-1355
  • DOI: 10.1002/hep.28410
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Elevation of CA19-9-Related Novel Marker, Core 1 Sialyl Lewis A, in Sera of Adenocarcinoma Patients Verified by a SRM-Based Method. (2016)
  • Author(s): Tanaka-Okamoto M, Yabu M, Mukai M, Takahashi H, Fujiwara Y, Ohue M, Kamada Y, Miyoshi E, Miyamoto Y.
  • Journal Title: Journal of Proteome Research Volume: 15 Issue: 1 Pages: 152-165
  • DOI: 10.1021/acs.jproteome.5b00893
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Hepatocellular carcinoma in Japanese patients with nonalcoholic fatty liver disease and alcoholic liver disease: multicenter survey. (2016)
  • Author(s): Tokushige K, Hyogo H, Nakajima T, Ono M, Kawaguchi T, Honda K, Eguchi Y, Nozaki Y, Kawanaka M, Tanaka S, Imajo K, Sumida Y, Kamada Y, Fujii H, Suzuki Y, Kogiso T, Karino Y, Munekage K, Kuromatsu R, Oeda S, Yanase M, Mori K, Ogawa Y, Seko Y, Takehara T, Itoh Y, Nakajima A, Kanemasa K, Saibara T
  • Journal Title: J Gastroenterol Volume: 印刷中 Issue: 6 Pages: 586-96
  • DOI: 10.1007/s00535-015-1129-1
  • Peer Reviewed / Open Access
• [Journal Article] Role of aberrant IgG glycosylation in the pathogenesis of inflammatory bowel disease. (2016)
  • Author(s): Miyoshi E, Shinzaki S, Fujii H, Iijima H, Kamada Y, Takehara T.
  • Journal Title: Proteomics Clinical Applications Volume: 未定 Issue: 4 Pages: 384-390
  • DOI: 10.1002/prca.201500089
  • Peer Reviewed
• [Journal Article] N-Acetylglucosaminyltransferase V exacerbates murine colitis with macrophage dysfunction and enhances colitic tumorigenesis. (2016)
  • Author(s): Shinzaki S, Ishii M, Fujii H, Iijima H, Wakamatsu K, Kawai S, Shiraishi E, Hiyama S, Inoue T, Hayashi Y, Kuwahara R, Takamatsu S, Kamada Y, Morii E, Tsujii M, Takehara T, Miyoshi E.
  • Journal Title: J Gastroenterol. Volume: 51 Issue: 4 Pages: 357-369
  • DOI: 10.1007/s00535-015-1119-3
  • Peer Reviewed
• [Journal Article] Ectopic expression of N-acetylglucosaminyltransferase V accelerates hepatic triglyceride synthesis. (2016)
  • Author(s): Kamada Y, Ebisutani Y, Kida S, Mizutani K, Akita M, Yamamoto A, Fujii H, Sobajima T, Terao N, Takamatsu S, Yoshida Y, Takehara T, Miyoshi E
  • Journal Title: Hepatol Res. Volume: 46 Issue: 3 Pages: 1189-129
  • DOI: 10.1111/hepr.12541
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Impact of plasma transaminase levels on the peripheral blood glutamate levels and memory functions in healthy subjects. (2015)
  • Author(s): Kamada Y, Hashimoto R, Yamamori H, Yasuda Y, Takehara T, Fujita Y, Hashimoto K, Miyoshi E.
