| Project/Area Number |
15H04865
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Collagenous pathology/Allergology
|
|---|
| Research Institution | Hyogo Medical University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
福岡 あゆみ 兵庫医科大学, 医学部, 助教 (30709754)
安田 好文 兵庫医科大学, 医学部, 講師 (50333539)
松下 一史 兵庫医科大学, 医学部, 講師 (20581549)
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2015: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
|
|---|
| Keywords | 食物アレルギー / 経皮感作 / 免疫寛容 / IL-33 / Treg細胞 / アレルギー / 免疫学 |
|---|
| Outline of Final Research Achievements |
We established "Epicutaneous sensitized food allergy mouse model" which closely resembles the characteristics of food allergy in infants, analyzed its onset mechanism and developed its control method. As a result, when ovalbumin (OVA) was applied after weakening the skin barrier, mice increased OVA-specific IgE(OVA-IgE) levels and showed anaphylactic symptoms with decreased core-body temperature immediately after oral challenge with OVA. Basophil-depleted or TSLP-receptor-deficient mice did not produce OVA-IgE and were protected from oral challenge-induced anaphylaxis. On the other hand, IL-33-deficient mice produced normal levels of OVA-IgE, however, they were protected from anaphylaxis. Administration of OVA intragastrically before epicutaneous sensitization induced OVA-specific oral tolerance. These mice showed Foxp3 mRNA up-regulation in the inguinal LNs after epicutaneous OVA application, as a result, they were protected completely from oral challenge-induced anaphylaxis.
|
