B cell and autoantibody in the pathogenesis of dermatomyositis

• Project/Area Number: 15H04885
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: University of Tsukuba
• Principal Investigator: Fujimoto Manabu 筑波大学, 医学医療系, 教授 (90272591)
• Research Collaborator: OKIYAMA Naoko 筑波大学, 医学医療系, 講師 ; MATSUSHITA Takashi 金沢大学, 医薬保健研究域, 講師
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2015: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 免疫 / 皮膚 / 膠原病 / B細胞 / 自己免疫 / 免疫学 / 皮膚免疫

Outline of Final Research Achievements

By immunizing mice with human TIF1γ protein together with complete adjuvant, it was confirmed that anti-TIF1γ antibody was produced in C56BL/6 wild type mice, and antigen-specific T cells were also induced. It was histologically confirmed that myositis is induced in wild type mice. No symptoms such as muscle weakness or dermatitis appeared. It was confirmed that myositis was induced in naive mice by transfer of T cells from mice in which myositis was induced. By contrast, myositis was not caused by transfer of purified IgG from immunized mice. However, in mice deficient in CD19, myositis was not induced, suggesting that B cells also had some role.

Research Products

• [Journal Article] Blockade of p38 Mitogen-Activated Protein Kinase Inhibits Murine Sclerodermatous Chronic Graft-versus-Host Disease. (2017)
  • Author(s): Matsushita T, Date M, Kano M, Mizumaki K, Tennichi M, Kobayashi T, Hamaguchi Y, Hasegawa M, Fujimoto M, Takehara K.
  • Journal Title: Am J Pathol Volume: 187 Issue: 4 Pages: 841-850
  • DOI: 10.1016/j.ajpath.2016.12.016
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Enzyme-linked immunosorbent assays for detection of anti-transcriptional intermediary factor-1 gamma and anti-Mi-2 autoantibodies in dermatomyositis. (2016)
  • Author(s): Fujimoto M, Murakami A, Kurei S, Okiyama N, Kawakami A, Mishima M, Sato S, Seishima M, Suda T, Mimori T, Takehara K, Kuwana M.
  • Journal Title: J Dermatol Sci Volume: 84 Pages: 272-281
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Recent advances in dermatomyositis-specific autoantibodies. (2016)
  • Author(s): Fujimoto M, Watanabe R, Ishitsuka Y, Okiyama N.
  • Journal Title: Curr Opin Rheumatol. Volume: 28 Issue: 6 Pages: 636-644
  • DOI: 10.1097/bor.0000000000000329
  • Peer Reviewed
• [Journal Article] The PI3K-Akt pathway controls IL-10-producing regulatory B cells. (2016)
  • Author(s): MatsushitaT, Huu DL, Hamaguchi Y, Hasegawa M, Naka K, Hirao A, Muramatsu M, Takehara K, Fujimoto M.
  • Journal Title: J Allergy Clin Immunol Volume: 印刷中 Issue: 4 Pages: 21496-21509
  • DOI: 10.1016/j.jaci.2015.12.1319
  • Peer Reviewed
• [Journal Article] Decreased Levels of Regulatory B cells in Patients with Systemic Sclerosis: Association with autoantibody production and disease activity. (2016)
  • Author(s): Matsushita T, Hamaguchi Y, Hasegawa M, Takehara K, Fujimoto M.
  • Journal Title: Rheumatology (Oxford). Volume: 55 Issue: 2 Pages: 263-267
  • DOI: 10.1093/rheumatology/kev331
  • NAID: 120005764209
  • Peer Reviewed / Acknowledgement Compliant