  • Journal Title: BBA Clin Volume: 5 Pages: 101-107
  • DOI: 10.1016/j.bbacli.2016.02.004
  • Peer Reviewed
• [Journal Article] A novel noninvasive diagnostic method for nonalcoholic steatohepatitis using two glycobiomarkers. (2015)
  • Author(s): Kamada Y, Ono M, Hyogo H, Fujii H, Sumida Y, Mori K, Tanaka S, Yamada M, Akita M, Mizutani K, Fujii H, Yamamoto A, Takamatsu S, Yoshida Y, Itoh Y, Kawada N, Chayama K, Saibara T, Takehara T, Miyoshi E
  • Journal Title: Hepatology. Volume: 62 Issue: 5 Pages: 1433-1443
  • DOI: 10.1002/hep.28002
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Establishment of a novel lectin-antibody ELISA system to determine core-fucosylated haptoglobin. (2015)
  • Author(s): Shimomura M, Nakayama K, Azuma K, Terao N, Nishino K, Takamatsu S, Nakano M, Takahashi S, Kobayashi Y, Murata K, Kamada Y, Miyoshi E.
  • Journal Title: Clin Chim Acta Volume: in press Pages: 30-6
  • DOI: 10.1016/j.cca.2015.03.037
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Value of Fetuin-A as a Predictor of Liver Fibrosis in Patients With Nonalcoholic Fatty Liver Disease. Author's reply. (2015)
  • Author(s): Kamada Y, Miyoshi E.
  • Journal Title: Liver Int Volume: in press Issue: 8 Pages: 2062-2062
  • DOI: 10.1111/liv.12833
  • Peer Reviewed
• [Journal Article] Loss of α1,6-fucosyltransferase suppressed liver regeneration: implication of core fucose in the regulation of growth factor receptor-mediated cellular signaling. (2015)
  • Author(s): Wang Y, Fukuda T, Isaji T, Lu J, Gu W, Lee HH, Ohkubo Y, Kamada Y, Taniguchi N, Miyoshi E, Gu J.
  • Journal Title: Sci Rep Volume: in press Issue: 1 Pages: 925-35
  • DOI: 10.1038/srep08264
  • Peer Reviewed / Open Access
• [Journal Article] Loss of Gab1 adaptor protein in hepatocytes aggravates experimental liver fibrosis in mice. (2015)
  • Author(s): Kizu T, Yoshida Y, Furuta K, Ogura S, Egawa M, Chatani N, Hamano M, Takemura T, Ezaki H, Kamada Y, Nishida K, Nakaoka Y, Kiso S, Takehara T.
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: 308 Issue: 7 Pages: 613-624
  • DOI: 10.1152/ajpgi.00289.2014
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Fucosylation is common type of glycosylation in pancreatic cancer stem cell-like phenotypes. (2015)
  • Author(s): Terao N, Takamatsu S, Minehira T, Sobajima T, Nakayama K, Kamada Y, Miyoshi E.
  • Journal Title: World J Gastroenterol Volume: 21 Issue: 13 Pages: 3876-87
  • DOI: 10.3748/wjg.v21.i13.3876
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Secreted frizzled-related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis. (2015)
  • Author(s): Chatani N, Kamada Y, Kizu T, Ogura S, Furuta K, Egawa M, Hamano M, Ezaki H, Kiso S, Shimono A, Ouchi N, Yoshida Y, Takehara T.
  • Journal Title: Liver Int Volume: in press Issue: 8 Pages: 2017-26
  • DOI: 10.1111/liv.12757
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] N-Acetylglucosaminyltransferase V exacerbates concanavalin A‑induced hepatitis in mice. (2015)
  • Author(s): Kamada Y, Sato M, Kida S, Akita M, Mizutani K, Fujii H, Sobajima T, Yoshida Y, Shinzaki S, Takamatsu S, Takehara T, Miyoshi E.
  • Journal Title: Mol Med Rep. Volume: 5 Issue: 5 Pages: 3573-84
  • DOI: 10.3892/mmr.2015.3168
  • Peer Reviewed / Open Access
• [Journal Article] Serum fucosylated haptoglobin in chronic liver diseases as a potential biomarker of hepatocellular carcinoma development. (2015)
  • Author(s): Kamada Y*, Asazawa H* (equally contributed), Takeda Y, Takamatsu S, Shinzaki S, Kim Y, Nezu R, Kuzushita N, Mita E, Kat M, Miyoshi E.
  • Journal Title: Clin Chem Lab Med Volume: 53 Issue: 1 Pages: 95-102
  • DOI: 10.1515/cclm-2014-0427
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Fetuin-A negatively correlates with liver and vascular fibrosis in nonalcoholic fatty liver disease subjects. (2015)
  • Author(s): Kamada Y*, Sato M* (equally contributed), Takeda Y, Kida S, Ohara Y, Fujii H, Akita M, Mizutani K, Yoshida Y, Yamada M, Hougaku H, Takehara T, Miyoshi E.
  • Journal Title: Liver Int Volume: 35 Issue: 3 Pages: 925-35
  • DOI: 10.1111/liv.12478
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 糖鎖を標的にした新しいHBV治療開発のための基礎的検討 (2018)
  • Author(s): 鎌田佳宏、竹原徹郎、三善英知
  • Organizer: 第104回日本消化器病学会総会
• [Presentation] コアフコシル化を標的とした新規NASH治療法の開発 (2018)
  • Author(s): 鎌田佳宏、竹原徹郎、三善英知
  • Organizer: 第54回日本肝臓学会総会
• [Presentation] NAFLD/NASH診断糖鎖バイオマーカーの開発 (2018)
  • Author(s): 鎌田佳宏、三善英知
  • Organizer: 第50回日本動脈硬化学会総会
• [Presentation] 糖鎖研究でNAFLDを斬る！！ (2018)
  • Author(s): 鎌田佳宏
  • Organizer: 日本消化器病学会 近畿支部第109回例会
  • Invited
• [Presentation] NAFLDバイオマーカーとしてのMac-2bpの有用性について (2018)
  • Author(s): 鎌田佳宏、西田真由、内藤有紀子、藤好杏奈、松野衣里子、矢木椋子、北康平、高松真二、三善英知
  • Organizer: 第5回肝臓と糖尿病・代謝研究会
• [Presentation] コアフコシル化を標的としたNASH糖鎖治療法の開発 (2018)
  • Author(s): 鎌田佳宏、竹原徹郎、三善英知
  • Organizer: JDDW 2018
• [Presentation] マウスMac-2bpの測定系開発と発現制御に関する検討 (2017)
  • Author(s): 鎌田佳宏、竹原徹郎、三善英知
  • Organizer: 第103回日本消化器病学会総会
• [Presentation] Biomarkers for NAFLD/NASH (2017)
  • Author(s): Yoshihiro Kamada, Tetsuo Takehara, Eiji Miyoshi
  • Organizer: The 103rd General Meeting of the Japanese Society of Gastroenterology
  • Int'l Joint Research / Invited
• [Presentation] NAFLD病態進展におけるMac-2 binding proteinの意義について (2017)
  • Author(s): 鎌田佳宏、竹原徹郎、三善英知
  • Organizer: 第53回日本肝臓学会総会
• [Presentation] NAFLD診断におけるMac-2 binding proteinの有用性について (2017)
  • Author(s): 鎌田佳宏、竹原徹郎、三善英知
  • Organizer: 日本消化器病学会近畿支部第107回例会
• [Presentation] NASH診療におけるMac-2 binding proteinとM2BPGiの比較検討 (2017)
  • Author(s): 鎌田佳宏、竹原徹郎、三善英知
  • Organizer: JDDW 2017
• [Presentation] コアフコシル化の肝細胞コレステロール代謝における役割 (2017)
  • Author(s): 鎌田佳宏、竹原徹郎、三善英知
  • Organizer: 第42回日本肝臓学会西部会
• [Presentation] Establishment of mouse Mac-2 binding protein ELISA and its application for mouse chronic liver disease models. (2017)
  • Author(s): Yoshihiro Kamada, Ayumi Iwata, Yusuke Ebisutani, Akiko Yamamoto, Yui Ueda, Hitomi Arai, Hironobu Fujii, Shinji Takamatsu, Nobuhiro Maruyama, Masahiro Maeda, Tetsuo Takehara, Eiji Miyoshi
  • Organizer: The 68 th Annual Meeting of the American Association for the Study of Liver Diseases
  • Int'l Joint Research
• [Presentation] コアフコースを標的とした糖鎖治療法開発の基礎的検討 (2016)
  • Author(s): 鎌田佳宏、竹原徹郎、三善英知
  • Organizer: 第41回日本肝臓学会東部会
  • Place of Presentation: 京王プラザホテル 東京
  • Year and Date: 2016-12-08
• [Presentation] Mac-2 binding proteinのNASHバイオマーカーとしての意義 (2016)
  • Author(s): 鎌田佳宏、竹原徹郎、三善英知
  • Organizer: JDDW 2016
  • Place of Presentation: 神戸国際会議場 神戸
  • Year and Date: 2016-11-03
• [Presentation] 膵がん、慢性膵炎に対する新規糖鎖バイオマーカーの開発 (2016)
  • Author(s): 鎌田佳宏、竹原徹郎、三善英知
  • Organizer: JDDW 2016
  • Place of Presentation: 神戸国際会議場 神戸
  • Year and Date: 2016-11-03
• [Presentation] コアフコシル化を標的とした新規NASH治療法の開発の基礎的検討 (2016)
  • Author(s): 鎌田佳宏、竹原徹郎、三善英知
  • Organizer: JDDW 2016
  • Place of Presentation: 神戸国際会議場 神戸
  • Year and Date: 2016-11-03
• [Presentation] 非アルコール性脂肪性肝炎診断に有用な画期的新規抗体の開発 (2016)
  • Author(s): 鎌田佳宏、三善英知
  • Organizer: 第63回日本臨床検査医学会学術集会
  • Place of Presentation: 神戸国際会議場 神戸
  • Year and Date: 2016-09-01
• [Presentation] 炎症性腸疾患の新しい病態解明から臨床糖鎖免疫学の創成 (2016)
  • Author(s): 鎌田佳宏、藤井宏修、新崎信一郎、高松真二、三善英知
  • Organizer: 第35回日本糖質学会年会
  • Place of Presentation: 高知市文化プラザかるぽーと 高知
  • Year and Date: 2016-09-01
• [Presentation] 血液糖鎖バイオマーカーを使ったBallooning hepatocyteの診断予測 (2016)
  • Author(s): 鎌田佳宏、竹原徹郎、三善英知
  • Organizer: 第52回日本肝臓学会総会
  • Place of Presentation: ホテルニューオータニ幕張 千葉
  • Year and Date: 2016-05-19
• [Presentation] レクチンを用いた新規NASH治療法開発への基礎的検討 (2016)
  • Author(s): 鎌田佳宏、竹原徹郎、三善英知
  • Organizer: 第102回日本消化器病学会総会
  • Place of Presentation: 京王プラザホテル 東京
  • Year and Date: 2016-04-21
• [Presentation] コアフコシル化のNASH病態進展に及ぼす影響について (2015)
  • Author(s): 鎌田佳宏、竹原徹郎、三善英知
  • Organizer: 第41回肝臓学会西部会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-12-03
• [Presentation] A novel noninvasive diagnostic method for nonalcoholic steatohepatitis (NASH) using two glycobiomarkers (2015)
  • Author(s): Yoshihiro Kamada, Masafumi Ono, Hideyuki Hyogo, Hideki Fujii, Yoshio Sumida, Kojiroh Mori, Saiyu Tanaka, Makoto Yamada, Maaya Akita, Kayo Mizutani, Hironobu Fujii, Akiko Yamamoto, Shinji Takamatsu, Yuichi Yoshida, Yoshito Ito, Norifumi Kawada, Kazuaki Chayama, Toshiji Saibara, Tetsuo Takehara, and Eiji Miyoshi
  • Organizer: AASLD The Liver Meeting 2015
  • Place of Presentation: San Francisco
  • Year and Date: 2015-11-13
  • Int'l Joint Research
• [Presentation] Ectopic expression of N-acetylglucosaminyltransferase V (GnT-V) accelerates hepatic triglyceride synthesis (2015)
  • Author(s): Yusuke Ebisutani, Yoshihiro Kamada, Sachiho Kida, Kayo Mizutani, Maaya Akita, Akiko Yamamoto, Hironobu Fujii, Tomoaki Sobajima, Naoko Terao, Shinji Takamatsu, Yuichi Yoshida, Tetsuo Takehara, Eiji Miyoshi
  • Organizer: AASLD The Liver Meeting 2015
  • Place of Presentation: San Francisco
  • Year and Date: 2015-11-13
  • Int'l Joint Research
• [Presentation] 糖転移酵素N-アセチルグルコサミン転移酵素V（GnT-V）の糖・脂質代謝への影響 (2015)
  • Author(s): 鎌田佳宏、戎谷友佑、木田祥穂、山本晃子、藤井宏修、高松真二、吉田雄一、竹原徹郎、三善英知
  • Organizer: JDDW 2015
  • Place of Presentation: グランドプリンスホテル新高輪
  • Year and Date: 2015-10-08
• [Presentation] N-アセチルグルコサミン転移酵素V（GnT-V）のConcanavalin A誘導性肝炎における役割 (2015)
  • Author(s): 山本晃子、鎌田佳宏、佐藤元哉、木田祥穂、藤井宏修、三善英知
  • Organizer: 第24回Cell Death学会学術集会
  • Place of Presentation: 大阪大学会館
  • Year and Date: 2015-07-11
• [Presentation] 血清糖鎖バイオマーカー組み合わせによる非侵襲的NASH診断法～validation study (2015)
  • Author(s): 鎌田佳宏、竹原徹郎、三善英知
  • Organizer: 第51回日本肝臓学会総会
  • Place of Presentation: 熊本ホテルキャッスル
  • Year and Date: 2015-05-21
• [Presentation] 糖転移酵素GnT-Vは肝臓での脂肪酸合成・VLDL産生促進を介して血中中性脂肪値を上昇させる (2015)
  • Author(s): 鎌田佳宏、戎谷友佑、水谷佳代、木田祥穂、山本晃子、秋田真彩、藤井宏修、高松真二、吉田雄一、竹原徹郎、三善英知
  • Organizer: 第2回肝臓と糖尿病・代謝研究会
  • Place of Presentation: シーモールパレス、下関
• [Book] Glycobiology: Biology and Medicine (2015)
  • Author(s): Miyoshi E and Kamada Y
  • Total Pages: 1568
  • Publisher: Springer
• [Patent(Industrial Property Rights)] 病理診断装置、画像処理方法及びプログラム (2018)
  • Inventor(s): 三善英知、鎌田佳宏、鈴木 貴、板野景子
  • Industrial Property Rights Holder: 三善英知、鎌田佳宏、鈴木 貴、板野景子
  • Industrial Property Rights Type: 特許
  • Filing Date: 2018
  • Acquisition Date: 2018
• [Patent(Industrial Property Rights)] 消化器癌の判定方法 (2018)
  • Inventor(s): 三善英知、鎌田佳宏、高松真二、黒澤竜雄
  • Industrial Property Rights Holder: 三善英知、鎌田佳宏、高松真二、黒澤竜雄
  • Industrial Property Rights Type: 特許
  • Filing Date: 2018
  • Acquisition Date: 2018
• [Patent(Industrial Property Rights)] マルチバイオマーカーによるNASH判定法 (2016)
  • Inventor(s): 三善英知、鎌田佳宏、伊藤行夫、南 俊夫
  • Industrial Property Rights Holder: 三善英知、鎌田佳宏、伊藤行夫、南 俊夫
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-221813
  • Filing Date: 2016-11-14